Restriction map for pF1KE1082
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoRI       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RcaI        RsrII       SacI        SalI        
SapI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AclI           10  AACGTT               AA^CGTT
AcsI          154  AAATTC               [GA]^AATT[CT]
AcyI          129  GGCGCC               G[GA]^CG[CT]C
AfaI          528  GTAC                 GT^AC
ApoI          154  AAATTC               [GA]^AATT[CT]
Asp700I       150  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         715  GTGCTC               G[AT]GC[AT]^C
AvaI          703  CCCGAG               C^[CT]CG[GA]G
BbeI          129  GGCGCC               GGCGC^C
BbsI          580  GTCTTC               GAAGAC(2/6)
BfaI          188  CTAG                 C^TAG
BpuAI         580  GTCTTC               GAAGAC(2/6)
BsaHI         129  GGCGCC               G[GA]^CG[CT]C
BsaI          364  GAGACC               GGTCTC(1/5)
BsgI           55  GTGCAG               GTGCAG(16/14)
BsiHKAI       715  GTGCTC               G[AT]GC[AT]^C
BsmFI         183  GTCCC                GGGAC(10/14)
BsoBI         703  CCCGAG               C^[CT]CG[GA]G
BspDI         784  ATCGAT               AT^CGAT
BspMI         530  ACCTGC               ACCTGC(4/8)
BsrI          882  ACTGG                ACTGG(1/-1)
BssHII        463  GCGCGC               G^CGCGC
BstEII        303  GGTGACC              G^GT[ACGT]ACC
BstPI         303  GGTGACC              G^GT[ACGT]ACC
ClaI          784  ATCGAT               AT^CGAT
EarI         1246  GAAGAG               CTCTTC(1/4)
EcoO65I       303  GGTGACC              G^GT[ACGT]ACC
EcoRV         142  GATATC               GAT^ATC
EcoT14I      1172  CCAAGG               C^C[AT][AT]GG
HgaI          506  GCGTC                GACGC(5/10)
Hin1I         129  GGCGCC               G[GA]^CG[CT]C
KasI          129  GGCGCC               G^GCGCC
Ksp632I      1246  GAAGAG               CTCTTC(1/4)
KspI          495  CCGCGG               CCGC^GG
MaeI          188  CTAG                 C^TAG
MseI         1187  TTAA                 T^TAA
NarI          129  GGCGCC               GG^CGCC
PpuMI        1036  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I       10  AACGTT               AA^CGTT
PvuII         292  CAGCTG               CAG^CTG
RsaI          528  GTAC                 GT^AC
SacII         495  CCGCGG               CCGC^GG
SbfI          531  CCTGCAGG             CCTGCA^GG
SfaNI         563  GCATC                GCATC(5/9)
SfoI          129  GGCGCC               GGC^GCC
SmlI          889  CTCAAG               C^T[CT][GA]AG
Sse8387I      531  CCTGCAGG             CCTGCA^GG
StyI         1172  CCAAGG               C^C[AT][AT]GG
TfiI          661  GATTC                G^A[AT]TC
Tru9I        1187  TTAA                 T^TAA
Tsp509I       155  AATT                 ^AATT
XmnI          150  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         464  CGCG                 CG^CG
              496  CGCG                
AspI          678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              982  GACAGTGTC           
AvaII         885  GGTCC                G^G[AT]CC
             1037  GGTCC               
BanI          129  GGCGCC               G^G[CT][GA]CC
              896  GGTGCC              
BanII         202  GGGCTC               G[GA]GC[CT]^C
              706  GAGCCC              
BsiEI         284  CGGCCG               CG[GA][CT]^CG
              416  CGGCCG              
BsmBI         432  CGTCTC               CGTCTC(1/5)
              903  CGTCTC              
BsrBI         195  CCGCTC               CCGCTC(-3/-3)
              405  GAGCGG              
BstF5I        387  CATCC                GGATG(2/0)
             1206  CATCC               
BstUI         464  CGCG                 CG^CG
              496  CGCG                
BstYI         537  GGATCT               [GA]^GATC[CT]
             1139  AGATCC              
DraII        1036  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1166  AGGCCCC             
DsaI          281  CCACGG               C^C[GA][CT]GG
              495  CCGCGG              
EagI          284  CGGCCG               C^GGCCG
              416  CGGCCG              
EclXI         284  CGGCCG               C^GGCCG
              416  CGGCCG              
Eco52I        284  CGGCCG               C^GGCCG
              416  CGGCCG              
EcoO109I     1036  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1166  AGGCCCC             
FokI          387  CATCC                GGATG(9/13)
             1206  CATCC               
MflI          537  GGATCT               [GA]^GATC[CT]
             1139  AGATCC              
MvnI          464  CGCG                 CG^CG
              496  CGCG                
NlaIII        327  CATG                 CATG^
              721  CATG                
PflFI         678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              982  GACAGTGTC           
PstI          118  CTGCAG               CTGCA^G
              532  CTGCAG              
SfcI          118  CTGCAG               C^T[GA][CT]AG
              532  CTGCAG              
TspRI         881  CACTG                CA[GC]TG[ACGT][ACGT]^
              984  CAGTG               
Tth111I       678  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              982  GACAGTGTC           
XhoII         537  GGATCT               [GA]^GATC[CT]
             1139  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
TaqI        TseI        Tsp45I      TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com