Restriction map for pF1KB7932
 (Linear) (Six-base) MAPSORT of: KIBB7932.seq  Check: 9604  from: 1  to: 639



KIBB7932.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0     466     639 
   Size:        466     173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     339     639 
   Size:         95     244     300

  Fragments arranged by size: 

                 300     244      95
 
BanI G'GyrC_C

Cuts at:      0     100     145     639 
   Size:        100      45     494

  Fragments arranged by size: 

                 494     100      45
 
BanII G_rGCy'C

Cuts at:      0     212     639 
   Size:        212     427
 
BbeI G_GCGC'C

Cuts at:      0     104     149     639 
   Size:        104      45     490

  Fragments arranged by size: 

                 490     104      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     572     639 
   Size:         86     486      67

  Fragments arranged by size: 

                 486      86      67
 
BglI GCCn_nnn'nGGC

Cuts at:      0     261     639 
   Size:        261     378
 
BglII A'GATC_T

Cuts at:      0     558     639 
   Size:        558      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30     151     639 
   Size:         30     121     488

  Fragments arranged by size: 

                 488     121      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     613     639 
   Size:        472     141      26

  Fragments arranged by size: 

                 472     141      26
 
BsaHI Gr'CG_yC

Cuts at:      0      56     101     146     639 
   Size:         56      45      45     493

  Fragments arranged by size: 

                 493      56      45      45
 
BsaWI w'CCGG_w

Cuts at:      0     497     639 
   Size:        497     142
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     106     121     166     169     639 
   Size:         61      45      15      45       3     470

  Fragments arranged by size: 

                 470      61      45      45      15       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     639 
   Size:        601      38
 
BsiEI CG_ry'CG

Cuts at:      0     175     639 
   Size:        175     464
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     639 
   Size:        212     427
 
BspEI T'CCGG_A

Cuts at:      0     497     639 
   Size:        497     142
 
BsrBI CCG'CTC

Cuts at:      0     597     639 
   Size:        597      42
 
BsrDI GCAATG_nn'

Cuts at:      0     614     639 
   Size:        614      25
 
BstEII G'GTnAC_C

Cuts at:      0     340     639 
   Size:        340     299
 
BstYI r'GATC_y

Cuts at:      0     558     639 
   Size:        558      81
 
BstZ17I GTA'TAC

Cuts at:      0     467     639 
   Size:        467     172
 
BtgI C'CryG_G

Cuts at:      0     175     639 
   Size:        175     464
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     495     639 
   Size:        495     144
 
BtsI GCAGTG_nn'

Cuts at:      0     454     639 
   Size:        454     185
 
EaeI y'GGCC_r

Cuts at:      0     172     639 
   Size:        172     467
 
EagI C'GGCC_G

Cuts at:      0     172     639 
   Size:        172     467
 
EarI CTCTTCn'nnn_

Cuts at:      0      56     116     358     639 
   Size:         56      60     242     281

  Fragments arranged by size: 

                 281     242      60      56
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28     639 
   Size:         28     611
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     339     472     613     639 
   Size:         95     244     133     141      26

  Fragments arranged by size: 

                 244     141     133      95      26
 
HaeII r_GCGC'y

Cuts at:      0     104     149     639 
   Size:        104      45     490

  Fragments arranged by size: 

                 490     104      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     521     553     639 
   Size:        521      32      86

  Fragments arranged by size: 

                 521      86      32
 
HincII GTy'rAC

Cuts at:      0     390     426     639 
   Size:        390      36     213

  Fragments arranged by size: 

                 390     213      36
 
HpaI GTT'AAC

Cuts at:      0     390     639 
   Size:        390     249
 
KasI G'GCGC_C

Cuts at:      0     100     145     639 
   Size:        100      45     494

  Fragments arranged by size: 

                 494     100      45
 
MslI CAynn'nnrTG

Cuts at:      0     575     639 
   Size:        575      64
 
MspA1I CmG'CkG

Cuts at:      0     177     639 
   Size:        177     462
 
NarI GG'CG_CC

Cuts at:      0     101     146     639 
   Size:        101      45     493

  Fragments arranged by size: 

                 493     101      45
 
NotI GC'GGCC_GC

Cuts at:      0     172     639 
   Size:        172     467
 
SacII CC_GC'GG

Cuts at:      0     178     639 
   Size:        178     461
 
SapI GCTCTTCn'nnn_

Cuts at:      0     358     639 
   Size:        358     281
 
SfoI GGC'GCC

Cuts at:      0     102     147     639 
   Size:        102      45     492

  Fragments arranged by size: 

                 492     102      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     587     639 
   Size:        587      52
 
Tth111I GACn'n_nGTC

Cuts at:      0     538     639 
   Size:        538     101
 

 Enzymes that do cut:

     AccI     AcuI     BanI    BanII     BbeI     BbsI     BglI    BglII
     BmrI     BpmI    BsaHI    BsaWI    BseRI     BsgI    BsiEI Bsp1286I
    BspEI    BsrBI    BsrDI   BstEII    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI    HaeII    Hin4I
   HincII     HpaI     KasI     MslI   MspA1I     NarI     NotI    SacII
     SapI     SfoI    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseYI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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