Restriction map for pF1KE0423
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbsI        BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfuI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         583  CGCG                 CG^CG
AlwNI         262  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          170  TGGCCA               TGG^CCA
BbeI          245  GGCGCC               GGCGC^C
BbrPI         508  CACGTG               CAC^GTG
BcnI          345  CCGGG                CC^[GC]GG
BpmI          484  CTGGAG               CTGGAG(16/14)
BsaAI         508  CACGTG               [CT]AC^GT[GA]
BsaI          649  GAGACC               GGTCTC(1/5)
BsgI          355  CTGCAC               GTGCAG(16/14)
BsrFI         242  ACCGGC               [GA]^CCGG[CT]
BstAPI        507  GCACGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I          6  CATCC                GGATG(2/0)
BstUI         583  CGCG                 CG^CG
CfoI          246  GCGC                 GCG^C
Cfr10I        242  ACCGGC               [GA]^CCGG[CT]
DpnII         387  GATC                 ^GATC
DraIII        508  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         633  CCTCAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          444  GCGGG                CCCGC(4/6)
FokI            6  CATCC                GGATG(9/13)
HaeII         245  GGCGCC               [GA]GCGC^[CT]
HgaI          427  GACGC                GACGC(5/10)
HhaI          246  GCGC                 GCG^C
HinP1I        246  GCGC                 G^CGC
HinfI         694  GACTC                G^A[ACGT]TC
KasI          245  GGCGCC               G^GCGCC
MaeII         509  ACGT                 A^CGT
MaeIII        341  GTAAC                ^GT[ACGT]AC
MboI          387  GATC                 ^GATC
MluNI         170  TGGCCA               TGG^CCA
MscI          170  TGGCCA               TGG^CCA
MslI          453  CACTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          583  CGCG                 CG^CG
NarI          245  GGCGCC               GG^CGCC
NciI          345  CCGGG                CC^[GC]GG
NdeII         387  GATC                 ^GATC
NruI          582  TCGCGA               TCG^CGA
PaeR7I        284  CTCGAG               C^TCGAG
PleI          694  GACTC                GAGTC(4/5)
PmaCI         508  CACGTG               CAC^GTG
PmlI          508  CACGTG               CAC^GTG
SacI          564  GAGCTC               GAGCT^C
Sau3AI        387  GATC                 ^GATC
SfoI          245  GGCGCC               GGC^GCC
SgrAI         241  CACCGGCG             C[GA]^CCGG[CT]G
SmlI          284  CTCGAG               C^T[CT][GA]AG
Tsp509I       553  AATT                 ^AATT
XhoI          284  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI          245  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
AspHI         564  GAGCTC               G[AT]GC[AT]^C
              660  GAGCAC              
AvaI           67  CCCGAG               C^[CT]CG[GA]G
              284  CTCGAG              
BanII         400  GAGCCC               G[GA]GC[CT]^C
              564  GAGCTC              
BbvI           42  GCAGC                GCAGC(8/12)
              601  GCTGC               
BsaHI         245  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
BseRI          55  CTCCTC               GAGGAG(10/8)
              586  GAGGAG              
BsiHKAI       564  GAGCTC               G[AT]GC[AT]^C
              660  GAGCAC              
BsmAI         649  GAGAC                GTCTC(1/5)
              319  GTCTC               
BsmFI         446  GGGAC                GGGAC(10/14)
              620  GGGAC               
BsoBI          67  CCCGAG               C^[CT]CG[GA]G
              284  CTCGAG              
BsrDI         129  CATTGC               GCAATG(2/0)
               11  GCAATG              
Cac8I          62  GCCTGC               GC[ACGT]^[ACGT]GC
              503  GCAGGC              
DsaI           99  CCATGG               C^C[GA][CT]GG
              204  CCATGG              
EaeI          170  TGGCCA               [CT]^GGCC[GA]
              461  TGGCCG              
EarI          181  GAAGAG               CTCTTC(1/4)
              336  GAAGAG              
EcoT14I        99  CCATGG               C^C[AT][AT]GG
              204  CCATGG              
HapII         243  CCGG                 C^CGG
              345  CCGG                
Hin1I         245  GGCGCC               G[GA]^CG[CT]C
              427  GACGCC              
HpaII         243  CCGG                 C^CGG
              345  CCGG                
HphI          215  TCACC                GGTGA(8/7)
              556  TCACC               
Ksp632I       181  GAAGAG               CTCTTC(1/4)
              336  GAAGAG              
MspI          243  CCGG                 C^CGG
              345  CCGG                
NcoI           99  CCATGG               C^CATGG
              204  CCATGG              
NspI           38  ACATGC               [GA]CATG^[CT]
              327  GCATGT              
StyI           99  CCATGG               C^C[AT][AT]GG
              204  CCATGG              
TaqI          285  TCGA                 T^CGA
              527  TCGA                
TseI           42  GCAGC                G^C[AT]GC
              601  GCTGC               
TspRI          46  CACTG                CA[GC]TG[ACGT][ACGT]^
              390  CAGTG               
TthHB8I       285  TCGA                 T^CGA
              527  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        AvaII       BanI        BbvCI       
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      ItaI        
MboII       MnlI        MvaI        MwoI        NlaIII      
NlaIV       PpuMI       Sau96I      ScrFI       StuI        
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