Restriction map for pF1KB3853
 (Linear) (Six-base) MAPSORT of: KIBB3853.seq  Check: 8053  from: 1  to: 636



KIBB3853.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     203     636 
   Size:        203     433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     614     636 
   Size:        447     167      22

  Fragments arranged by size: 

                 447     167      22
 
AgeI A'CCGG_T

Cuts at:      0     198     636 
   Size:        198     438
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     381     636 
   Size:        381     255
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     369     636 
   Size:        301      68     267

  Fragments arranged by size: 

                 301     267      68
 
ApaI G_GGCC'C

Cuts at:      0     377     636 
   Size:        377     259
 
ApaLI G'TGCA_C

Cuts at:      0     315     636 
   Size:        315     321
 
ApoI r'AATT_y

Cuts at:      0     121     436     636 
   Size:        121     315     200

  Fragments arranged by size: 

                 315     200     121
 
BamHI G'GATC_C

Cuts at:      0     160     636 
   Size:        160     476
 
BanII G_rGCy'C

Cuts at:      0     377     636 
   Size:        377     259
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339     411     620     636 
   Size:        339      72     209      16

  Fragments arranged by size: 

                 339     209      72      16
 
BbvCI CC'TCA_GC

Cuts at:      0     151     636 
   Size:        151     485
 
Bme1580I G_kGCm'C

Cuts at:      0     319     377     636 
   Size:        319      58     259

  Fragments arranged by size: 

                 319     259      58
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     183     357     636 
   Size:        183     174     279

  Fragments arranged by size: 

                 279     183     174
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     409     636 
   Size:        409     227
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     635     636 
   Size:        575      60       1

  Fragments arranged by size: 

                 575      60       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     151     636 
   Size:        151     485
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     636 
   Size:         77     559
 
BsaBI GATnn'nnATC

Cuts at:      0     331     636 
   Size:        331     305
 
BsaWI w'CCGG_w

Cuts at:      0     198     636 
   Size:        198     438
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     604     636 
   Size:        139     465      32

  Fragments arranged by size: 

                 465     139      32
 
BseYI C'CCAG_C

Cuts at:      0     476     636 
   Size:        476     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     636 
   Size:        274     362
 
BsiEI CG_ry'CG

Cuts at:      0     203     636 
   Size:        203     433
 
BsiHKAI G_wGCw'C

Cuts at:      0     319     636 
   Size:        319     317
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     523     636 
   Size:        523     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     319     377     636 
   Size:        319      58     259

  Fragments arranged by size: 

                 319     259      58
 
BsrDI GCAATG_nn'

Cuts at:      0     239     636 
   Size:        239     397
 
BsrFI r'CCGG_y

Cuts at:      0     198     636 
   Size:        198     438
 
BstYI r'GATC_y

Cuts at:      0     160     636 
   Size:        160     476
 
BtgI C'CryG_G

Cuts at:      0     248     308     636 
   Size:        248      60     328

  Fragments arranged by size: 

                 328     248      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     318     339     636 
   Size:        318      21     297

  Fragments arranged by size: 

                 318     297      21
 
BtsI GCAGTG_nn'

Cuts at:      0     262     302     636 
   Size:        262      40     334

  Fragments arranged by size: 

                 334     262      40
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     159     194     636 
   Size:        159      35     442

  Fragments arranged by size: 

                 442     159      35
 
DraI TTT'AAA

Cuts at:      0     453     636 
   Size:        453     183
 
EaeI y'GGCC_r

Cuts at:      0     128     264     279     305     636 
   Size:        128     136      15      26     331

  Fragments arranged by size: 

                 331     136     128      26      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447     575     614     635     636 
   Size:        447     128      39      21       1

  Fragments arranged by size: 

                 447     128      39      21       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     373     636 
   Size:        373     263
 
EcoRI G'AATT_C

Cuts at:      0     436     636 
   Size:        436     200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     363     636 
   Size:        363     273
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      47      79     152     184     610     636 
   Size:         47      32      73      32     426      26

  Fragments arranged by size: 

                 426      73      47      32      32      26
 
HincII GTy'rAC

Cuts at:      0     204     579     636 
   Size:        204     375      57

  Fragments arranged by size: 

                 375     204      57
 
HindIII A'AGCT_T

Cuts at:      0      95     636 
   Size:         95     541
 
MscI TGG'CCA

Cuts at:      0     130     266     281     307     636 
   Size:        130     136      15      26     329

  Fragments arranged by size: 

                 329     136     130      26      15
 
MslI CAynn'nnrTG

Cuts at:      0      67     502     636 
   Size:         67     435     134

  Fragments arranged by size: 

                 435     134      67
 
MspA1I CmG'CkG

Cuts at:      0     118     275     636 
   Size:        118     157     361

  Fragments arranged by size: 

                 361     157     118
 
NcoI C'CATG_G

Cuts at:      0     248     308     636 
   Size:        248      60     328

  Fragments arranged by size: 

                 328     248      60
 
NspI r_CATG'y

Cuts at:      0     568     636 
   Size:        568      68
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     173     369     636 
   Size:        173     196     267

  Fragments arranged by size: 

                 267     196     173
 
PspOMI G'GGCC_C

Cuts at:      0     373     636 
   Size:        373     263
 
PstI C_TGCA'G

Cuts at:      0     297     636 
   Size:        297     339
 
SalI G'TCGA_C

Cuts at:      0     202     636 
   Size:        202     434
 
SfcI C'TryA_G

Cuts at:      0     293     636 
   Size:        293     343
 
SmlI C'TyrA_G

Cuts at:      0      92     636 
   Size:         92     544
 
StyI C'CwwG_G

Cuts at:      0     248     308     636 
   Size:        248      60     328

  Fragments arranged by size: 

                 328     248      60
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     274     636 
   Size:        274     362
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AlfI    AlwNI     ApaI    ApaLI     ApoI
    BamHI    BanII     BbsI    BbvCI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BsrDI    BsrFI    BstYI     BtgI    BtgZI
     BtsI    CspCI     DraI     EaeI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     NcoI     NspI
    PflMI   PspOMI     PstI     SalI     SfcI     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BsaI    BsaAI    BsaHI    BsaXI    BsiWI
     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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