Restriction map for pF1KB6464
 (Linear) (Six-base) MAPSORT of: KIBB6464.seq  Check: 4776  from: 1  to: 1056



KIBB6464.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     163    1056 
   Size:        163     893
 
AatII G_ACGT'C

Cuts at:      0     283    1056 
   Size:        283     773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     477     686    1056 
   Size:        187     290     209     370

  Fragments arranged by size: 

                 370     290     209     187
 
AfeI AGC'GCT

Cuts at:      0      54    1056 
   Size:         54    1002
 
AleI CACnn'nnGTG

Cuts at:      0     202    1056 
   Size:        202     854
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     220    1056 
   Size:        220     836
 
AlwNI CAG_nnn'CTG

Cuts at:      0     307    1056 
   Size:        307     749
 
ApaI G_GGCC'C

Cuts at:      0      18     883    1056 
   Size:         18     865     173

  Fragments arranged by size: 

                 865     173      18
 
ApoI r'AATT_y

Cuts at:      0     409    1056 
   Size:        409     647
 
AvaI C'yCGr_G

Cuts at:      0      10     600    1056 
   Size:         10     590     456

  Fragments arranged by size: 

                 590     456      10
 
BanI G'GyrC_C

Cuts at:      0     459    1056 
   Size:        459     597
 
BanII G_rGCy'C

Cuts at:      0      18     590     883    1056 
   Size:         18     572     293     173

  Fragments arranged by size: 

                 572     293     173      18
 
BbeI G_GCGC'C

Cuts at:      0     463    1056 
   Size:        463     593
 
BbvCI CC'TCA_GC

Cuts at:      0     469    1056 
   Size:        469     587
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43     698    1056 
   Size:         43     655     358

  Fragments arranged by size: 

                 655     358      43
 
BlpI GC'TnA_GC

Cuts at:      0      31    1056 
   Size:         31    1025
 
Bme1580I G_kGCm'C

Cuts at:      0      18     883    1056 
   Size:         18     865     173

  Fragments arranged by size: 

                 865     173      18
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     494    1056 
   Size:        494     562
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305    1056 
   Size:        305     751
 
Bpu10I CC'TnA_GC

Cuts at:      0     469    1056 
   Size:        469     587
 
BsaHI Gr'CG_yC

Cuts at:      0     280     460    1056 
   Size:        280     180     596

  Fragments arranged by size: 

                 596     280     180
 
BsaWI w'CCGG_w

Cuts at:      0     608    1056 
   Size:        608     448
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     289    1048    1056 
   Size:         18     271     759       8

  Fragments arranged by size: 

                 759     271      18       8
 
BseYI C'CCAG_C

Cuts at:      0     207     696    1056 
   Size:        207     489     360

  Fragments arranged by size: 

                 489     360     207
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112    1017    1056 
   Size:        112     905      39

  Fragments arranged by size: 

                 905     112      39
 
BsiEI CG_ry'CG

Cuts at:      0     710    1056 
   Size:        710     346
 
BsiHKAI G_wGCw'C

Cuts at:      0     199    1056 
   Size:        199     857
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     271    1056 
   Size:        271     785
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     199     590     883    1056 
   Size:         18     181     391     293     173

  Fragments arranged by size: 

                 391     293     181     173      18
 
BspEI T'CCGG_A

Cuts at:      0     608    1056 
   Size:        608     448
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     163     725     847    1056 
   Size:        163     562     122     209

  Fragments arranged by size: 

                 562     209     163     122
 
BsrFI r'CCGG_y

Cuts at:      0     709    1056 
   Size:        709     347
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     916    1056 
   Size:        916     140
 
BstYI r'GATC_y

Cuts at:      0     116    1056 
   Size:        116     940
 
Bsu36I CC'TnA_GG

Cuts at:      0     524    1056 
   Size:        524     532
 
BtgI C'CryG_G

Cuts at:      0     350     542     758    1056 
   Size:        350     192     216     298

  Fragments arranged by size: 

                 350     298     216     192
 
EaeI y'GGCC_r

Cuts at:      0     140     707    1056 
   Size:        140     567     349

  Fragments arranged by size: 

                 567     349     140
 
EagI C'GGCC_G

Cuts at:      0     707    1056 
   Size:        707     349
 
EarI CTCTTCn'nnn_

Cuts at:      0     626     946    1056 
   Size:        626     320     110

  Fragments arranged by size: 

                 626     320     110
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     244     273    1056 
   Size:        244      29     783

  Fragments arranged by size: 

                 783     244      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     305     477     686    1056 
   Size:        187     118     172     209     370

  Fragments arranged by size: 

                 370     209     187     172     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14      15     879     880    1056 
   Size:         14       1     864       1     176

  Fragments arranged by size: 

                 864     176      14       1       1
 
HaeII r_GCGC'y

Cuts at:      0      38      56     221     463    1056 
   Size:         38      18     165     242     593

  Fragments arranged by size: 

                 593     242     165      38      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     234     265     266     297     625     657     881     913 
   Size:        234      31       1      31     328      32     224      32

Cuts at:    913    1056 
   Size:        143

  Fragments arranged by size: 

                 328     234     224     143      32      32      31      31
                   1
 
KasI G'GCGC_C

Cuts at:      0     459    1056 
   Size:        459     597
 
MscI TGG'CCA

Cuts at:      0     142    1056 
   Size:        142     914
 
MslI CAynn'nnrTG

Cuts at:      0      88     202     349     724    1056 
   Size:         88     114     147     375     332

  Fragments arranged by size: 

                 375     332     147     114      88
 
MspA1I CmG'CkG

Cuts at:      0     468     696    1056 
   Size:        468     228     360

  Fragments arranged by size: 

                 468     360     228
 
NarI GG'CG_CC

Cuts at:      0     460    1056 
   Size:        460     596
 
NcoI C'CATG_G

Cuts at:      0     350     542     758    1056 
   Size:        350     192     216     298

  Fragments arranged by size: 

                 350     298     216     192
 
PasI CC'CwG_GG

Cuts at:      0     507     568    1056 
   Size:        507      61     488

  Fragments arranged by size: 

                 507     488      61
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     513    1056 
   Size:        513     543
 
PspOMI G'GGCC_C

Cuts at:      0      14     879    1056 
   Size:         14     865     177

  Fragments arranged by size: 

                 865     177      14
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     964     996    1056 
   Size:        964      32      60

  Fragments arranged by size: 

                 964      60      32
 
PstI C_TGCA'G

Cuts at:      0     488     836    1056 
   Size:        488     348     220

  Fragments arranged by size: 

                 488     348     220
 
PvuII CAG'CTG

Cuts at:      0     468     696    1056 
   Size:        468     228     360

  Fragments arranged by size: 

                 468     360     228
 
SbfI CC_TGCA'GG

Cuts at:      0     488    1056 
   Size:        488     568
 
ScaI AGT'ACT

Cuts at:      0    1006    1056 
   Size:       1006      50
 
SfcI C'TryA_G

Cuts at:      0     221     484     832    1056 
   Size:        221     263     348     224

  Fragments arranged by size: 

                 348     263     224     221
 
SfoI GGC'GCC

Cuts at:      0     461    1056 
   Size:        461     595
 
StyI C'CwwG_G

Cuts at:      0     350     542     758    1056 
   Size:        350     192     216     298

  Fragments arranged by size: 

                 350     298     216     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     566    1056 
   Size:        566     490
 
TatI w'GTAC_w

Cuts at:      0    1004    1056 
   Size:       1004      52
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     540    1056 
   Size:        540     516
 
XmnI GAAnn'nnTTC

Cuts at:      0     976    1056 
   Size:        976      80
 
ZraI GAC'GTC

Cuts at:      0     281    1056 
   Size:        281     775
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbeI    BbvCI     BglI     BlpI
 Bme1580I     BplI     BpmI   Bpu10I    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI
   BstAPI    BstYI   Bsu36I     BtgI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MscI     MslI   MspA1I
     NarI     NcoI     PasI    PflMI   PspOMI     PsrI     PstI    PvuII
     SbfI     ScaI     SfcI     SfoI     StyI    TaqII     TatI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BsiWI     BsmI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI
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