Restriction map for
pF1KB6453
(Circular) (Six-base) MAPSORT of: pF1KB6453.seq Check: 4717 from: 1 to: 4067
pF1KB6453 4067 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 715 1892 2324 3071 3339 415
Size: 300 1177 432 747 268 1143
Fragments arranged by size:
1177 1143 747 432 300 268
AfeI AGC'GCT
Cuts at: 667 667
Size: 4067
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 438 1372 2939 438
Size: 934 1567 1566
Fragments arranged by size:
1567 1566 934
ApaI G_GGCC'C
Cuts at: 232 232
Size: 4067
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 589 1071 3420 3676 589
Size: 482 2349 256 980
Fragments arranged by size:
2349 980 482 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 640 673 640
Size: 33 4034
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 869 947 1083 1497 1745 1780 869
Size: 78 136 414 248 35 3156
Fragments arranged by size:
3156 414 248 136 78 35
BanII G_rGCy'C
Cuts at: 158 232 1081 2111 158
Size: 74 849 1030 2114
Fragments arranged by size:
2114 1030 849 74
BbeI G_GCGC'C
Cuts at: 951 1501 1749 951
Size: 550 248 3269
Fragments arranged by size:
3269 550 248
BbsI GAAGACnn'nnnn_
Cuts at: 149 149
Size: 4067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 561 1029 1587 561
Size: 468 558 3041
Fragments arranged by size:
3041 558 468
BglII A'GATC_T
Cuts at: 296 1582 3619 296
Size: 1286 2037 744
Fragments arranged by size:
2037 1286 744
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 232 523 1692 1785 2841 232
Size: 291 1169 93 1056 1458
Fragments arranged by size:
1458 1169 1056 291 93
BmrI ACTGGGnnnn_n'
Cuts at: 266 1279 1360 1695 3839 266
Size: 1013 81 335 2144 494
Fragments arranged by size:
2144 1013 494 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 673
Size: 4067
Bpu10I CC'TnA_GC
Cuts at: 157 157
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1243 2122 2614 2912 277
Size: 966 879 492 298 1432
Fragments arranged by size:
1432 966 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 388 728 3771 388
Size: 340 3043 684
Fragments arranged by size:
3043 684 340
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 948 1498 1746 2448 3944 948
Size: 550 248 702 1496 1071
Fragments arranged by size:
1496 1071 702 550 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 565 568 565
Size: 3 4064
BseYI C'CCAG_C
Cuts at: 231 668 1492 2250 2827 3323 231
Size: 437 824 758 577 496 975
Fragments arranged by size:
975 824 758 577 496 437
BsiEI CG_ry'CG
Cuts at: 104 194 1609 1655 2863 104
Size: 90 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 90 46
BsiHKAI G_wGCw'C
Cuts at: 158 1081 1859 2049 2841 158
Size: 923 778 190 792 1384
Fragments arranged by size:
1384 923 792 778 190
BsmI GAATG_Cn'
Cuts at: 798 927 798
Size: 129 3938
Bsp1286I G_dGCh'C
Cuts at: 158 232 523 1081 1692 1785 1859 2049 2111
Size: 74 291 558 611 93 74 190 62
Cuts at: 2111 2841 158
Size: 730 1384
Fragments arranged by size:
1384 730 611 558 291 190 93 74
74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 291 993 1117 1331 1633 2014 291
Size: 702 124 214 302 381 2344
Fragments arranged by size:
2344 702 381 302 214 124
BsrBI CCG'CTC
Cuts at: 50 133 2359 2413 3897 4062 50
Size: 83 2226 54 1484 165 55
Fragments arranged by size:
2226 1484 165 83 55 54
BsrDI GCAATG_nn'
Cuts at: 1979 1979
Size: 4067
BsrFI r'CCGG_y
Cuts at: 659 2065 2246 2455 659
Size: 1406 181 209 2271
Fragments arranged by size:
2271 1406 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 846 1444 846
Size: 598 3469
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstYI r'GATC_y
Cuts at: 1 296 1004 1092 1559 1582 1917 2163 3175
Size: 295 708 88 467 23 335 246 1012
Cuts at: 3175 3261 3273 3619 1
Size: 86 12 346 449
Fragments arranged by size:
1012 708 467 449 346 335 295 246
88 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 1032 1310 1032
Size: 278 3789
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DraIII CAC_nnn'GTG
Cuts at: 274 274
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 191 1652 1826 2217 2244 3589 191
Size: 1461 174 391 27 1345 669
Fragments arranged by size:
1461 1345 669 391 174 27
EagI C'GGCC_G
Cuts at: 191 1652 191
Size: 1461 2606
EarI CTCTTCn'nnn_
Cuts at: 175 2090 2300 175
Size: 1915 210 1942
Fragments arranged by size:
1942 1915 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 2275 2585 2731 3877 154
Size: 2121 310 146 1146 344
Fragments arranged by size:
2121 1146 344 310 146
EcoICRI GAG'CTC
Cuts at: 156 1079 156
Size: 923 3144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 673 715 1892 2324 3071 3339 415
Size: 258 42 1177 432 747 268 1143
Fragments arranged by size:
1177 1143 747 432 268 258 42
EcoNI CCTnn'n_nnAGG
Cuts at: 241 629 241
Size: 388 3679
EcoO109I rG'GnC_Cy
Cuts at: 228 246 699 1017 1206 228
Size: 18 453 318 189 3089
Fragments arranged by size:
3089 453 318 189 18
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 669 819 951 1501 1749 2771 3750 669
Size: 150 132 550 248 1022 979 986
Fragments arranged by size:
1022 986 979 550 248 150 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 909 941 1909 1939 1941 1971 909
Size: 32 968 30 2 30 3005
Fragments arranged by size:
3005 968 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 947 1497 1745 947
Size: 550 248 3269
Fragments arranged by size:
3269 550 248
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 2056 2183 2056
Size: 127 3940
MspA1I CmG'CkG
Cuts at: 424 1178 1492 1852 2865 3110 424
Size: 754 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 754 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 948 1498 1746 948
Size: 550 248 3269
Fragments arranged by size:
3269 550 248
NdeI CA'TA_TG
Cuts at: 618 618
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PpuMI rG'GwC_Cy
Cuts at: 246 246
Size: 4067
PspOMI G'GGCC_C
Cuts at: 228 228
Size: 4067
PstI C_TGCA'G
Cuts at: 842 1114 1799 842
Size: 272 685 3110
Fragments arranged by size:
3110 685 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 424 1492 1852 424
Size: 1068 360 2639
Fragments arranged by size:
2639 1068 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 158 1081 158
Size: 923 3144
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 838 1110 1795 2788 2979 33
Size: 805 272 685 993 191 1121
Fragments arranged by size:
1121 993 805 685 272 191
SfoI GGC'GCC
Cuts at: 949 1499 1747 949
Size: 550 248 3269
Fragments arranged by size:
3269 550 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 292 1222 2137 2629 2891 292
Size: 930 915 492 262 1468
Fragments arranged by size:
1468 930 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 776 819 1013 1201 1555 776
Size: 43 194 188 354 3288
Fragments arranged by size:
3288 354 194 188 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 285 1650 1783 2242 2451 285
Size: 1365 133 459 209 1901
Fragments arranged by size:
1901 1365 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 680 680
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI