Restriction map for pF1KB7223
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        TaqI        Tth111I     TthHB8I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII        594  ACATGT               A^C[GA][CT]GT
AlwI          755  GGATC                GGATC(4/5)
AlwNI         356  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       342  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         187  GTGCTC               G[AT]GC[AT]^C
BalI          268  TGGCCA               TGG^CCA
BanI          786  GGTGCC               G^G[CT][GA]CC
BanII         210  GGGCTC               G[GA]GC[CT]^C
BclI         1370  TGATCA               T^GATCA
BcnI         1146  CCCGG                CC^[GC]GG
BsaBI        1371  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         167  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       187  GTGCTC               G[AT]GC[AT]^C
Bsp1407I     1090  TGTACA               T^GTACA
BspLU11I      594  ACATGT               A^CATGT
BsrDI         978  GCAATG               GCAATG(2/0)
BsrGI        1090  TGTACA               T^GTACA
BstEII        840  GGTAACC              G^GT[ACGT]ACC
BstNI        1006  CCTGG                CC^[AT]GG
BstPI         840  GGTAACC              G^GT[ACGT]ACC
Cfr13I       1361  GGGCC                G^G[ACGT]CC
EcoO65I       840  GGTAACC              G^GT[ACGT]ACC
EcoRII       1006  CCTGG                ^CC[AT]GG
EcoRV        1443  GATATC               GAT^ATC
EcoT22I      1422  ATGCAT               ATGCA^T
FbaI         1370  TGATCA               T^GATCA
MaeII         280  ACGT                 A^CGT
MamI         1371  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         268  TGGCCA               TGG^CCA
MscI          268  TGGCCA               TGG^CCA
MvaI         1006  CCTGG                CC^[AT]GG
NciI         1146  CCCGG                CC^[GC]GG
NsiI         1422  ATGCAT               ATGCA^T
NspI          594  ACATGT               [GA]CATG^[CT]
PflMI         730  CCAGCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          910  CTGCAG               CTGCA^G
Sau96I       1361  GGGCC                G^G[ACGT]CC
ScaI         1037  AGTACT               AGT^ACT
SspBI        1090  TGTACA               T^GTACA
SspI          154  AATATT               AAT^ATT
Van91I        730  CCAGCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          451  TCTAGA               T^CTAGA
XmnI          342  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI          849  CCGC                 CCGC(-3/-1)
             1303  GCGG                
BbsI          858  GAAGAC               GAAGAC(2/6)
              598  GTCTTC              
BciVI         146  GGATAC               GTATCC(6/5)
              403  GTATCC              
BfaI          452  CTAG                 C^TAG
             1112  CTAG                
BglII         236  AGATCT               A^GATCT
             1329  AGATCT              
BmyI          187  GTGCTC               G[AGT]GC[ACT]^C
              210  GGGCTC              
BpuAI         858  GAAGAC               GAAGAC(2/6)
              598  GTCTTC              
BsaJI         735  CCTTGG               C^C[ACGT][ACGT]GG
              880  CCTTGG              
BseRI        1466  CTCCTC               GAGGAG(10/8)
             1507  GAGGAG              
BsmAI         217  GTCTC                GTCTC(1/5)
              670  GTCTC               
Bsp1286I      187  GTGCTC               G[AGT]GC[ACT]^C
              210  GGGCTC              
BssKI        1006  CCTGG                ^CC[ACGT]GG
             1146  CCCGG               
BstF5I        846  CATCC                GGATG(2/0)
              985  GGATG               
BstYI         236  AGATCT               [GA]^GATC[CT]
             1329  AGATCT              
Cac8I         248  GCTTGC               GC[ACGT]^[ACGT]GC
              831  GCACGC              
EaeI          268  TGGCCA               [CT]^GGCC[GA]
             1148  CGGCCA              
EarI          694  CTCTTC               CTCTTC(1/4)
              677  GAAGAG              
EcoT14I       735  CCTTGG               C^C[AT][AT]GG
              880  CCTTGG              
FokI          846  CATCC                GGATG(9/13)
              985  GGATG               
HindIII        58  AAGCTT               A^AGCTT
             1184  AAGCTT              
Ksp632I       694  CTCTTC               CTCTTC(1/4)
              677  GAAGAG              
MaeI          452  CTAG                 C^TAG
             1112  CTAG                
MflI          236  AGATCT               [GA]^GATC[CT]
             1329  AGATCT              
MwoI          173  GCAAAATTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              923  GCAGCTACAGC         
PleI           41  GACTC                GAGTC(4/5)
             1464  GACTC               
ScrFI        1006  CCTGG                CC^[ACGT]GG
             1146  CCCGG               
SfaNI         358  GATGC                GCATC(5/9)
              748  GATGC               
StyI          735  CCTTGG               C^C[AT][AT]GG
              880  CCTTGG              
XhoII         236  AGATCT               [GA]^GATC[CT]
             1329  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BsiYI       BslI        BsmI        BsrI        DdeI        
DpnII       Fnu4HI      HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
NdeII       NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      SfcI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com