Restriction map for pF1KB0471
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# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AspI        AvaI        AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BcgI        
BciVI       BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrFI       BsrGI       BssHII      BstBI       BstEII      
BstPI       BstUI       CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          207  GTATAC               GT^[AC][GT]AC
AclI          351  AACGTT               AA^CGTT
AfaI          184  GTAC                 GT^AC
AflII         813  CTTAAG               C^TTAAG
AflIII        392  ACATGT               A^C[GA][CT]GT
AlwNI         716  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1111  CCTGAGG              CC^T[ACGT]AGG
ApaI         1177  GGGCCC               GGGCC^C
AseI          442  ATTAAT               AT^TAAT
AsnI          442  ATTAAT               AT^TAAT
Asp700I        15  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1380  GGATCC               G^GATCC
BbvCI        1153  CCTCAGC              CCTCAGC(-5/-2)
BbvI          982  GCAGC                GCAGC(8/12)
BclI         1488  TGATCA               T^GATCA
BcnI         1083  CCCGG                CC^[GC]GG
BfaI          328  CTAG                 C^TAG
BfrI          813  CTTAAG               C^TTAAG
BpmI         1204  CTCCAG               CTGGAG(16/14)
BsaWI         143  TCCGGT               [AT]^CCGG[AT]
BsmBI        1349  CGTCTC               CGTCTC(1/5)
BsmFI        1426  GGGAC                GGGAC(10/14)
BsmI           15  GAATGC               GAATGC(1/-1)
BspHI         215  TCATGA               T^CATGA
BspLU11I      392  ACATGT               A^CATGT
BsrI         1071  CCAGT                ACTGG(1/-1)
Bst1107I      207  GTATAC               GTA^TAC
BstAPI        715  GCAGAATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1527  GGATG                GGATG(2/0)
BstXI         893  CCATGGATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1380  GGATCC               [GA]^GATC[CT]
BstZ17I       207  GTATAC               GTA^TAC
Bsu36I       1111  CCTGAGG              CC^T[ACGT]AGG
CfoI          985  GCGC                 GCG^C
Eco81I       1111  CCTGAGG              CC^T[ACGT]AGG
EcoRV         126  GATATC               GAT^ATC
EcoT22I      1015  ATGCAT               ATGCA^T
FbaI         1488  TGATCA               T^GATCA
Fnu4HI        982  GCAGC                GC^[ACGT]GC
FokI         1527  GGATG                GGATG(9/13)
HaeII         984  AGCGCC               [GA]GCGC^[CT]
HhaI          985  GCGC                 GCG^C
HinP1I        985  GCGC                 G^CGC
ItaI          982  GCAGC                GC^[ACGT]GC
MaeI          328  CTAG                 C^TAG
MaeII         352  ACGT                 A^CGT
MflI         1380  GGATCC               [GA]^GATC[CT]
NciI         1083  CCCGG                CC^[GC]GG
NcoI          893  CCATGG               C^CATGG
NdeI           83  CATATG               CA^TATG
NsiI         1015  ATGCAT               ATGCA^T
NspI          392  ACATGT               [GA]CATG^[CT]
PflMI        1404  CCATTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1208  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI         442  ATTAAT               AT^TAAT
Psp1406I      351  AACGTT               AA^CGTT
RcaI          215  TCATGA               T^CATGA
RsaI          184  GTAC                 GT^AC
SacI         1126  GAGCTC               GAGCT^C
TseI          982  GCAGC                G^C[AT]GC
Van91I       1404  CCATTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          893  CCATGGATATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1380  GGATCC               [GA]^GATC[CT]
XmnI           15  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AhdI         1022  GACTGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1081  GACCCGGAGTC         
AluI         1127  AGCT                 AG^CT
             1234  AGCT                
AlwI         1381  GATCC                GGATC(4/5)
             1380  GGATC               
AspEI        1022  GACTGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1081  GACCCGGAGTC         
AspHI         637  GAGCAC               G[AT]GC[AT]^C
             1126  GAGCTC              
BanII        1126  GAGCTC               G[GA]GC[CT]^C
             1177  GGGCCC              
BsiHKAI       637  GAGCAC               G[AT]GC[AT]^C
             1126  GAGCTC              
BsmAI         666  GTCTC                GTCTC(1/5)
             1350  GTCTC               
BspMI         875  ACCTGC               ACCTGC(4/8)
             1106  ACCTGC              
BsrDI         131  CATTGC               GCAATG(2/0)
              200  CATTGC              
BssSI         307  CACGAG               CACGAG(-5/-1)
               67  CTCGTG              
DsaI          893  CCATGG               C^C[GA][CT]GG
             1238  CCACGG              
Eam1105I     1022  GACTGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1081  GACCCGGAGTC         
EcoNI        1107  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1181  CCTGTGAGAGG         
EcoT14I       893  CCATGG               C^C[AT][AT]GG
             1521  CCTTGG              
FauI          341  GCGGG                CCCGC(4/6)
             1418  GCGGG               
HapII         144  CCGG                 C^CGG
             1084  CCGG                
HincII       1369  GTTGAC               GT[CT]^[GA]AC
             1516  GTTGAC              
HindII       1369  GTTGAC               GT[CT]^[GA]AC
             1516  GTTGAC              
HpaII         144  CCGG                 C^CGG
             1084  CCGG                
MspA1I        339  CAGCGG               C[AC]G^C[GT]G
             1416  CAGCGG              
MspI          144  CCGG                 C^CGG
             1084  CCGG                
MwoI          715  GCAGAATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1101  GCCTGACCTGC         
SfcI         1061  CTACAG               C^T[GA][CT]AG
             1230  CTACAG              
SmlI          813  CTTAAG               C^T[CT][GA]AG
             1170  CTTGAG              
StyI          893  CCATGG               C^C[AT][AT]GG
             1521  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AcsI        ApoI        AvaII       BalI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      HaeIII      HinfI       HphI        
Ksp632I     MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MvaI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       StuI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       
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