Restriction map for pF1KE0482
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        Hin1I       HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tth111I     TthHB8I     
Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI          856  GTATAC               GT^[AC][GT]AC
ApaI          698  GGGCCC               GGGCC^C
AvaI          776  CCCGGG               C^[CT]CG[GA]G
AvaII         324  GGTCC                G^G[AT]CC
BamHI         800  GGATCC               G^GATCC
BglII         959  AGATCT               A^GATCT
BpmI          931  CTGGAG               CTGGAG(16/14)
BsoBI         776  CCCGGG               C^[CT]CG[GA]G
Bst1107I      856  GTATAC               GTA^TAC
BstXI         497  CCAGCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       856  GTATAC               GTA^TAC
CfoI          371  GCGC                 GCG^C
DraII         697  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      697  AGGGCCC              [GA]G^G[ACGT]CC[CT]
HaeII         370  GGCGCT               [GA]GCGC^[CT]
HhaI          371  GCGC                 GCG^C
HinP1I        371  GCGC                 G^CGC
HincII        847  GTCAAC               GT[CT]^[GA]AC
HindII        847  GTCAAC               GT[CT]^[GA]AC
HphI          491  TCACC                GGTGA(8/7)
MaeII         350  ACGT                 A^CGT
MslI          871  CACTTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          182  CATATG               CA^TATG
PstI          446  CTGCAG               CTGCA^G
SacI          949  GAGCTC               GAGCT^C
SfcI          446  CTGCAG               C^T[GA][CT]AG
SmaI          776  CCCGGG               CCC^GGG
XmaCI         776  CCCGGG               C^CCGGG
XmaI          776  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          121  GCGG                 CCGC(-3/-1)
              915  GCGG                
Asp700I       105  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              289  GAAAAGCTTC          
AspHI         508  GAGCAC               G[AT]GC[AT]^C
              949  GAGCTC              
BanI          411  GGCACC               G^G[CT][GA]CC
              769  GGCACC              
BbvCI         901  GCTGAGG              CCTCAGC(-5/-2)
              909  GCTGAGG             
BsgI          336  CTGCAC               GTGCAG(16/14)
              208  GTGCAG              
BsiHKAI       508  GAGCAC               G[AT]GC[AT]^C
              949  GAGCTC              
BsmFI         427  GGGAC                GGGAC(10/14)
              390  GTCCC               
BstF5I        138  GGATG                GGATG(2/0)
              840  GGATG               
FokI          138  GGATG                GGATG(9/13)
              840  GGATG               
HgaI          472  GCGTC                GACGC(5/10)
              969  GCGTC               
HindIII       172  AAGCTT               A^AGCTT
              292  AAGCTT              
MseI          584  TTAA                 T^TAA
              629  TTAA                
MspA1I        449  CAGCTG               C[AC]G^C[GT]G
              731  CAGCTG              
PvuII         449  CAGCTG               CAG^CTG
              731  CAGCTG              
Tru9I         584  TTAA                 T^TAA
              629  TTAA                
Tsp509I       128  AATT                 ^AATT
              634  AATT                
XcmI          341  CCATCTCCAACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              494  CCACCAGCATCGTGG     
XmnI          105  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              289  GAAAAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AluI        AlwI        AlwNI       BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstAPI      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      HaeIII      
HapII       HinfI       HpaII       ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        TseI        Tsp45I      
TspRI       XhoII       
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