Restriction map for pF1KB0471
 (Linear) (Six-base) MAPSORT of: KIBB0471.seq  Check: 4623  from: 1  to: 1545



KIBB0471.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AccI GT'mk_AC

Cuts at:      0     208    1545 
   Size:        208    1337
 
AclI AA'CG_TT

Cuts at:      0     352    1545 
   Size:        352    1193
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12    1439    1545 
   Size:         12    1427     106

  Fragments arranged by size: 

                1427     106      12
 
AflII C'TTAA_G

Cuts at:      0     813    1545 
   Size:        813     732
 
AflIII A'CryG_T

Cuts at:      0     392    1545 
   Size:        392    1153
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1027    1086    1545 
   Size:       1027      59     459

  Fragments arranged by size: 

                1027     459      59
 
AleI CACnn'nnGTG

Cuts at:      0     781    1545 
   Size:        781     764
 
AlwNI CAG_nnn'CTG

Cuts at:      0     721    1545 
   Size:        721     824
 
ApaI G_GGCC'C

Cuts at:      0    1181    1545 
   Size:       1181     364
 
ApoI r'AATT_y

Cuts at:      0     138     457     573     840    1545 
   Size:        138     319     116     267     705

  Fragments arranged by size: 

                 705     319     267     138     116
 
AseI AT'TA_AT

Cuts at:      0     443    1545 
   Size:        443    1102
 
BamHI G'GATC_C

Cuts at:      0    1380    1545 
   Size:       1380     165
 
BanII G_rGCy'C

Cuts at:      0    1130    1181    1545 
   Size:       1130      51     364

  Fragments arranged by size: 

                1130     364      51
 
BbvCI CC'TCA_GC

Cuts at:      0    1154    1545 
   Size:       1154     391
 
BclI T'GATC_A

Cuts at:      0    1488    1545 
   Size:       1488      57
 
BfrBI ATG'CAT

Cuts at:      0    1017    1545 
   Size:       1017     528
 
Bme1580I G_kGCm'C

Cuts at:      0    1181    1545 
   Size:       1181     364
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1065    1545 
   Size:       1065     480
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    1545 
   Size:       1189     356
 
Bpu10I CC'TnA_GC

Cuts at:      0    1154    1545 
   Size:       1154     391
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1191    1545 
   Size:       1191     354
 
BsaWI w'CCGG_w

Cuts at:      0     143    1545 
   Size:        143    1402
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1182    1212    1219    1249    1545 
   Size:       1182      30       7      30     296

  Fragments arranged by size: 

                1182     296      30      30       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1108    1111    1203    1545 
   Size:       1108       3      92     342

  Fragments arranged by size: 

                1108     342      92       3
 
BseYI C'CCAG_C

Cuts at:      0    1444    1545 
   Size:       1444     101
 
BsiHKAI G_wGCw'C

Cuts at:      0     641    1130    1545 
   Size:        641     489     415

  Fragments arranged by size: 

                 641     489     415
 
BsmI GAATG_Cn'

Cuts at:      0      21    1545 
   Size:         21    1524
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1355    1545 
   Size:       1355     190
 
Bsp1286I G_dGCh'C

Cuts at:      0     641    1130    1181    1545 
   Size:        641     489      51     364

  Fragments arranged by size: 

                 641     489     364      51
 
BspHI T'CATG_A

Cuts at:      0     215    1545 
   Size:        215    1330
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     884    1115    1545 
   Size:        884     231     430

  Fragments arranged by size: 

                 884     430     231
 
BsrDI GCAATG_nn'

Cuts at:      0     130     199    1545 
   Size:        130      69    1346

  Fragments arranged by size: 

                1346     130      69
 
BssSI C'ACGA_G

Cuts at:      0      67     307    1545 
   Size:         67     240    1238

  Fragments arranged by size: 

                1238     240      67
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     721    1545 
   Size:        721     824
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     900    1545 
   Size:        900     645
 
BstYI r'GATC_y

Cuts at:      0    1380    1545 
   Size:       1380     165
 
BstZ17I GTA'TAC

Cuts at:      0     209    1545 
   Size:        209    1336
 
Bsu36I CC'TnA_GG

Cuts at:      0    1112    1545 
   Size:       1112     433
 
BtgI C'CryG_G

Cuts at:      0     893    1238    1545 
   Size:        893     345     307

  Fragments arranged by size: 

                 893     345     307
 
EaeI y'GGCC_r

Cuts at:      0     383     696     862    1412    1545 
   Size:        383     313     166     550     133

  Fragments arranged by size: 

                 550     383     313     166     133
 
EarI CTCTTCn'nnn_

Cuts at:      0    1217    1355    1458    1545 
   Size:       1217     138     103      87

  Fragments arranged by size: 

                1217     138     103      87
 
EcoICRI GAG'CTC

Cuts at:      0    1128    1545 
   Size:       1128     417
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12    1189    1439    1545 
   Size:         12    1177     250     106

  Fragments arranged by size: 

                1177     250     106      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1111    1185    1545 
   Size:       1111      74     360

  Fragments arranged by size: 

                1111     360      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1178    1209    1461    1545 
   Size:       1178      31     252      84

  Fragments arranged by size: 

                1178     252      84      31
 
EcoRV GAT'ATC

Cuts at:      0     128    1545 
   Size:        128    1417
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     687    1517    1545 
   Size:        687     830      28

  Fragments arranged by size: 

                 830     687      28
 
HaeII r_GCGC'y

Cuts at:      0     988    1545 
   Size:        988     557
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1013    1045    1072    1104    1182    1214    1219    1251 
   Size:       1013      32      27      32      78      32       5      32

Cuts at:   1251    1267    1299    1468    1500    1545 
   Size:         16      32     169      32      45

  Fragments arranged by size: 

                1013     169      78      45      32      32      32      32
                  32      32      27      16       5
 
HincII GTy'rAC

Cuts at:      0    1371    1518    1545 
   Size:       1371     147      27

  Fragments arranged by size: 

                1371     147      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     866    1142    1545 
   Size:        866     276     403

  Fragments arranged by size: 

                 866     403     276
 
MscI TGG'CCA

Cuts at:      0     385     698     864    1414    1545 
   Size:        385     313     166     550     131

  Fragments arranged by size: 

                 550     385     313     166     131
 
MslI CAynn'nnrTG

Cuts at:      0     391     443     445     644     781     792     898    1545 
   Size:        391      52       2     199     137      11     106     647

  Fragments arranged by size: 

                 647     391     199     137     106      52      11       2
            
 
MspA1I CmG'CkG

Cuts at:      0     341    1418    1545 
   Size:        341    1077     127

  Fragments arranged by size: 

                1077     341     127
 
NcoI C'CATG_G

Cuts at:      0     893    1545 
   Size:        893     652
 
NdeI CA'TA_TG

Cuts at:      0      84    1545 
   Size:         84    1461
 
NsiI A_TGCA'T

Cuts at:      0    1019    1545 
   Size:       1019     526
 
NspI r_CATG'y

Cuts at:      0     396    1545 
   Size:        396    1149
 
PciI A'CATG_T

Cuts at:      0     392    1545 
   Size:        392    1153
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1410    1545 
   Size:       1410     135
 
PfoI T'CCnGG_A

Cuts at:      0    1205    1545 
   Size:       1205     340
 
PpuMI rG'GwC_Cy

Cuts at:      0    1209    1545 
   Size:       1209     336
 
PsiI TTA'TAA

Cuts at:      0     947    1545 
   Size:        947     598
 
PspOMI G'GGCC_C

Cuts at:      0    1177    1545 
   Size:       1177     368
 
SacI G_AGCT'C

Cuts at:      0    1130    1545 
   Size:       1130     415
 
SfcI C'TryA_G

Cuts at:      0    1061    1230    1545 
   Size:       1061     169     315

  Fragments arranged by size: 

                1061     315     169
 
SmlI C'TyrA_G

Cuts at:      0     813    1170    1545 
   Size:        813     357     375

  Fragments arranged by size: 

                 813     375     357
 
StuI AGG'CCT

Cuts at:      0     313    1035    1048    1545 
   Size:        313     722      13     497

  Fragments arranged by size: 

                 722     497     313      13
 
StyI C'CwwG_G

Cuts at:      0     893    1521    1545 
   Size:        893     628      24

  Fragments arranged by size: 

                 893     628      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     672     704    1545 
   Size:        672      32     841

  Fragments arranged by size: 

                 841     672      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     900    1545 
   Size:        900     645
 
XmnI GAAnn'nnTTC

Cuts at:      0      19    1545 
   Size:         19    1526
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AhdI     AleI    AlwNI
     ApaI     ApoI     AseI    BamHI    BanII    BbvCI     BclI    BfrBI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NsiI     NspI     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI
     SacI     SfcI     SmlI     StuI     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AlfI     AloI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
     BcgI    BciVI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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