Restriction map for pF1KB6579
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BpmI        BsaWI       BseAI       BsgI        
BsiWI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DsaI        
Eam1105I    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Aor51HI       764  AGCGCT               AGC^GCT
AspI          475  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          298  CCCGAG               C^[CT]CG[GA]G
AvrII          85  CCTAGG               C^CTAGG
BalI          407  TGGCCA               TGG^CCA
BbsI           10  GAAGAC               GAAGAC(2/6)
BciVI         194  GTATCC               GTATCC(6/5)
BfaI           86  CTAG                 C^TAG
BglII         257  AGATCT               A^GATCT
BlnI           85  CCTAGG               C^CTAGG
BlpI          616  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      616  GCTGAGC              GC^T[ACGT]AGC
BpuAI          10  GAAGAC               GAAGAC(2/6)
BsiEI         692  CGGCCG               CG[GA][CT]^CG
BsmBI         781  GAGACG               CGTCTC(1/5)
BsmFI         426  GTCCC                GGGAC(10/14)
BsmI          824  GCATTC               GAATGC(1/-1)
BsoBI         298  CCCGAG               C^[CT]CG[GA]G
BspHI         851  TCATGA               T^CATGA
BspMI         741  GCAGGT               ACCTGC(4/8)
BsrI          239  ACTGG                ACTGG(1/-1)
BstXI         398  CCATGTACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         616  GCTGAGC              GC^T[ACGT]AGC
DraIII        487  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          736  GACGGGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          692  CGGCCG               C^GGCCG
EarI          787  CTCTTC               CTCTTC(1/4)
EclXI         692  CGGCCG               C^GGCCG
Eco47III      764  AGCGCT               AGC^GCT
Eco52I        692  CGGCCG               C^GGCCG
EcoNI         437  CCTCCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI         1098  GGCCGGCC             GGCCGG^CC
HaeII         764  AGCGCT               [GA]GCGC^[CT]
Ksp632I       787  CTCTTC               CTCTTC(1/4)
MaeI           86  CTAG                 C^TAG
MluNI         407  TGGCCA               TGG^CCA
MscI          407  TGGCCA               TGG^CCA
NspI          941  GCATGC               [GA]CATG^[CT]
PflFI         475  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         878  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         614  CAGCTG               CAG^CTG
RcaI          851  TCATGA               T^CATGA
SapI          786  GCTCTTC              GCTCTTC(1/4)
SfiI          408  GGCCATCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          941  GCATGC               GCATG^C
TfiI          157  GATTC                G^A[AT]TC
TspRI         585  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       475  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          527  CCATCATGCGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI         220  GAGCAC               G[AT]GC[AT]^C
              304  GAGCAC              
BcnI           71  CCGGG                CC^[GC]GG
              777  CCCGG               
BsiHKAI       220  GAGCAC               G[AT]GC[AT]^C
              304  GAGCAC              
BssSI         829  CACGAG               CACGAG(-5/-1)
               28  CTCGTG              
BstYI         257  AGATCT               [GA]^GATC[CT]
              578  AGATCC              
DraII         326  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              878  AGGACCT             
EaeI          407  TGGCCA               [CT]^GGCC[GA]
              692  CGGCCG              
EcoO109I      326  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              878  AGGACCT             
EcoT14I        85  CCTAGG               C^C[AT][AT]GG
              341  CCAAGG              
MflI          257  AGATCT               [GA]^GATC[CT]
              578  AGATCC              
MspA1I        614  CAGCTG               C[AC]G^C[GT]G
              771  CCGCTG              
NaeI           61  GCCGGC               GCC^GGC
             1099  GCCGGC              
NciI           71  CCGGG                CC^[GC]GG
              777  CCCGG               
NgoMIV         61  GCCGGC               G^CCGGC
             1099  GCCGGC              
PleI          466  GACTC                GAGTC(4/5)
              814  GAGTC               
StyI           85  CCTAGG               C^C[AT][AT]GG
              341  CCAAGG              
XhoII         257  AGATCT               [GA]^GATC[CT]
              578  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BanI        BbvI        BglI        
BmyI        BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrFI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MamI        MboI        
MboII       MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        Tsp45I      TthHB8I     
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