Restriction map for pF1KE0352
 (Linear) (Six-base) MAPSORT of: KIEE0352.seq  Check: 1275  from: 1  to: 1590



KIEE0352.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0    1010    1323    1590 
   Size:       1010     313     267

  Fragments arranged by size: 

                1010     313     267
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     922    1590 
   Size:         67     855     668

  Fragments arranged by size: 

                 855     668      67
 
AflIII A'CryG_T

Cuts at:      0     377     728    1590 
   Size:        377     351     862

  Fragments arranged by size: 

                 862     377     351
 
AleI CACnn'nnGTG

Cuts at:      0     916    1590 
   Size:        916     674
 
ApoI r'AATT_y

Cuts at:      0     157     340    1590 
   Size:        157     183    1250

  Fragments arranged by size: 

                1250     183     157
 
AvaI C'yCGr_G

Cuts at:      0      28     272    1417    1590 
   Size:         28     244    1145     173

  Fragments arranged by size: 

                1145     244     173      28
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1158    1191    1590 
   Size:       1158      33     399

  Fragments arranged by size: 

                1158     399      33
 
BanI G'GyrC_C

Cuts at:      0     108    1590 
   Size:        108    1482
 
BanII G_rGCy'C

Cuts at:      0     510    1590 
   Size:        510    1080
 
BbeI G_GCGC'C

Cuts at:      0     112    1590 
   Size:        112    1478
 
BbvCI CC'TCA_GC

Cuts at:      0     572    1590 
   Size:        572    1018
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       6      40    1138    1172    1590 
   Size:          6      34    1098      34     418

  Fragments arranged by size: 

                1098     418      34      34       6
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     731    1590 
   Size:        731     859
 
BglI GCCn_nnn'nGGC

Cuts at:      0     505    1590 
   Size:        505    1085
 
BglII A'GATC_T

Cuts at:      0     181    1590 
   Size:        181    1409
 
Bme1580I G_kGCm'C

Cuts at:      0     501    1590 
   Size:        501    1089
 
BmgBI CAC'GTC

Cuts at:      0    1244    1590 
   Size:       1244     346
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     714    1590 
   Size:        714     876
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      99    1321    1590 
   Size:         99    1222     269

  Fragments arranged by size: 

                1222     269      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599    1590 
   Size:        599     991
 
Bpu10I CC'TnA_GC

Cuts at:      0     410     572    1590 
   Size:        410     162    1018

  Fragments arranged by size: 

                1018     410     162
 
BsaHI Gr'CG_yC

Cuts at:      0      71     109     348    1590 
   Size:         71      38     239    1242

  Fragments arranged by size: 

                1242     239      71      38
 
BsaWI w'CCGG_w

Cuts at:      0    1233    1590 
   Size:       1233     357
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     572     602     992    1022    1228    1258    1590 
   Size:        572      30     390      30     206      30     332

  Fragments arranged by size: 

                 572     390     332     206      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55    1590 
   Size:         55    1535
 
BseYI C'CCAG_C

Cuts at:      0     802    1590 
   Size:        802     788
 
BsiHKAI G_wGCw'C

Cuts at:      0     411    1041    1151    1590 
   Size:        411     630     110     439

  Fragments arranged by size: 

                 630     439     411     110
 
Bsp1286I G_dGCh'C

Cuts at:      0     411     501     510    1041    1151    1590 
   Size:        411      90       9     531     110     439

  Fragments arranged by size: 

                 531     439     411     110      90       9
 
BspEI T'CCGG_A

Cuts at:      0    1233    1590 
   Size:       1233     357
 
BsrGI T'GTAC_A

Cuts at:      0     451    1590 
   Size:        451    1139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     974    1590 
   Size:        974     616
 
BstYI r'GATC_y

Cuts at:      0     181     740    1590 
   Size:        181     559     850

  Fragments arranged by size: 

                 850     559     181
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      31    1590 
   Size:         31    1559
 
BtsI GCAGTG_nn'

Cuts at:      0     593    1590 
   Size:        593     997
 
ClaI AT'CG_AT

Cuts at:      0    1211    1590 
   Size:       1211     379
 
DraIII CAC_nnn'GTG

Cuts at:      0     496    1590 
   Size:        496    1094
 
EaeI y'GGCC_r

Cuts at:      0      92     174     821    1282    1590 
   Size:         92      82     647     461     308

  Fragments arranged by size: 

                 647     461     308      92      82
 
EarI CTCTTCn'nnn_

Cuts at:      0     752     898    1590 
   Size:        752     146     692

  Fragments arranged by size: 

                 752     692     146
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     599     922    1590 
   Size:         67     532     323     668

  Fragments arranged by size: 

                 668     532     323      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1312    1590 
   Size:       1312     278
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     102    1590 
   Size:        102    1488
 
HaeII r_GCGC'y

Cuts at:      0     112    1590 
   Size:        112    1478
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     992    1024    1590 
   Size:        992      32     566

  Fragments arranged by size: 

                 992     566      32
 
KasI G'GCGC_C

Cuts at:      0     108    1590 
   Size:        108    1482
 
MfeI C'AATT_G

Cuts at:      0    1004    1590 
   Size:       1004     586
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1223    1590 
   Size:       1223     367
 
MscI TGG'CCA

Cuts at:      0      94    1284    1590 
   Size:         94    1190     306

  Fragments arranged by size: 

                1190     306      94
 
MslI CAynn'nnrTG

Cuts at:      0     916    1590 
   Size:        916     674
 
MspA1I CmG'CkG

Cuts at:      0      24     232     475     518     641    1590 
   Size:         24     208     243      43     123     949

  Fragments arranged by size: 

                 949     243     208     123      43      24
 
NarI GG'CG_CC

Cuts at:      0     109    1590 
   Size:        109    1481
 
NspI r_CATG'y

Cuts at:      0     144     381    1590 
   Size:        144     237    1209

  Fragments arranged by size: 

                1209     237     144
 
PasI CC'CwG_GG

Cuts at:      0     501    1590 
   Size:        501    1089
 
PciI A'CATG_T

Cuts at:      0     377    1590 
   Size:        377    1213
 
PfoI T'CCnGG_A

Cuts at:      0     677    1590 
   Size:        677     913
 
PsiI TTA'TAA

Cuts at:      0     713    1590 
   Size:        713     877
 
PstI C_TGCA'G

Cuts at:      0     564    1046    1590 
   Size:        564     482     544

  Fragments arranged by size: 

                 564     544     482
 
PvuII CAG'CTG

Cuts at:      0     232    1590 
   Size:        232    1358
 
ScaI AGT'ACT

Cuts at:      0      82    1590 
   Size:         82    1508
 
SfcI C'TryA_G

Cuts at:      0     222     560    1042    1590 
   Size:        222     338     482     548

  Fragments arranged by size: 

                 548     482     338     222
 
SfoI GGC'GCC

Cuts at:      0     110    1590 
   Size:        110    1480
 
SmaI CCC'GGG

Cuts at:      0      30    1590 
   Size:         30    1560
 
StuI AGG'CCT

Cuts at:      0    1048    1590 
   Size:       1048     542
 
StyI C'CwwG_G

Cuts at:      0      56     104     296     425    1590 
   Size:         56      48     192     129    1165

  Fragments arranged by size: 

                1165     192     129      56      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     647    1590 
   Size:        647     943
 
TatI w'GTAC_w

Cuts at:      0      80     451     869    1442    1590 
   Size:         80     371     418     573     148

  Fragments arranged by size: 

                 573     418     371     148      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     572     604     798     830    1228    1260    1590 
   Size:        572      32     194      32     398      32     330

  Fragments arranged by size: 

                 572     398     330     194      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1273    1590 
   Size:       1273     317
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     675    1590 
   Size:        675     915
 
XmaI C'CCGG_G

Cuts at:      0      28    1590 
   Size:         28    1562
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     ApoI     AvaI     BaeI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BglI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BsrGI   BstAPI    BstYI
    BtgZI     BtsI     ClaI   DraIII     EaeI     EarI  Eco57MI    EcoNI
     FalI    HaeII    Hin4I     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NspI     PasI     PciI     PfoI     PsiI     PstI
    PvuII     ScaI     SfcI     SfoI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII    BamHI     BbsI     BclI    BfrBI     BlpI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    CspCI     DraI     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MluI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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