Restriction map for pF1KB6688
 (Linear) (Six-base) MAPSORT of: KIBB6688.seq  Check: 1274  from: 1  to: 1374



KIBB6688.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AatII G_ACGT'C

Cuts at:      0     885    1374 
   Size:        885     489
 
AccI GT'mk_AC

Cuts at:      0    1102    1374 
   Size:       1102     272
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1374 
   Size:        811     563
 
AgeI A'CCGG_T

Cuts at:      0    1263    1374 
   Size:       1263     111
 
AhdI GACnn_n'nnGTC

Cuts at:      0     313    1374 
   Size:        313    1061
 
ApaLI G'TGCA_C

Cuts at:      0      37    1374 
   Size:         37    1337
 
ApoI r'AATT_y

Cuts at:      0     139     636    1118    1317    1374 
   Size:        139     497     482     199      57

  Fragments arranged by size: 

                 497     482     199     139      57
 
AvrII C'CTAG_G

Cuts at:      0     886    1374 
   Size:        886     488
 
BanI G'GyrC_C

Cuts at:      0     892     971    1374 
   Size:        892      79     403

  Fragments arranged by size: 

                 892     403      79
 
BanII G_rGCy'C

Cuts at:      0      85    1295    1374 
   Size:         85    1210      79

  Fragments arranged by size: 

                1210      85      79
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     603     637    1374 
   Size:        603      34     737

  Fragments arranged by size: 

                 737     603      34
 
BclI T'GATC_A

Cuts at:      0     221     962    1374 
   Size:        221     741     412

  Fragments arranged by size: 

                 741     412     221
 
Bme1580I G_kGCm'C

Cuts at:      0      41     382     895     976    1374 
   Size:         41     341     513      81     398

  Fragments arranged by size: 

                 513     398     341      81      41
 
BmgBI CAC'GTC

Cuts at:      0     617    1374 
   Size:        617     757
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79    1374 
   Size:         79    1295
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1326    1374 
   Size:       1326      48
 
BsaAI yAC'GTr

Cuts at:      0     469     698    1374 
   Size:        469     229     676

  Fragments arranged by size: 

                 676     469     229
 
BsaHI Gr'CG_yC

Cuts at:      0     882    1374 
   Size:        882     492
 
BsaWI w'CCGG_w

Cuts at:      0    1263    1374 
   Size:       1263     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     730    1374 
   Size:        235     495     644

  Fragments arranged by size: 

                 644     495     235
 
BsiEI CG_ry'CG

Cuts at:      0     815    1374 
   Size:        815     559
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     626    1374 
   Size:         41     585     748

  Fragments arranged by size: 

                 748     585      41
 
BsmI GAATG_Cn'

Cuts at:      0      21      24    1132    1374 
   Size:         21       3    1108     242

  Fragments arranged by size: 

                1108     242      21       3
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     873    1374 
   Size:        873     501
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      85     382     626     895     976    1295    1374 
   Size:         41      44     297     244     269      81     319      79

  Fragments arranged by size: 

                 319     297     269     244      81      79      44      41
            
 
BspHI T'CATG_A

Cuts at:      0     194     515    1374 
   Size:        194     321     859

  Fragments arranged by size: 

                 859     321     194
 
BsrDI GCAATG_nn'

Cuts at:      0     287    1374 
   Size:        287    1087
 
BsrFI r'CCGG_y

Cuts at:      0    1263    1374 
   Size:       1263     111
 
BstBI TT'CG_AA

Cuts at:      0     928    1374 
   Size:        928     446
 
BstZ17I GTA'TAC

Cuts at:      0    1103    1374 
   Size:       1103     271
 
Bsu36I CC'TnA_GG

Cuts at:      0      10    1374 
   Size:         10    1364
 
BtsI GCAGTG_nn'

Cuts at:      0    1208    1374 
   Size:       1208     166
 
DraIII CAC_nnn'GTG

Cuts at:      0     617    1374 
   Size:        617     757
 
EaeI y'GGCC_r

Cuts at:      0     163     358     750     812    1374 
   Size:        163     195     392      62     562

  Fragments arranged by size: 

                 562     392     195     163      62
 
EagI C'GGCC_G

Cuts at:      0     812    1374 
   Size:        812     562
 
EarI CTCTTCn'nnn_

Cuts at:      0     815    1374 
   Size:        815     559
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     811    1326    1374 
   Size:        811     515      48

  Fragments arranged by size: 

                 811     515      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     653    1374 
   Size:        653     721
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     941    1374 
   Size:        941     433
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     299     331    1374 
   Size:        299      32    1043

  Fragments arranged by size: 

                1043     299      32
 
HincII GTy'rAC

Cuts at:      0    1239    1374 
   Size:       1239     135
 
HindIII A'AGCT_T

Cuts at:      0     731     923    1042    1374 
   Size:        731     192     119     332

  Fragments arranged by size: 

                 731     332     192     119
 
HpaI GTT'AAC

Cuts at:      0    1239    1374 
   Size:       1239     135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1163    1374 
   Size:       1163     211
 
MscI TGG'CCA

Cuts at:      0     165     360    1374 
   Size:        165     195    1014

  Fragments arranged by size: 

                1014     195     165
 
MslI CAynn'nnrTG

Cuts at:      0     217     568    1256    1374 
   Size:        217     351     688     118

  Fragments arranged by size: 

                 688     351     217     118
 
MspA1I CmG'CkG

Cuts at:      0     678    1374 
   Size:        678     696
 
PpuMI rG'GwC_Cy

Cuts at:      0     653    1374 
   Size:        653     721
 
PsiI TTA'TAA

Cuts at:      0    1160    1374 
   Size:       1160     214
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1093    1125    1374 
   Size:       1093      32     249

  Fragments arranged by size: 

                1093     249      32
 
SfcI C'TryA_G

Cuts at:      0      95     545    1374 
   Size:         95     450     829

  Fragments arranged by size: 

                 829     450      95
 
SnaBI TAC'GTA

Cuts at:      0     469     698    1374 
   Size:        469     229     676

  Fragments arranged by size: 

                 676     469     229
 
SspI AAT'ATT

Cuts at:      0     993    1374 
   Size:        993     381
 
StyI C'CwwG_G

Cuts at:      0     886     936    1374 
   Size:        886      50     438

  Fragments arranged by size: 

                 886     438      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1353    1374 
   Size:       1353      21
 
Tth111I GACn'n_nGTC

Cuts at:      0     776    1374 
   Size:        776     598
 
XmnI GAAnn'nnTTC

Cuts at:      0     924    1043    1374 
   Size:        924     119     331

  Fragments arranged by size: 

                 924     331     119
 
ZraI GAC'GTC

Cuts at:      0     883    1374 
   Size:        883     491
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AgeI     AhdI    ApaLI     ApoI    AvrII
     BanI    BanII     BcgI     BclI Bme1580I    BmgBI     BmrI     BpmI
    BsaAI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrDI    BsrFI    BstBI  BstZ17I   Bsu36I     BtsI
   DraIII     EaeI     EagI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I    PpuMI
     PsiI     PsrI     SfcI    SnaBI     SspI     StyI     TstI  Tth111I
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII
     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI
    BseRI    BseYI    BsiWI    BspEI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     ClaI
    CspCI     DraI     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     XbaI     XcmI
     XhoI     XmaI
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