Restriction map for pF1KB6453
 (Linear) (Six-base) MAPSORT of: KIBB6453.seq  Check: 6676  from: 1  to: 954



KIBB6453.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     607     954 
   Size:        307     300     347

  Fragments arranged by size: 

                 347     307     300
 
AfeI AGC'GCT

Cuts at:      0     559     954 
   Size:        559     395
 
AlwNI CAG_nnn'CTG

Cuts at:      0     330     954 
   Size:        330     624
 
ApaI G_GGCC'C

Cuts at:      0     124     954 
   Size:        124     830
 
ApoI r'AATT_y

Cuts at:      0     481     954 
   Size:        481     473
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     532     565     954 
   Size:        532      33     389

  Fragments arranged by size: 

                 532     389      33
 
BanI G'GyrC_C

Cuts at:      0     761     839     954 
   Size:        761      78     115

  Fragments arranged by size: 

                 761     115      78
 
BanII G_rGCy'C

Cuts at:      0      50     124     954 
   Size:         50      74     830

  Fragments arranged by size: 

                 830      74      50
 
BbeI G_GCGC'C

Cuts at:      0     843     954 
   Size:        843     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      41     954 
   Size:         41     913
 
BclI T'GATC_A

Cuts at:      0     453     921     954 
   Size:        453     468      33

  Fragments arranged by size: 

                 468     453      33
 
BglII A'GATC_T

Cuts at:      0     188     954 
   Size:        188     766
 
Bme1580I G_kGCm'C

Cuts at:      0     124     415     954 
   Size:        124     291     539

  Fragments arranged by size: 

                 539     291     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     158     954 
   Size:        158     796
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     954 
   Size:        565     389
 
Bpu10I CC'TnA_GC

Cuts at:      0      49     954 
   Size:         49     905
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     954 
   Size:        169     785
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     280     620     954 
   Size:        280     340     334

  Fragments arranged by size: 

                 340     334     280
 
BsaHI Gr'CG_yC

Cuts at:      0     840     954 
   Size:        840     114
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     457     460     954 
   Size:        457       3     494

  Fragments arranged by size: 

                 494     457       3
 
BseYI C'CCAG_C

Cuts at:      0     123     560     954 
   Size:        123     437     394

  Fragments arranged by size: 

                 437     394     123
 
BsiEI CG_ry'CG

Cuts at:      0      86     954 
   Size:         86     868
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     954 
   Size:         50     904
 
BsmI GAATG_Cn'

Cuts at:      0     690     819     954 
   Size:        690     129     135

  Fragments arranged by size: 

                 690     135     129
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     124     415     954 
   Size:         50      74     291     539

  Fragments arranged by size: 

                 539     291      74      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     183     885     954 
   Size:        183     702      69

  Fragments arranged by size: 

                 702     183      69
 
BsrBI CCG'CTC

Cuts at:      0      25     954 
   Size:         25     929
 
BsrFI r'CCGG_y

Cuts at:      0     551     954 
   Size:        551     403
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     738     954 
   Size:        738     216
 
BstYI r'GATC_y

Cuts at:      0     188     896     954 
   Size:        188     708      58

  Fragments arranged by size: 

                 708     188      58
 
BtsI GCAGTG_nn'

Cuts at:      0     924     954 
   Size:        924      30
 
DraIII CAC_nnn'GTG

Cuts at:      0     166     954 
   Size:        166     788
 
EaeI y'GGCC_r

Cuts at:      0      83     954 
   Size:         83     871
 
EagI C'GGCC_G

Cuts at:      0      83     954 
   Size:         83     871
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     954 
   Size:         67     887
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46     954 
   Size:         46     908
 
EcoICRI GAG'CTC

Cuts at:      0      48     954 
   Size:         48     906
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     565     607     954 
   Size:        307     258      42     347

  Fragments arranged by size: 

                 347     307     258      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133     521     954 
   Size:        133     388     433

  Fragments arranged by size: 

                 433     388     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     138     591     909     954 
   Size:        120      18     453     318      45

  Fragments arranged by size: 

                 453     318     120      45      18
 
HaeII r_GCGC'y

Cuts at:      0     561     711     843     954 
   Size:        561     150     132     111

  Fragments arranged by size: 

                 561     150     132     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     801     833     954 
   Size:        801      32     121

  Fragments arranged by size: 

                 801     121      32
 
KasI G'GCGC_C

Cuts at:      0     839     954 
   Size:        839     115
 
MspA1I CmG'CkG

Cuts at:      0     316     954 
   Size:        316     638
 
NarI GG'CG_CC

Cuts at:      0     840     954 
   Size:        840     114
 
NdeI CA'TA_TG

Cuts at:      0     510     954 
   Size:        510     444
 
PpuMI rG'GwC_Cy

Cuts at:      0     138     954 
   Size:        138     816
 
PspOMI G'GGCC_C

Cuts at:      0     120     954 
   Size:        120     834
 
PstI C_TGCA'G

Cuts at:      0     734     954 
   Size:        734     220
 
PvuII CAG'CTG

Cuts at:      0     316     954 
   Size:        316     638
 
SacI G_AGCT'C

Cuts at:      0      50     954 
   Size:         50     904
 
SfcI C'TryA_G

Cuts at:      0     730     954 
   Size:        730     224
 
SfoI GGC'GCC

Cuts at:      0     841     954 
   Size:        841     113
 
SmlI C'TyrA_G

Cuts at:      0     184     954 
   Size:        184     770
 
StyI C'CwwG_G

Cuts at:      0     668     711     905     954 
   Size:        668      43     194      49

  Fragments arranged by size: 

                 668     194      49      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     177     954 
   Size:        177     777
 
XmnI GAAnn'nnTTC

Cuts at:      0     572     954 
   Size:        572     382
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI     ApaI     ApoI     BaeI     BanI    BanII
     BbeI     BbsI     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstYI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     KasI   MspA1I     NarI     NdeI    PpuMI   PspOMI
     PstI    PvuII     SacI     SfcI     SfoI     SmlI     StyI    TaqII
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI     DrdI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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