Restriction map for pF1KA1996
 (Linear) (Six-base) MAPSORT of: KIAA1996.seq  Check: 5971  from: 1  to: 1248



KIAA1996.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     735     909    1248 
   Size:        735     174     339

  Fragments arranged by size: 

                 735     339     174
 
AclI AA'CG_TT

Cuts at:      0    1171    1248 
   Size:       1171      77
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    1021    1248 
   Size:        455     566     227

  Fragments arranged by size: 

                 566     455     227
 
AfeI AGC'GCT

Cuts at:      0     946    1248 
   Size:        946     302
 
AhdI GACnn_n'nnGTC

Cuts at:      0     963    1248 
   Size:        963     285
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     767    1248 
   Size:        767     481
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1044    1053    1248 
   Size:       1044       9     195

  Fragments arranged by size: 

                1044     195       9
 
ApoI r'AATT_y

Cuts at:      0     333     536     684     850    1248 
   Size:        333     203     148     166     398

  Fragments arranged by size: 

                 398     333     203     166     148
 
AvaI C'yCGr_G

Cuts at:      0     968    1248 
   Size:        968     280
 
BanII G_rGCy'C

Cuts at:      0     955    1248 
   Size:        955     293
 
BbvCI CC'TCA_GC

Cuts at:      0    1112    1248 
   Size:       1112     136
 
BclI T'GATC_A

Cuts at:      0     513    1248 
   Size:        513     735
 
BglII A'GATC_T

Cuts at:      0     162    1248 
   Size:        162    1086
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     994    1248 
   Size:        994     254
 
Bpu10I CC'TnA_GC

Cuts at:      0    1112    1248 
   Size:       1112     136
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     915    1248 
   Size:        915     333
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1122    1248 
   Size:       1122     126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     171     201    1248 
   Size:        171      30    1047

  Fragments arranged by size: 

                1047     171      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     800    1100    1248 
   Size:        800     300     148

  Fragments arranged by size: 

                 800     300     148
 
BseYI C'CCAG_C

Cuts at:      0     947    1248 
   Size:        947     301
 
BsmI GAATG_Cn'

Cuts at:      0     491    1248 
   Size:        491     757
 
Bsp1286I G_dGCh'C

Cuts at:      0     955    1248 
   Size:        955     293
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     735     909    1248 
   Size:        735     174     339

  Fragments arranged by size: 

                 735     339     174
 
BsrDI GCAATG_nn'

Cuts at:      0    1096    1248 
   Size:       1096     152
 
BsrFI r'CCGG_y

Cuts at:      0     914    1248 
   Size:        914     334
 
BssSI C'ACGA_G

Cuts at:      0     997    1248 
   Size:        997     251
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1139    1248 
   Size:       1139     109
 
BstYI r'GATC_y

Cuts at:      0     162    1248 
   Size:        162    1086
 
BtgI C'CryG_G

Cuts at:      0     677    1248 
   Size:        677     571
 
BtsI GCAGTG_nn'

Cuts at:      0     231    1248 
   Size:        231    1017
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     117     152    1248 
   Size:        117      35    1096

  Fragments arranged by size: 

                1096     117      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     248     599     861     867    1248 
   Size:        248     351     262       6     381

  Fragments arranged by size: 

                 381     351     262     248       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     851    1248 
   Size:        851     397
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    1021    1248 
   Size:        455     566     227

  Fragments arranged by size: 

                 566     455     227
 
EcoO109I rG'GnC_Cy

Cuts at:      0     965    1248 
   Size:        965     283
 
EcoRI G'AATT_C

Cuts at:      0     536     850    1248 
   Size:        536     314     398

  Fragments arranged by size: 

                 536     398     314
 
HaeII r_GCGC'y

Cuts at:      0     817     948    1248 
   Size:        817     131     300

  Fragments arranged by size: 

                 817     300     131
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     838     870     949     981    1095    1127 
   Size:        142      32     664      32      79      32     114      32

Cuts at:   1127    1248 
   Size:        121

  Fragments arranged by size: 

                 664     142     121     114      79      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     673    1248 
   Size:        673     575
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     653     980    1248 
   Size:        653     327     268

  Fragments arranged by size: 

                 653     327     268
 
MslI CAynn'nnrTG

Cuts at:      0    1137    1248 
   Size:       1137     111
 
MspA1I CmG'CkG

Cuts at:      0     809    1248 
   Size:        809     439
 
NaeI GCC'GGC

Cuts at:      0     916    1248 
   Size:        916     332
 
NcoI C'CATG_G

Cuts at:      0     677    1248 
   Size:        677     571
 
NdeI CA'TA_TG

Cuts at:      0      27    1183    1248 
   Size:         27    1156      65

  Fragments arranged by size: 

                1156      65      27
 
NgoMIV G'CCGG_C

Cuts at:      0     914    1248 
   Size:        914     334
 
NspI r_CATG'y

Cuts at:      0    1026    1248 
   Size:       1026     222
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1184    1248 
   Size:       1184      64
 
PfoI T'CCnGG_A

Cuts at:      0     717    1248 
   Size:        717     531
 
PpuMI rG'GwC_Cy

Cuts at:      0     965    1248 
   Size:        965     283
 
PstI C_TGCA'G

Cuts at:      0    1016    1049    1248 
   Size:       1016      33     199

  Fragments arranged by size: 

                1016     199      33
 
SanDI GG'GwC_CC

Cuts at:      0     965    1248 
   Size:        965     283
 
SfcI C'TryA_G

Cuts at:      0    1012    1045    1248 
   Size:       1012      33     203

  Fragments arranged by size: 

                1012     203      33
 
SmlI C'TyrA_G

Cuts at:      0     894    1248 
   Size:        894     354
 
StyI C'CwwG_G

Cuts at:      0     677     908    1248 
   Size:        677     231     340

  Fragments arranged by size: 

                 677     340     231
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     646    1248 
   Size:        646     602
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     171     203    1248 
   Size:        171      32    1045

  Fragments arranged by size: 

                1045     171      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     675    1248 
   Size:        675     573
 
XmnI GAAnn'nnTTC

Cuts at:      0     704    1248 
   Size:        704     544
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI     AhdI     AlfI    AlwNI     ApoI
     AvaI    BanII    BbvCI     BclI    BglII     BplI   Bpu10I    BpuEI
     BsaI    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BspMI    BsrDI
    BsrFI    BssSI    BstXI    BstYI     BtgI     BtsI    CspCI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I   HincII     MmeI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI    PflMI
     PfoI    PpuMI     PstI    SanDI     SfcI     SmlI     StyI    TaqII
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BpmI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NarI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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