Restriction map for pF1KA1996
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# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwI        AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BfaI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   54 enzymes) #
###################################

AclI         1170  AACGTT               AA^CGTT
AfaI          712  GTAC                 GT^AC
AhdI          958  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       944  AGCGCT               AGC^GCT
Asp700I       700  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         958  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          968  CCCGAG               C^[CT]CG[GA]G
BanII         951  GGGCTC               G[GA]GC[CT]^C
BbvCI        1111  CCTCAGC              CCTCAGC(-5/-2)
BclI          513  TGATCA               T^GATCA
BcnI          822  CCGGG                CC^[GC]GG
BglII         162  AGATCT               A^GATCT
BmyI          951  GGGCTC               G[AGT]GC[ACT]^C
BsaI         1128  GAGACC               GGTCTC(1/5)
BsmFI         966  GTCCC                GGGAC(10/14)
BsmI          491  GCATTC               GAATGC(1/-1)
BsoBI         968  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      951  GGGCTC               G[AGT]GC[ACT]^C
BsrDI        1097  CATTGC               GCAATG(2/0)
BsrFI         914  GCCGGC               [GA]^CCGG[CT]
BssSI         997  CTCGTG               CACGAG(-5/-1)
BstF5I        884  GGATG                GGATG(2/0)
BstXI        1132  CCATCTTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         162  AGATCT               [GA]^GATC[CT]
Cfr10I        914  GCCGGC               [GA]^CCGG[CT]
DraII         964  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          677  CCATGG               C^C[GA][CT]GG
Eam1105I      958  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      944  AGCGCT               AGC^GCT
EcoO109I      964  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FbaI          513  TGATCA               T^GATCA
FokI          884  GGATG                GGATG(9/13)
HincII        671  GTCAAC               GT[CT]^[GA]AC
HindII        671  GTCAAC               GT[CT]^[GA]AC
MflI          162  AGATCT               [GA]^GATC[CT]
MseI          206  TTAA                 T^TAA
MslI         1133  CATCTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        807  CAGCGG               C[AC]G^C[GT]G
NaeI          914  GCCGGC               GCC^GGC
NciI          822  CCGGG                CC^[GC]GG
NcoI          677  CCATGG               C^CATGG
NgoMIV        914  GCCGGC               G^CCGGC
NspI         1022  ACATGC               [GA]CATG^[CT]
PflMI        1178  CCATCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         964  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1170  AACGTT               AA^CGTT
RsaI          712  GTAC                 GT^AC
SfaNI         885  GATGC                GCATC(5/9)
SmlI          894  CTTGAG               C^T[CT][GA]AG
Tru9I         206  TTAA                 T^TAA
Van91I       1178  CCATCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          668  CCAGTCAACCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         162  AGATCT               [GA]^GATC[CT]
XmnI          700  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI          809  GCGG                 CCGC(-3/-1)
              836  GCGG                
AluI          216  AGCT                 AG^CT
              619  AGCT                
AlwNI        1039  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1048  CAGAAACTG           
BbvI          974  GCAGC                GCAGC(8/12)
               90  GCTGC               
BseRI        1109  CTCCTC               GAGGAG(10/8)
              785  GAGGAG              
BspMI         744  GCAGGT               ACCTGC(4/8)
              918  GCAGGT              
BstNI         718  CCTGG                CC^[AT]GG
             1159  CCTGG               
Cac8I         914  GCCGGC               GC[ACGT]^[ACGT]GC
             1074  GCAGGC              
CfoI          814  GCGC                 GCG^C
              945  GCGC                
EcoRI         536  GAATTC               G^AATTC
              850  GAATTC              
EcoRII        718  CCTGG                ^CC[AT]GG
             1159  CCTGG               
EcoT14I       677  CCATGG               C^C[AT][AT]GG
              908  CCAAGG              
Fnu4HI         90  GCTGC                GC^[ACGT]GC
              974  GCAGC               
HaeII         813  AGCGCC               [GA]GCGC^[CT]
              944  AGCGCT              
HapII         822  CCGG                 C^CGG
              915  CCGG                
HhaI          814  GCGC                 GCG^C
              945  GCGC                
HinP1I        814  GCGC                 G^CGC
              945  GCGC                
HpaII         822  CCGG                 C^CGG
              915  CCGG                
ItaI           90  GCTGC                GC^[ACGT]GC
              974  GCAGC               
MaeII         625  ACGT                 A^CGT
             1171  ACGT                
MspI          822  CCGG                 C^CGG
              915  CCGG                
MvaI          718  CCTGG                CC^[AT]GG
             1159  CCTGG               
MwoI           16  GCCATGATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1069  GCTTTGCAGGC         
NdeI           26  CATATG               CA^TATG
             1182  CATATG              
PleI          241  GAGTC                GAGTC(4/5)
              607  GAGTC               
PstI         1012  CTGCAG               CTGCA^G
             1045  CTGCAG              
SfcI         1012  CTGCAG               C^T[GA][CT]AG
             1045  CTGCAG              
StyI          677  CCATGG               C^C[AT][AT]GG
              908  CCAAGG              
TaqI          122  TCGA                 T^CGA
             1148  TCGA                
TfiI          556  GATTC                G^A[AT]TC
              580  GATTC               
TseI           90  GCTGC                G^C[AT]GC
              974  GCAGC               
Tsp45I        227  GTGAC                ^GT[GC]AC
              349  GTCAC               
TthHB8I       122  TCGA                 T^CGA
             1148  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        ApoI        AvaII       BsaJI       BsiYI       
BslI        BsmAI       BsrI        BssKI       Cfr13I      
DdeI        DpnII       EarI        HaeIII      HinfI       
HphI        Ksp632I     MaeIII      MboI        MboII       
MnlI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       Tsp509I     TspRI       
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