Restriction map for pF1KB6865
 (Linear) (Six-base) MAPSORT of: KIBB6865.seq  Check: 439  from: 1  to: 537



KIBB6865.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AgeI A'CCGG_T

Cuts at:      0     456     537 
   Size:        456      81
 
ApoI r'AATT_y

Cuts at:      0     517     537 
   Size:        517      20
 
AvaI C'yCGr_G

Cuts at:      0      30     100     537 
   Size:         30      70     437

  Fragments arranged by size: 

                 437      70      30
 
BamHI G'GATC_C

Cuts at:      0     341     537 
   Size:        341     196
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     417     451     537 
   Size:        417      34      86

  Fragments arranged by size: 

                 417      86      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      77     537 
   Size:         77     460
 
BmgBI CAC'GTC

Cuts at:      0     318     537 
   Size:        318     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      67     537 
   Size:         67     470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     537 
   Size:        103     434
 
BsaAI yAC'GTr

Cuts at:      0     513     537 
   Size:        513      24
 
BsaBI GATnn'nnATC

Cuts at:      0     193     346     537 
   Size:        193     153     191

  Fragments arranged by size: 

                 193     191     153
 
BsaHI Gr'CG_yC

Cuts at:      0      26     537 
   Size:         26     511
 
BsaWI w'CCGG_w

Cuts at:      0     344     456     537 
   Size:        344     112      81

  Fragments arranged by size: 

                 344     112      81
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352     356     386     443     473     537 
   Size:        322      30       4      30      57      30      64

  Fragments arranged by size: 

                 322      64      57      30      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     537 
   Size:        271     266
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     537 
   Size:         25     512
 
BsiEI CG_ry'CG

Cuts at:      0      69     537 
   Size:         69     468
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     537 
   Size:        110     427
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     537 
   Size:        110     427
 
BspEI T'CCGG_A

Cuts at:      0     344     537 
   Size:        344     193
 
BsrFI r'CCGG_y

Cuts at:      0     456     476     537 
   Size:        456      20      61

  Fragments arranged by size: 

                 456      61      20
 
BstYI r'GATC_y

Cuts at:      0     161     281     341     347     389     537 
   Size:        161     120      60       6      42     148

  Fragments arranged by size: 

                 161     148     120      60      42       6
 
BtgI C'CryG_G

Cuts at:      0      40     383     501     537 
   Size:         40     343     118      36

  Fragments arranged by size: 

                 343     118      40      36
 
BtsI GCAGTG_nn'

Cuts at:      0     309     537 
   Size:        309     228
 
EaeI y'GGCC_r

Cuts at:      0      66     537 
   Size:         66     471
 
EagI C'GGCC_G

Cuts at:      0      66     537 
   Size:         66     471
 
EarI CTCTTCn'nnn_

Cuts at:      0     162     537 
   Size:        162     375
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     537 
   Size:        103     434
 
MspA1I CmG'CkG

Cuts at:      0      42      90     385     484     503     537 
   Size:         42      48     295      99      19      34

  Fragments arranged by size: 

                 295      99      48      42      34      19
 
NdeI CA'TA_TG

Cuts at:      0     205     537 
   Size:        205     332
 
PfoI T'CCnGG_A

Cuts at:      0     184     293     537 
   Size:        184     109     244

  Fragments arranged by size: 

                 244     184     109
 
PvuII CAG'CTG

Cuts at:      0      90     537 
   Size:         90     447
 
SacII CC_GC'GG

Cuts at:      0      43     386     504     537 
   Size:         43     343     118      33

  Fragments arranged by size: 

                 343     118      43      33
 
SmaI CCC'GGG

Cuts at:      0      32     537 
   Size:         32     505
 
StuI AGG'CCT

Cuts at:      0      10     537 
   Size:         10     527
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     322     354     356     388     443     475     537 
   Size:        322      32       2      32      55      32      62

  Fragments arranged by size: 

                 322      62      55      32      32      32       2
 
XmaI C'CCGG_G

Cuts at:      0      30     537 
   Size:         30     507
 

 Enzymes that do cut:

     AgeI     ApoI     AvaI    BamHI     BcgI     BglI    BmgBI     BmrI
     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BsrFI    BstYI     BtgI     BtsI
     EaeI     EagI     EarI  Eco57MI   MspA1I     NdeI     PfoI    PvuII
    SacII     SmaI     StuI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BseYI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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