Restriction map for pF1KE0348
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
StyI        SwaI        Tru9I       Tth111I     XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI         1020  GAATTT               [GA]^AATT[CT]
Alw44I       1099  GTGCAC               G^TGCAC
AocI          175  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       884  AGCGCT               AGC^GCT
ApaI         1455  GGGCCC               GGGCC^C
ApaLI        1099  GTGCAC               G^TGCAC
ApoI         1020  GAATTT               [GA]^AATT[CT]
Asp700I       948  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          592  CTCGAG               C^[CT]CG[GA]G
BbeI         1253  GGCGCC               GGCGC^C
BbvCI         648  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1164  CGAGGCCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmFI         188  GGGAC                GGGAC(10/14)
BsoBI         592  CTCGAG               C^[CT]CG[GA]G
BsrDI        1088  GCAATG               GCAATG(2/0)
BssHII        587  GCGCGC               G^CGCGC
BssSI        1162  CACGAG               CACGAG(-5/-1)
Bsu36I        175  CCTGAGG              CC^T[ACGT]AGG
DrdI          507  GACCTTTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      884  AGCGCT               AGC^GCT
Eco81I        175  CCTGAGG              CC^T[ACGT]AGG
EcoNI         747  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI         1253  GGCGCC               G^GCGCC
MaeIII        959  GTGAC                ^GT[ACGT]AC
MboII         303  GAAGA                GAAGA(8/7)
NarI         1253  GGCGCC               GG^CGCC
NheI           12  GCTAGC               G^CTAGC
PaeR7I        592  CTCGAG               C^TCGAG
PflMI         777  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1312  GACCACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI          750  CCTGCAGG             CCTGCA^GG
SexAI         800  ACCTGGT              A^CC[AT]GGT
SfoI         1253  GGCGCC               GGC^GCC
Sse8387I      750  CCTGCAGG             CCTGCA^GG
StuI         1166  AGGCCT               AGG^CCT
Tsp45I        959  GTGAC                ^GT[GC]AC
Tsp509I      1021  AATT                 ^AATT
Van91I        777  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          592  CTCGAG               C^TCGAG
XmnI          948  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          910  GTCTAC               GT^[AC][GT]AC
             1393  GTCTAC              
AlwI          435  GATCC                GGATC(4/5)
              879  GATCC               
AspHI        1075  GAGCAC               G[AT]GC[AT]^C
             1099  GTGCAC              
BalI          218  TGGCCA               TGG^CCA
              638  TGGCCA              
BanII         157  GAGCCC               G[GA]GC[CT]^C
             1455  GGGCCC              
BcnI          169  CCGGG                CC^[GC]GG
              831  CCCGG               
BseRI          34  CTCCTC               GAGGAG(10/8)
              631  GAGGAG              
BsgI          229  CTGCAC               GTGCAG(16/14)
              872  GTGCAG              
BsiHKAI      1075  GAGCAC               G[AT]GC[AT]^C
             1099  GTGCAC              
BstYI         434  AGATCC               [GA]^GATC[CT]
              878  AGATCC              
HapII         169  CCGG                 C^CGG
              832  CCGG                
HgaI         1363  GACGC                GACGC(5/10)
             1391  GCGTC               
HpaII         169  CCGG                 C^CGG
              832  CCGG                
MflI          434  AGATCC               [GA]^GATC[CT]
              878  AGATCC              
MluNI         218  TGGCCA               TGG^CCA
              638  TGGCCA              
MscI          218  TGGCCA               TGG^CCA
              638  TGGCCA              
MslI           54  CATGTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              918  CATCCAGGTG          
MspI          169  CCGG                 C^CGG
              832  CCGG                
NciI          169  CCGGG                CC^[GC]GG
              831  CCCGG               
PleI          190  GACTC                GAGTC(4/5)
             1300  GACTC               
SmlI          333  CTTGAG               C^T[CT][GA]AG
              592  CTCGAG              
TfiI          211  GAATC                G^A[AT]TC
              375  GAATC               
XhoII         434  AGATCC               [GA]^GATC[CT]
              878  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaII       BanI        BbvI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HhaI        
Hin1I       HinP1I      HinfI       HphI        ItaI        
MaeI        MboI        MnlI        MspA1I      MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
TspRI       TthHB8I     XcmI        
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