Restriction map for pF1KA1428
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AvaII       AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BlnI        
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MspA1I      MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Van91I      
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII         426  CGCG                 CG^CG
AflII        1134  CTTAAG               C^TTAAG
AocI         1289  CCTTAGG              CC^T[ACGT]AGG
AseI          430  ATTAAT               AT^TAAT
AsnI          430  ATTAAT               AT^TAAT
Asp700I       391  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         246  GAGCTC               G[AT]GC[AT]^C
AspI           54  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1592  CCCGGG               C^[CT]CG[GA]G
BamHI        1285  GGATCC               G^GATCC
BanII         246  GAGCTC               G[GA]GC[CT]^C
BbsI         1006  GAAGAC               GAAGAC(2/6)
BciVI         844  GGATAC               GTATCC(6/5)
BclI          414  TGATCA               T^GATCA
BfrI         1134  CTTAAG               C^TTAAG
BglI         1418  GCCAGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         363  AGATCT               A^GATCT
BlpI          285  GCTTAGC              GC^T[ACGT]AGC
BmyI          246  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      285  GCTTAGC              GC^T[ACGT]AGC
BpuAI        1006  GAAGAC               GAAGAC(2/6)
BsaBI        1543  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          733  GAGACC               GGTCTC(1/5)
BseRI          28  GAGGAG               GAGGAG(10/8)
BsiHKAI       246  GAGCTC               G[AT]GC[AT]^C
BsmBI          31  GAGACG               CGTCTC(1/5)
BsmFI         890  GGGAC                GGGAC(10/14)
BsoBI        1592  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      246  GAGCTC               G[AGT]GC[ACT]^C
BsrDI         254  CATTGC               GCAATG(2/0)
BstUI         426  CGCG                 CG^CG
Bsu36I       1289  CCTTAGG              CC^T[ACGT]AGG
CelII         285  GCTTAGC              GC^T[ACGT]AGC
Eco81I       1289  CCTTAGG              CC^T[ACGT]AGG
FbaI          414  TGATCA               T^GATCA
HaeII        1742  AGCGCC               [GA]GCGC^[CT]
HgaI           33  GACGC                GACGC(5/10)
MaeII        1693  ACGT                 A^CGT
MamI         1543  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI         1549  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          426  CGCG                 CG^CG
NcoI          965  CCATGG               C^CATGG
NlaIV        1285  GGATCC               GG[ACGT]^[ACGT]CC
PflFI          54  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         430  ATTAAT               AT^TAAT
SacI          246  GAGCTC               GAGCT^C
SmaI         1592  CCCGGG               CCC^GGG
SmlI         1134  CTTAAG               C^T[CT][GA]AG
SphI         1881  GCATGC               GCATG^C
SspI          682  AATATT               AAT^ATT
StuI          957  AGGCCT               AGG^CCT
Tth111I        54  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1326  TCTAGA               T^CTAGA
XmaCI        1592  CCCGGG               C^CCGGG
XmaI         1592  CCCGGG               C^CCGGG
XmnI          391  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1302  GTCTAC               GT^[AC][GT]AC
             2021  GTAGAC              
BpmI           37  CTGGAG               CTGGAG(16/14)
             1454  CTGGAG              
BsgI         1160  CTGCAC               GTGCAG(16/14)
              164  GTGCAG              
BsmAI          31  GAGAC                GTCTC(1/5)
              733  GAGAC               
Bsp1407I      500  TGTACA               T^GTACA
             1102  TGTACA              
BspHI         417  TCATGA               T^CATGA
             1086  TCATGA              
BsrGI         500  TGTACA               T^GTACA
             1102  TGTACA              
BssSI        1163  CACGAG               CACGAG(-5/-1)
             1222  CACGAG              
CfoI         1575  GCGC                 GCG^C
             1743  GCGC                
Cfr13I        936  GGCCC                G^G[ACGT]CC
             1595  GGGCC               
DsaI          939  CCGTGG               C^C[GA][CT]GG
              965  CCATGG              
EcoT14I       965  CCATGG               C^C[AT][AT]GG
             1518  CCTTGG              
EcoT22I       130  ATGCAT               ATGCA^T
              535  ATGCAT              
FauI           48  CCCGC                CCCGC(4/6)
             1775  GCGGG               
HapII           4  CCGG                 C^CGG
             1593  CCGG                
HhaI         1575  GCGC                 GCG^C
             1743  GCGC                
HinP1I       1575  GCGC                 G^CGC
             1743  GCGC                
HpaII           4  CCGG                 C^CGG
             1593  CCGG                
MspI            4  CCGG                 C^CGG
             1593  CCGG                
NsiI          130  ATGCAT               ATGCA^T
              535  ATGCAT              
NspI          608  ACATGC               [GA]CATG^[CT]
             1881  GCATGC              
RcaI          417  TCATGA               T^CATGA
             1086  TCATGA              
Sau96I        936  GGCCC                G^G[ACGT]CC
             1595  GGGCC               
SspBI         500  TGTACA               T^GTACA
             1102  TGTACA              
StyI          965  CCATGG               C^C[AT][AT]GG
             1518  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BalI        BbvI        BcnI        BfaI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       DdeI        
DpnII       DraI        EaeI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MvaI        MwoI        NciI        NdeII       
NlaIII      PleI        RsaI        Sau3AI      ScrFI       
SfaNI       TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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