Restriction map for
pF1KE0482
(Circular) (Six-base) MAPSORT of: pF1KE0482.seq Check: 4672 from: 1 to: 4106
pF1KE0482 4106 bp
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 965 1144 965
Size: 179 3927
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 295 665 911 1931 2363 3110 3378 155
Size: 140 370 246 1020 432 747 268 883
Fragments arranged by size:
1020 883 747 432 370 268 246 140
AflIII A'CryG_T
Cuts at: 1458 2562 3539 1458
Size: 1104 977 2025
Fragments arranged by size:
2025 1104 977
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 443 583 643 1038 1080 1411 2978 443
Size: 140 60 395 42 331 1567 1571
Fragments arranged by size:
1571 1567 395 331 140 60 42
ApaI G_GGCC'C
Cuts at: 810 810
Size: 4106
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 884 1126 884
Size: 242 3864
BamHI G'GATC_C
Cuts at: 908 1131 908
Size: 223 3883
BanI G'GyrC_C
Cuts at: 519 877 1122 1536 1784 1819 519
Size: 358 245 414 248 35 2806
Fragments arranged by size:
2806 414 358 248 245 35
BanII G_rGCy'C
Cuts at: 810 906 1061 1120 2150 810
Size: 96 155 59 1030 2766
Fragments arranged by size:
2766 1030 155 96 59
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbvCI CC'TCA_GC
Cuts at: 1010 1018 1010
Size: 8 4098
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 1067 1621 3658 1067
Size: 554 2037 1515
Fragments arranged by size:
2037 1515 554
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 411 810 1731 1824 2880 411
Size: 399 921 93 1056 1637
Fragments arranged by size:
1637 1056 921 399 93
BmrI ACTGGGnnnn_n'
Cuts at: 166 602 1318 1399 1734 3878 166
Size: 436 716 81 335 2144 394
Fragments arranged by size:
2144 716 436 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1060
Size: 4106
Bpu10I CC'TnA_GC
Cuts at: 1010 1018 1031 1010
Size: 8 13 4085
Fragments arranged by size:
4085 13 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 914 1027 1057 914
Size: 113 30 3963
Fragments arranged by size:
3963 113 30
BseYI C'CCAG_C
Cuts at: 559 570 1531 2289 2866 3362 559
Size: 11 961 758 577 496 1303
Fragments arranged by size:
1303 961 758 577 496 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 429 337
Size: 92 4014
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 620 1061 1120 1898 2088 2880 620
Size: 441 59 778 190 792 1846
Fragments arranged by size:
1846 792 778 441 190 59
Bsp1286I G_dGCh'C
Cuts at: 411 620 810 906 1061 1120 1731 1824 1898
Size: 209 190 96 155 59 611 93 74
Cuts at: 1898 2088 2150 2880 411
Size: 190 62 730 1637
Fragments arranged by size:
1637 730 611 209 190 190 155 96
93 74 62 59
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 189 583 1000 1483 189
Size: 394 417 483 2812
Fragments arranged by size:
2812 483 417 394
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 612 612
Size: 4106
BstYI r'GATC_y
Cuts at: 1 380 908 1067 1131 1598 1621 1956 2202
Size: 379 528 159 64 467 23 335 246
Cuts at: 2202 3214 3300 3312 3658 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 528 467 449 379 346 335 246
159 86 64 23 12
BstZ17I GTA'TAC
Cuts at: 966 966
Size: 4106
BtgI C'CryG_G
Cuts at: 107 107
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 896 1349 896
Size: 453 3653
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1691 1865 2256 2283 3628 1691
Size: 174 391 27 1345 2169
Fragments arranged by size:
2169 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 131 208 422 2129 2339 131
Size: 77 214 1707 210 1898
Fragments arranged by size:
1898 1707 214 210 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1059 1118 1059
Size: 59 4047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 295 665 911 1060 1931 2363 3110 3378
Size: 140 370 246 149 871 432 747 268
Cuts at: 3378 155
Size: 883
Fragments arranged by size:
883 871 747 432 370 268 246 149
140
EcoO109I rG'GnC_Cy
Cuts at: 806 1245 806
Size: 439 3667
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 144 2125 144
Size: 1981 2125
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 482 1540 1788 2810 3789 482
Size: 1058 248 1022 979 799
Fragments arranged by size:
1058 1022 979 799 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 893 925 1948 1978 1980 2010 893
Size: 32 1023 30 2 30 2989
Fragments arranged by size:
2989 1023 32 30 30 2
HincII GTy'rAC
Cuts at: 957 1145 957
Size: 188 3918
HindIII A'AGCT_T
Cuts at: 280 400 280
Size: 120 3986
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 479 2777 2961 479
Size: 2298 184 1624
Fragments arranged by size:
2298 1624 184
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 983 2095 2222 983
Size: 1112 127 2867
Fragments arranged by size:
2867 1112 127
MspA1I CmG'CkG
Cuts at: 559 841 1217 1531 1891 2904 3149 559
Size: 282 376 314 360 1013 245 1516
Fragments arranged by size:
1516 1013 376 360 314 282 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NdeI CA'TA_TG
Cuts at: 291 291
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 1159 1659 2566 1159
Size: 500 907 2699
Fragments arranged by size:
2699 907 500
PasI CC'CwG_GG
Cuts at: 242 242
Size: 4106
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PfoI T'CCnGG_A
Cuts at: 155 383 155
Size: 228 3878
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PspOMI G'GGCC_C
Cuts at: 806 806
Size: 4106
PstI C_TGCA'G
Cuts at: 558 1153 1838 558
Size: 595 685 2826
Fragments arranged by size:
2826 685 595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 559 841 1531 1891 559
Size: 282 690 360 2774
Fragments arranged by size:
2774 690 360 282
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1061 1120 1061
Size: 59 4047
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 554 1149 1834 2827 3018 33
Size: 521 595 685 993 191 1121
Fragments arranged by size:
1121 993 685 595 521 191
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 886 1128 886
Size: 242 3864
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 107 1240 1594 107
Size: 1133 354 2619
Fragments arranged by size:
2619 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 692 732 1689 1822 2281 2490 692
Size: 40 957 133 459 209 2308
Fragments arranged by size:
2308 957 459 209 133 40
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 932 3623 3655 900
Size: 32 2691 32 1351
Fragments arranged by size:
2691 1351 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 609 1601 456
Size: 153 992 2961
Fragments arranged by size:
2961 992 153
XmaI C'CCGG_G
Cuts at: 884 1126 884
Size: 242 3864
XmnI GAAnn'nnTTC
Cuts at: 217 401 217
Size: 184 3922
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI