Restriction map for pF1KB7288
 (Linear) (Six-base) MAPSORT of: KIBB7288.seq  Check: 2190  from: 1  to: 3633



KIBB7288.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     371    1804    2258    2796    3633 
   Size:        371    1433     454     538     837

  Fragments arranged by size: 

                1433     837     538     454     371
 
AccI GT'mk_AC

Cuts at:      0    2267    2528    2564    3633 
   Size:       2267     261      36    1069

  Fragments arranged by size: 

                2267    1069     261      36
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2040    2599    3633 
   Size:       2040     559    1034

  Fragments arranged by size: 

                2040    1034     559
 
AflIII A'CryG_T

Cuts at:      0    3350    3633 
   Size:       3350     283
 
AleI CACnn'nnGTG

Cuts at:      0     439    1738    3301    3633 
   Size:        439    1299    1563     332

  Fragments arranged by size: 

                1563    1299     439     332
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1151    1964    2824    3633 
   Size:       1151     813     860     809

  Fragments arranged by size: 

                1151     860     813     809
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     547     579    3633 
   Size:        547      32    3054

  Fragments arranged by size: 

                3054     547      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     521     678    1014    1365    2063    2162    3132    3633 
   Size:        521     157     336     351     698      99     970     501

  Fragments arranged by size: 

                 970     698     521     501     351     336     157      99
            
 
ApaI G_GGCC'C

Cuts at:      0     146    1595    2431    2577    2948    3633 
   Size:        146    1449     836     146     371     685

  Fragments arranged by size: 

                1449     836     685     371     146     146
 
ApaLI G'TGCA_C

Cuts at:      0    1506    1616    2860    3633 
   Size:       1506     110    1244     773

  Fragments arranged by size: 

                1506    1244     773     110
 
AvaI C'yCGr_G

Cuts at:      0     282     397     454     661     756    1443    1800    2477 
   Size:        282     115      57     207      95     687     357     677

Cuts at:   2477    2890    3633 
   Size:        413     743

  Fragments arranged by size: 

                 743     687     677     413     357     282     207     115
                  95      57
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     850     883    3633 
   Size:        850      33    2750

  Fragments arranged by size: 

                2750     850      33
 
BamHI G'GATC_C

Cuts at:      0       3    3633 
   Size:          3    3630
 
BanI G'GyrC_C

Cuts at:      0     177     393     430     922    1305    2440    2980    3633 
   Size:        177     216      37     492     383    1135     540     653

  Fragments arranged by size: 

                1135     653     540     492     383     216     177      37
            
 
BanII G_rGCy'C

Cuts at:      0     146     258     449    1595    2431    2577    2921    2948 
   Size:        146     112     191    1146     836     146     344      27

Cuts at:   2948    3556    3593    3633 
   Size:        608      37      40

  Fragments arranged by size: 

                1146     836     608     344     191     146     146     112
                  40      37      27
 
BbeI G_GCGC'C

Cuts at:      0     397    1309    3633 
   Size:        397     912    2324

  Fragments arranged by size: 

                2324     912     397
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2132    3633 
   Size:       2132    1501
 
BbvCI CC'TCA_GC

Cuts at:      0     715    1203    3633 
   Size:        715     488    2430

  Fragments arranged by size: 

                2430     715     488
 
BglI GCCn_nnn'nGGC

Cuts at:      0     150     262    3633 
   Size:        150     112    3371

  Fragments arranged by size: 

                3371     150     112
 
BglII A'GATC_T

Cuts at:      0    3167    3633 
   Size:       3167     466
 
BlpI GC'TnA_GC

Cuts at:      0     514     608    1366    3633 
   Size:        514      94     758    2267

  Fragments arranged by size: 

                2267     758     514      94
 
Bme1580I G_kGCm'C

Cuts at:      0     146     155     182     433     771     927    1510    1595 
   Size:        146       9      27     251     338     156     583      85

Cuts at:   1595    1620    2182    2431    2577    2864    2948    3377    3633 
   Size:         25     562     249     146     287      84     429     256

  Fragments arranged by size: 

                 583     562     429     338     287     256     251     249
                 156     146     146      85      84      27      25       9
            
 
BmgBI CAC'GTC

Cuts at:      0     480     650    2340    3351    3633 
   Size:        480     170    1690    1011     282

  Fragments arranged by size: 

                1690    1011     480     282     170
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2616    2949    3364    3633 
   Size:       2616     333     415     269

  Fragments arranged by size: 

                2616     415     333     269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1036    2097    2323    3423    3439    3633 
   Size:       1027       9    1061     226    1100      16     194

  Fragments arranged by size: 

                1100    1061    1027     226     194      16       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     715     961    1203    3633 
   Size:        715     246     242    2430

  Fragments arranged by size: 

                2430     715     246     242
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     347    1981    2487    2594    3633 
   Size:        347    1634     506     107    1039

  Fragments arranged by size: 

                1634    1039     506     347     107
 
BsaAI yAC'GTr

Cuts at:      0     219     858    1236    1723    3120    3633 
   Size:        219     639     378     487    1397     513

  Fragments arranged by size: 

                1397     639     513     487     378     219
 
BsaHI Gr'CG_yC

Cuts at:      0     194     394     731    1306    1913    2051    2270    2882 
   Size:        194     200     337     575     607     138     219     612

Cuts at:   2882    3633 
   Size:        751

  Fragments arranged by size: 

                 751     612     607     575     337     219     200     194
                 138
 
BsaWI w'CCGG_w

Cuts at:      0     275     545    1161    1517    3161    3633 
   Size:        275     270     616     356    1644     472

  Fragments arranged by size: 

                1644     616     472     356     275     270
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577     639     669    1183    1213    1366    1396 
   Size:        547      30      62      30     514      30     153      30

Cuts at:   1396    1987    2017    2371    2401    3633 
   Size:        591      30     354      30    1232

  Fragments arranged by size: 

                1232     591     547     514     354     153      62      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     322     492     568     571     574     647     754 
   Size:        319       3     170      76       3       3      73     107

Cuts at:    754    1191    1885    3055    3633 
   Size:        437     694    1170     578

  Fragments arranged by size: 

                1170     694     578     437     319     170     107      76
                  73       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     813    1031    1239    1290    1436    1688    1916    2741 
   Size:        813     218     208      51     146     252     228     825

Cuts at:   2741    3633 
   Size:        892

  Fragments arranged by size: 

                 892     825     813     252     228     218     208     146
                  51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     856    1158    1336    1363    1435    1569    3633 
   Size:        343     513     302     178      27      72     134    2064

  Fragments arranged by size: 

                2064     513     343     302     178     134      72      27
            
 
BsiEI CG_ry'CG

Cuts at:      0    2406    3550    3633 
   Size:       2406    1144      83

  Fragments arranged by size: 

                2406    1144      83
 
BsiHKAI G_wGCw'C

Cuts at:      0     746     857    1253    1510    1620    1907    1943    2599 
   Size:        746     111     396     257     110     287      36     656

Cuts at:   2599    2864    2921    2962    3002    3593    3633 
   Size:        265      57      41      40     591      40

  Fragments arranged by size: 

                 746     656     591     396     287     265     257     111
                 110      57      41      40      40      36
 
BsmI GAATG_Cn'

Cuts at:      0    2740    2825    3633 
   Size:       2740      85     808

  Fragments arranged by size: 

                2740     808      85
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     470    2387    2404    3633 
   Size:        470    1917      17    1229

  Fragments arranged by size: 

                1917    1229     470      17
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     155     182     258     433     449     746     771 
   Size:        146       9      27      76     175      16     297      25

Cuts at:    771     857     927    1253    1510    1595    1620    1907    1943 
   Size:         86      70     326     257      85      25     287      36

Cuts at:   1943    2182    2431    2577    2599    2864    2921    2948    2962 
   Size:        239     249     146      22     265      57      27      14

Cuts at:   2962    3002    3377    3556    3593    3633 
   Size:         40     375     179      37      40

  Fragments arranged by size: 

                 375     326     297     287     265     257     249     239
                 179     175     146     146      86      85      76      70
                  57      40      40      37      36      27      27      25
                  25      22      16      14       9
 
BspEI T'CCGG_A

Cuts at:      0     275     545    1517    3161    3633 
   Size:        275     270     972    1644     472

  Fragments arranged by size: 

                1644     972     472     275     270
 
BspHI T'CATG_A

Cuts at:      0     881    3633 
   Size:        881    2752
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     365     371     878    1385    1804    2258    2796    3073 
   Size:        365       6     507     507     419     454     538     277

Cuts at:   3073    3633 
   Size:        560

  Fragments arranged by size: 

                 560     538     507     507     454     419     365     277
                   6
 
BsrBI CCG'CTC

Cuts at:      0      22      78     160    2855    2991    3633 
   Size:         22      56      82    2695     136     642

  Fragments arranged by size: 

                2695     642     136      82      56      22
 
BsrDI GCAATG_nn'

Cuts at:      0    1477    2363    2883    3633 
   Size:       1477     886     520     750

  Fragments arranged by size: 

                1477     886     750     520
 
BsrFI r'CCGG_y

Cuts at:      0       9     484     638    1147    1425    3633 
   Size:          9     475     154     509     278    2208

  Fragments arranged by size: 

                2208     509     475     278     154       9
 
BsrGI T'GTAC_A

Cuts at:      0    3284    3633 
   Size:       3284     349
 
BssSI C'ACGA_G

Cuts at:      0     388    1936    3633 
   Size:        388    1548    1697

  Fragments arranged by size: 

                1697    1548     388
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2720    3633 
   Size:       2720     913
 
BstEII G'GTnAC_C

Cuts at:      0    2192    3633 
   Size:       2192    1441
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     441    1228    2376    2788    3633 
   Size:        441     787    1148     412     845

  Fragments arranged by size: 

                1148     845     787     441     412
 
BstYI r'GATC_y

Cuts at:      0       3    2228    3099    3167    3633 
   Size:          3    2225     871      68     466

  Fragments arranged by size: 

                2225     871     466      68       3
 
BtgI C'CryG_G

Cuts at:      0     843    2369    2423    2436    2673    3011    3386    3501 
   Size:        843    1526      54      13     237     338     375     115

Cuts at:   3501    3633 
   Size:        132

  Fragments arranged by size: 

                1526     843     375     338     237     132     115      54
                  13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     539    3633 
   Size:        539    3094
 
BtsI GCAGTG_nn'

Cuts at:      0    1644    1882    2160    2871    3183    3633 
   Size:       1644     238     278     711     312     450

  Fragments arranged by size: 

                1644     711     450     312     278     238
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1254    1289    2589    2624    3633 
   Size:       1254      35    1300      35    1009

  Fragments arranged by size: 

                1300    1254    1009      35      35
 
DraI TTT'AAA

Cuts at:      0    1638    3633 
   Size:       1638    1995
 
DraIII CAC_nnn'GTG

Cuts at:      0     222    3633 
   Size:        222    3411
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2479    2539    2560    3633 
   Size:       2479      60      21    1073

  Fragments arranged by size: 

                2479    1073      60      21
 
EaeI y'GGCC_r

Cuts at:      0     348     482     640    1230    1423    1778    2288    2403 
   Size:        348     134     158     590     193     355     510     115

Cuts at:   2403    2992    3397    3633 
   Size:        589     405     236

  Fragments arranged by size: 

                 590     589     510     405     355     348     236     193
                 158     134     115
 
EagI C'GGCC_G

Cuts at:      0    2403    3633 
   Size:       2403    1230
 
EarI CTCTTCn'nnn_

Cuts at:      0     331    2324    2543    3106    3633 
   Size:        331    1993     219     563     527

  Fragments arranged by size: 

                1993     563     527     331     219
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     777    2627    2808    3633 
   Size:        777    1850     181     825

  Fragments arranged by size: 

                1850     825     777     181
 
EcoICRI GAG'CTC

Cuts at:      0    2919    3591    3633 
   Size:       2919     672      42

  Fragments arranged by size: 

                2919     672      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1027    1036    2040    2097    2323    2599    3423    3439 
   Size:       1027       9    1004      57     226     276     824      16

Cuts at:   3439    3633 
   Size:        194

  Fragments arranged by size: 

                1027    1004     824     276     226     194      57      16
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1315    2548    3633 
   Size:       1315    1233    1085

  Fragments arranged by size: 

                1315    1233    1085
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     675     689     807    1591    1592    1751    1890 
   Size:        143     532      14     118     784       1     159     139

Cuts at:   1890    2428    2574    2618    2945    3062    3423    3633 
   Size:        538     146      44     327     117     361     210

  Fragments arranged by size: 

                 784     538     532     361     327     210     159     146
                 143     139     118     117      44      14       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2070    2337    3633 
   Size:       2070     267    1296

  Fragments arranged by size: 

                2070    1296     267
 
FseI GG_CCGG'CC

Cuts at:      0     488    3633 
   Size:        488    3145
 
FspI TGC'GCA

Cuts at:      0     187    3633 
   Size:        187    3446
 
FspAI rTGC'GCAy

Cuts at:      0     187    3633 
   Size:        187    3446
 
HaeII r_GCGC'y

Cuts at:      0     397     514    1309    1432    1697    3633 
   Size:        397     117     795     123     265    1936

  Fragments arranged by size: 

                1936     795     397     265     123     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1183    1215    1634    1666    1987    2019    2237    2269 
   Size:       1183      32     419      32     321      32     218      32

Cuts at:   2269    3136    3168    3633 
   Size:        867      32     465

  Fragments arranged by size: 

                1183     867     465     419     321     218      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2529    3633 
   Size:       2529    1104
 
KasI G'GCGC_C

Cuts at:      0     393    1305    3633 
   Size:        393     912    2328

  Fragments arranged by size: 

                2328     912     393
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     453    3633 
   Size:        453    3180
 
MscI TGG'CCA

Cuts at:      0    1232    3633 
   Size:       1232    2401
 
MslI CAynn'nnrTG

Cuts at:      0     439     934    1150    1385    1738    1946    2218    2368 
   Size:        439     495     216     235     353     208     272     150

Cuts at:   2368    2374    2464    2733    2806    2876    3301    3349    3391 
   Size:          6      90     269      73      70     425      48      42

Cuts at:   3391    3633 
   Size:        242

  Fragments arranged by size: 

                 495     439     425     353     272     269     242     235
                 216     208     150      90      73      70      48      42
                   6
 
MspA1I CmG'CkG

Cuts at:      0    1031    1172    1271    1349    1542    1688    3013    3633 
   Size:       1031     141      99      78     193     146    1325     620

  Fragments arranged by size: 

                1325    1031     620     193     146     141      99      78
            
 
NaeI GCC'GGC

Cuts at:      0      11     486     640    1427    3633 
   Size:         11     475     154     787    2206

  Fragments arranged by size: 

                2206     787     475     154      11
 
NarI GG'CG_CC

Cuts at:      0     394    1306    3633 
   Size:        394     912    2327

  Fragments arranged by size: 

                2327     912     394
 
NcoI C'CATG_G

Cuts at:      0    2369    2423    3386    3501    3633 
   Size:       2369      54     963     115     132

  Fragments arranged by size: 

                2369     963     132     115      54
 
NgoMIV G'CCGG_C

Cuts at:      0       9     484     638    1425    3633 
   Size:          9     475     154     787    2208

  Fragments arranged by size: 

                2208     787     475     154       9
 
NspI r_CATG'y

Cuts at:      0     628     781    1128    1155    2805    3633 
   Size:        628     153     347      27    1650     828

  Fragments arranged by size: 

                1650     828     628     347     153      27
 
PasI CC'CwG_GG

Cuts at:      0     147     198     726    3633 
   Size:        147      51     528    2907

  Fragments arranged by size: 

                2907     528     147      51
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1239    2286    2664    3328    3507    3633 
   Size:       1239    1047     378     664     179     126

  Fragments arranged by size: 

                1239    1047     664     378     179     126
 
PfoI T'CCnGG_A

Cuts at:      0    2543    3633 
   Size:       2543    1090
 
PmlI CAC'GTG

Cuts at:      0     219    1236    3633 
   Size:        219    1017    2397

  Fragments arranged by size: 

                2397    1017     219
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     547     579    3633 
   Size:        547      32    3054

  Fragments arranged by size: 

                3054     547      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     807    1751    1890    2618    3062    3423    3633 
   Size:        807     944     139     728     444     361     210

  Fragments arranged by size: 

                 944     807     728     444     361     210     139
 
PspOMI G'GGCC_C

Cuts at:      0     142    1591    2427    2573    2944    3633 
   Size:        142    1449     836     146     371     689

  Fragments arranged by size: 

                1449     836     689     371     146     142
 
PspXI vC'TCGA_Gb

Cuts at:      0     756    3633 
   Size:        756    2877
 
PstI C_TGCA'G

Cuts at:      0    1354    1831    2083    2238    2420    2911    3070    3633 
   Size:       1354     477     252     155     182     491     159     563

  Fragments arranged by size: 

                1354     563     491     477     252     182     159     155
            
 
PvuII CAG'CTG

Cuts at:      0    1172    1542    1688    3633 
   Size:       1172     370     146    1945

  Fragments arranged by size: 

                1945    1172     370     146
 
RsrII CG'GwC_CG

Cuts at:      0     273    1164    3633 
   Size:        273     891    2469

  Fragments arranged by size: 

                2469     891     273
 
SacI G_AGCT'C

Cuts at:      0    2921    3593    3633 
   Size:       2921     672      40

  Fragments arranged by size: 

                2921     672      40
 
SacII CC_GC'GG

Cuts at:      0    3014    3633 
   Size:       3014     619
 
SalI G'TCGA_C

Cuts at:      0    2527    3633 
   Size:       2527    1106
 
SanDI GG'GwC_CC

Cuts at:      0    2618    3633 
   Size:       2618    1015
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3106    3633 
   Size:       3106     527
 
SbfI CC_TGCA'GG

Cuts at:      0    2238    3070    3633 
   Size:       2238     832     563

  Fragments arranged by size: 

                2238     832     563
 
SfcI C'TryA_G

Cuts at:      0    1350    1827    2079    2234    2416    2907    3066    3210 
   Size:       1350     477     252     155     182     491     159     144

Cuts at:   3210    3380    3633 
   Size:        170     253

  Fragments arranged by size: 

                1350     491     477     253     252     182     170     159
                 155     144
 
SfoI GGC'GCC

Cuts at:      0     395    1307    3633 
   Size:        395     912    2326

  Fragments arranged by size: 

                2326     912     395
 
SgrAI Cr'CCGG_yG

Cuts at:      0       9    3633 
   Size:          9    3624
 
SmaI CCC'GGG

Cuts at:      0     399    1445    2479    3633 
   Size:        399    1046    1034    1154

  Fragments arranged by size: 

                1154    1046    1034     399
 
SmlI C'TyrA_G

Cuts at:      0     756    3633 
   Size:        756    2877
 
SnaBI TAC'GTA

Cuts at:      0    3120    3633 
   Size:       3120     513
 
SphI G_CATG'C

Cuts at:      0     781    1128    2805    3633 
   Size:        781     347    1677     828

  Fragments arranged by size: 

                1677     828     781     347
 
SspI AAT'ATT

Cuts at:      0    3206    3633 
   Size:       3206     427
 
StyI C'CwwG_G

Cuts at:      0     578     917    1598    1952    2369    2423    3189    3386 
   Size:        578     339     681     354     417      54     766     197

Cuts at:   3386    3501    3633 
   Size:        115     132

  Fragments arranged by size: 

                 766     681     578     417     354     339     197     132
                 115      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     660     910    2211    2998    3479    3543    3564    3633 
   Size:        660     250    1301     787     481      64      21      69

  Fragments arranged by size: 

                1301     787     660     481     250      69      64      21
            
 
TatI w'GTAC_w

Cuts at:      0    1895    1970    2387    3284    3633 
   Size:       1895      75     417     897     349

  Fragments arranged by size: 

                1895     897     417     349      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     639     671    1599    1631    1652    1684    2116    2148 
   Size:        639      32     928      32      21      32     432      32

Cuts at:   2148    3633 
   Size:       1485

  Fragments arranged by size: 

                1485     928     639     432      32      32      32      32
                  21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2013    2283    2451    3384    3633 
   Size:       2013     270     168     933     249

  Fragments arranged by size: 

                2013     933     270     249     168
 
XhoI C'TCGA_G

Cuts at:      0     756    3633 
   Size:        756    2877
 
XmaI C'CCGG_G

Cuts at:      0     397    1443    2477    3633 
   Size:        397    1046    1034    1156

  Fragments arranged by size: 

                1156    1046    1034     397
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI    ApaLI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfcI     SfoI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SspI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     ApoI
     AscI     AseI    AsiSI    AvrII     BcgI    BciVI     BclI    BfrBI
     BmtI     BplI    BpuEI    BsaBI    BsiWI   BssHII    BstBI  BstZ17I
   Bsu36I     ClaI    EcoRI    EcoRV  HindIII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI    PshAI     PsiI     PsrI     PvuI     ScaI    SexAI     SfiI
     SgfI     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XmnI
     ZraI
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