Restriction map for pF1KB5971
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BbrPI       
BciVI       BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspEI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoT14I     EcoT22I     
FseI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         1062  GTCGAC               GT^[AC][GT]AC
AccII           7  CGCG                 CG^CG
AcyI          796  GGCGCC               G[GA]^CG[CT]C
Alw44I        972  GTGCAC               G^TGCAC
AocI          889  CCTGAGG              CC^T[ACGT]AGG
ApaLI         972  GTGCAC               G^TGCAC
AspHI         972  GTGCAC               G[AT]GC[AT]^C
AviII        1391  TGCGCA               TGC^GCA
BalI         1295  TGGCCA               TGG^CCA
BbeI          796  GGCGCC               GGCGC^C
BbvCI        1135  CCTCAGC              CCTCAGC(-5/-2)
BcgI          111  CGAGCTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          575  TGATCA               T^GATCA
BsaHI         796  GGCGCC               G[GA]^CG[CT]C
BsaI           39  GAGACC               GGTCTC(1/5)
BsgI          923  GTGCAG               GTGCAG(16/14)
BsiHKAI       972  GTGCAC               G[AT]GC[AT]^C
BsmI          640  GAATGC               GAATGC(1/-1)
BspDI         730  ATCGAT               AT^CGAT
BspHI        1262  TCATGA               T^CATGA
BspLU11I     1478  ACATGT               A^CATGT
BspMI         775  ACCTGC               ACCTGC(4/8)
BsrBI         496  GAGCGG               CCGCTC(-3/-3)
BstEII        586  GGTGACC              G^GT[ACGT]ACC
BstPI         586  GGTGACC              G^GT[ACGT]ACC
BstUI           7  CGCG                 CG^CG
Bsu36I        889  CCTGAGG              CC^T[ACGT]AGG
ClaI          730  ATCGAT               AT^CGAT
Eco81I        889  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       586  GGTGACC              G^GT[ACGT]ACC
EcoRV         124  GATATC               GAT^ATC
FbaI          575  TGATCA               T^GATCA
FspI         1391  TGCGCA               TGC^GCA
HgaI         1065  GACGC                GACGC(5/10)
Hin1I         796  GGCGCC               G[GA]^CG[CT]C
KasI          796  GGCGCC               G^GCGCC
MaeII         461  ACGT                 A^CGT
MluNI        1295  TGGCCA               TGG^CCA
MscI         1295  TGGCCA               TGG^CCA
MseI          509  TTAA                 T^TAA
MspA1I        741  CCGCTG               C[AC]G^C[GT]G
MvnI            7  CGCG                 CG^CG
NarI          796  GGCGCC               GG^CGCC
PleI         1275  GACTC                GAGTC(4/5)
PshAI         849  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1069  CTGCAG               CTGCA^G
RcaI         1262  TCATGA               T^CATGA
SalI         1062  GTCGAC               G^TCGAC
SapI         1179  GAAGAGC              GCTCTTC(1/4)
SfcI         1069  CTGCAG               C^T[GA][CT]AG
SfiI          292  GGCCTGAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          796  GGCGCC               GGC^GCC
TfiI           33  GATTC                G^A[AT]TC
Tru9I         509  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        461  ACGTGT               A^C[GA][CT]GT
             1478  ACATGT              
AlwI          417  GATCC                GGATC(4/5)
             1014  GATCC               
AvaI         1088  CCCGGG               C^[CT]CG[GA]G
             1493  CCCGGG              
BbsI          441  GAAGAC               GAAGAC(2/6)
             1272  GAAGAC              
BpuAI         441  GAAGAC               GAAGAC(2/6)
             1272  GAAGAC              
BseRI         400  CTCCTC               GAGGAG(10/8)
             1515  CTCCTC              
BsmAI          39  GAGAC                GTCTC(1/5)
              134  GTCTC               
BsmFI         842  GGGAC                GGGAC(10/14)
             1208  GTCCC               
BsoBI        1088  CCCGGG               C^[CT]CG[GA]G
             1493  CCCGGG              
BstYI         416  AGATCC               [GA]^GATC[CT]
             1013  AGATCC              
DsaI           86  CCGTGG               C^C[GA][CT]GG
               92  CCGTGG              
EaeI           89  TGGCCG               [CT]^GGCC[GA]
             1295  TGGCCA              
HaeII         796  GGCGCC               [GA]GCGC^[CT]
             1255  AGCGCC              
HincII        631  GTCAAC               GT[CT]^[GA]AC
             1062  GTCGAC              
HindII        631  GTCAAC               GT[CT]^[GA]AC
             1062  GTCGAC              
HinfI          33  GATTC                G^A[ACGT]TC
             1275  GACTC               
MflI          416  AGATCC               [GA]^GATC[CT]
             1013  AGATCC              
MslI          619  CACTGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              965  CACCTCTGTG          
NspI         1099  ACATGC               [GA]CATG^[CT]
             1478  ACATGT              
SmaI         1088  CCCGGG               CCC^GGG
             1493  CCCGGG              
SmlI          930  CTTGAG               C^T[CT][GA]AG
             1009  CTCAAG              
XhoII         416  AGATCC               [GA]^GATC[CT]
             1013  AGATCC              
XmaCI        1088  CCCGGG               C^CCGGG
             1493  CCCGGG              
XmaI         1088  CCCGGG               C^CCGGG
             1493  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       ApaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StuI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        
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