Restriction map for pF1KB6865
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        MaeI        MfeI        MluI        
MluNI       MscI        MslI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        344  TCCGGA               T^CCGGA
AcsI          517  AAATTT               [GA]^AATT[CT]
AcyI           25  GACGCC               G[GA]^CG[CT]C
AgeI          456  ACCGGT               A^CCGGT
ApoI          517  AAATTT               [GA]^AATT[CT]
AspHI         106  GTGCTC               G[AT]GC[AT]^C
AvaII         333  GGACC                G^G[AT]CC
BamHI         341  GGATCC               G^GATCC
BcgI          428  CGAAGGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI           71  GCCCAGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          106  GTGCTC               G[AGT]GC[ACT]^C
BpmI           82  CTGGAG               CTGGAG(16/14)
BsaAI         511  TACGTG               [CT]AC^GT[GA]
BsaHI          25  GACGCC               G[GA]^CG[CT]C
BseAI         344  TCCGGA               T^CCGGA
BseRI         256  GAGGAG               GAGGAG(10/8)
BsgI            4  GTGCAG               GTGCAG(16/14)
BsiEI          66  CGGCCG               CG[GA][CT]^CG
BsiHKAI       106  GTGCTC               G[AT]GC[AT]^C
BsmAI         365  GAGAC                GTCTC(1/5)
BsmFI         323  GGGAC                GGGAC(10/14)
Bsp1286I      106  GTGCTC               G[AGT]GC[ACT]^C
BspEI         344  TCCGGA               T^CCGGA
EaeI           66  CGGCCG               [CT]^GGCC[GA]
EagI           66  CGGCCG               C^GGCCG
EarI          167  GAAGAG               CTCTTC(1/4)
EclXI          66  CGGCCG               C^GGCCG
Eco52I         66  CGGCCG               C^GGCCG
Hin1I          25  GACGCC               G[GA]^CG[CT]C
Ksp632I       167  GAAGAG               CTCTTC(1/4)
MaeIII        373  GTGAC                ^GT[ACGT]AC
MroI          344  TCCGGA               T^CCGGA
MseI          242  TTAA                 T^TAA
NdeI          204  CATATG               CA^TATG
NlaIV         341  GGATCC               GG[ACGT]^[ACGT]CC
PinAI         456  ACCGGT               A^CCGGT
PvuII          88  CAGCTG               CAG^CTG
SfaNI         124  GATGC                GCATC(5/9)
SmaI           30  CCCGGG               CCC^GGG
StuI            8  AGGCCT               AGG^CCT
TaqI          314  TCGA                 T^CGA
Tru9I         242  TTAA                 T^TAA
Tsp45I        373  GTGAC                ^GT[GC]AC
TthHB8I       314  TCGA                 T^CGA
XmaCI          30  CCCGGG               C^CCGGG
XmaI           30  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI           89  AGCT                 AG^CT
              119  AGCT                
AvaI           30  CCCGGG               C^[CT]CG[GA]G
              100  CTCGGG              
BbvI           87  GCAGC                GCAGC(8/12)
               90  GCTGC               
BsaBI         189  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              342  GATCCGGATC          
BsaWI         344  TCCGGA               [AT]^CCGG[AT]
              456  ACCGGT              
BsoBI          30  CCCGGG               C^[CT]CG[GA]G
              100  CTCGGG              
BsrFI         456  ACCGGT               [GA]^CCGG[CT]
              476  ACCGGC              
Cfr10I        456  ACCGGT               [GA]^CCGG[CT]
              476  ACCGGC              
HgaI           25  GACGC                GACGC(5/10)
              375  GACGC               
HphI          372  GGTGA                GGTGA(8/7)
              396  TCACC               
MaeII         317  ACGT                 A^CGT
              512  ACGT                
MamI          189  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              342  GATCCGGATC          
TseI           87  GCAGC                G^C[AT]GC
               90  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AfaI        AlwI        BcnI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
Cfr13I      DpnII       DsaI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        KspI        MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      RsaI        
SacII       Sau3AI      Sau96I      ScrFI       Tsp509I     
TspRI       XhoII       
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