Restriction map for pF1KE0352
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BbsI        BclI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraI        DraII       DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MluI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Van91I      XbaI        XhoI        
XmnI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          15  CGCG                 CG^CG
AccIII       1233  TCCGGA               T^CCGGA
AspI         1270  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          108  GGCGCC               G^G[CT][GA]CC
BanII         506  GGGCTC               G[GA]GC[CT]^C
BbeI          108  GGCGCC               GGCGC^C
BbvCI         571  GCTGAGG              CCTCAGC(-5/-2)
BciVI         720  GTATCC               GTATCC(6/5)
BglI          499  GCCCTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         181  AGATCT               A^GATCT
BpmI          578  CTGGAG               CTGGAG(16/14)
BsaWI        1233  TCCGGA               [AT]^CCGG[AT]
BseAI        1233  TCCGGA               T^CCGGA
BseRI          40  GAGGAG               GAGGAG(10/8)
BsmFI        1545  GGGAC                GGGAC(10/14)
Bsp1407I      451  TGTACA               T^GTACA
BspDI        1210  ATCGAT               AT^CGAT
BspEI        1233  TCCGGA               T^CCGGA
BspLU11I      377  ACATGT               A^CATGT
BsrGI         451  TGTACA               T^GTACA
BstAPI        968  GCAAGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          15  CGCG                 CG^CG
CfoI          109  GCGC                 GCG^C
ClaI         1210  ATCGAT               AT^CGAT
DraIII        491  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1308  CCTGGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         108  GGCGCC               [GA]GCGC^[CT]
HhaI          109  GCGC                 GCG^C
HinP1I        109  GCGC                 G^CGC
KasI          108  GGCGCC               G^GCGCC
MfeI         1004  CAATTG               C^AATTG
MroI         1233  TCCGGA               T^CCGGA
MslI          912  CACAGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1004  CAATTG               C^AATTG
MvnI           15  CGCG                 CG^CG
NarI          108  GGCGCC               GG^CGCC
NlaIV         108  GGCGCC               GG[ACGT]^[ACGT]CC
PflFI        1270  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         230  CAGCTG               CAG^CTG
ScaI           80  AGTACT               AGT^ACT
SfoI          108  GGCGCC               GGC^GCC
SmaI           28  CCCGGG               CCC^GGG
SspBI         451  TGTACA               T^GTACA
StuI         1046  AGGCCT               AGG^CCT
Tth111I      1270  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          668  CCAACATGATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          28  CCCGGG               C^CCGGG
XmaI           28  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1009  GTCTAC               GT^[AC][GT]AC
             1322  GTCTAC              
AcsI          157  AAATTC               [GA]^AATT[CT]
              340  GAATTT              
AflIII        377  ACATGT               A^C[GA][CT]GT
              728  ACGTGT              
ApoI          157  AAATTC               [GA]^AATT[CT]
              340  GAATTT              
AvaII         908  GGACC                G^G[AT]CC
             1269  GGACC               
BalI           92  TGGCCA               TGG^CCA
             1282  TGGCCA              
BcgI           17  CGATGCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1149  GCACTTGGATCG        
BstYI         181  AGATCT               [GA]^GATC[CT]
              740  AGATCC              
EarI          757  GAAGAG               CTCTTC(1/4)
              903  GAAGAG              
Ksp632I       757  GAAGAG               CTCTTC(1/4)
              903  GAAGAG              
MflI          181  AGATCT               [GA]^GATC[CT]
              740  AGATCC              
MluNI          92  TGGCCA               TGG^CCA
             1282  TGGCCA              
MscI           92  TGGCCA               TGG^CCA
             1282  TGGCCA              
MseI          697  TTAA                 T^TAA
              734  TTAA                
NspI          140  ACATGC               [GA]CATG^[CT]
              377  ACATGT              
PleI         1547  GACTC                GAGTC(4/5)
              607  GAGTC               
PstI          560  CTGCAG               CTGCA^G
             1042  CTGCAG              
TfiI          838  GATTC                G^A[AT]TC
              883  GAATC               
Tru9I         697  TTAA                 T^TAA
              734  TTAA                
XhoII         181  AGATCT               [GA]^GATC[CT]
              740  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaI        BbvI        BcnI        BmyI        
BsaHI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        Hin1I       
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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