################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BbsI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FbaI FseI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TspRI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 430 CGCG CG^CG AcyI 632 GGCGCC G[GA]^CG[CT]C AflIII 828 ACACGT A^C[GA][CT]GT ApaI 914 GGGCCC GGGCC^C AviII 839 TGCGCA TGC^GCA BamHI 579 GGATCC G^GATCC BbeI 632 GGCGCC GGCGC^C BbrPI 829 CACGTG CAC^GTG BciVI 274 GTATCC GTATCC(6/5) BfaI 1009 CTAG C^TAG BglII 737 AGATCT A^GATCT BsaAI 829 CACGTG [CT]AC^GT[GA] BsaHI 632 GGCGCC G[GA]^CG[CT]C BseRI 1012 GAGGAG GAGGAG(10/8) BsgI 541 CTGCAC GTGCAG(16/14) BsmI 535 GCATTC GAATGC(1/-1) Bsp1407I 482 TGTACA T^GTACA BspHI 929 TCATGA T^CATGA BsrGI 482 TGTACA T^GTACA BsrI 377 ACTGG ACTGG(1/-1) BstUI 430 CGCG CG^CG EaeI 311 TGGCCG [CT]^GGCC[GA] EcoNI 876 CCTATATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 316 GAATTC G^AATTC EcoT14I 508 CCTTGG C^C[AT][AT]GG EcoT22I 533 ATGCAT ATGCA^T FspI 839 TGCGCA TGC^GCA Hin1I 632 GGCGCC G[GA]^CG[CT]C KasI 632 GGCGCC G^GCGCC MaeI 1009 CTAG C^TAG MaeII 830 ACGT A^CGT MseI 605 TTAA T^TAA MspA1I 235 CAGCTG C[AC]G^C[GT]G MvnI 430 CGCG CG^CG NarI 632 GGCGCC GG^CGCC NsiI 533 ATGCAT ATGCA^T PflMI 696 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 414 GAGTC GAGTC(4/5) PmaCI 829 CACGTG CAC^GTG PmlI 829 CACGTG CAC^GTG PpuMI 683 GGGTCCT [GA]G^G[AT]CC[CT] PstI 405 CTGCAG CTGCA^G PvuII 235 CAGCTG CAG^CTG RcaI 929 TCATGA T^CATGA ScaI 950 AGTACT AGT^ACT SexAI 265 ACCTGGT A^CC[AT]GGT SfoI 632 GGCGCC GGC^GCC SspBI 482 TGTACA T^GTACA StyI 508 CCTTGG C^C[AT][AT]GG Tru9I 605 TTAA T^TAA Van91I 696 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 580 GATCC GGATC(4/5) 579 GGATC AspHI 328 GAGCAC G[AT]GC[AT]^C 1015 GAGCAC BanI 632 GGCGCC G^G[CT][GA]CC 676 GGCACC BpmI 1054 CTCCAG CTGGAG(16/14) 765 CTGGAG BsiHKAI 328 GAGCAC G[AT]GC[AT]^C 1015 GAGCAC BsrBI 132 CCGCTC CCGCTC(-3/-3) 28 GAGCGG BstAPI 498 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 894 GCAAATGCTGC BstYI 579 GGATCC [GA]^GATC[CT] 737 AGATCT DraII 683 GGGTCCT [GA]G^G[ACGT]CC[CT] 913 GGGGCCC DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 613 GACCCTTTGGTC EcoO109I 683 GGGTCCT [GA]G^G[ACGT]CC[CT] 913 GGGGCCC HphI 263 TCACC GGTGA(8/7) 341 TCACC MaeIII 340 GTCAC ^GT[ACGT]AC 748 GTCAC MflI 579 GGATCC [GA]^GATC[CT] 737 AGATCT SfcI 405 CTGCAG C^T[GA][CT]AG 741 CTACAG Tsp45I 340 GTCAC ^GT[GC]AC 748 GTCAC XcmI 993 CCAGCCCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1023 CCAAGCCCTGAGTGG XhoII 579 GGATCC [GA]^GATC[CT] 737 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp509I TthHB8I