Restriction map for pF1KB0437
 (Linear) (Six-base) MAPSORT of: KIBB0437.seq  Check: 3909  from: 1  to: 1443



KIBB0437.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

Acc65I G'GTAC_C

Cuts at:      0     613    1443 
   Size:        613     830
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     836     853     902     937    1138    1336    1443 
   Size:        836      17      49      35     201     198     107

  Fragments arranged by size: 

                 836     201     198     107      49      35      17
 
AflIII A'CryG_T

Cuts at:      0     821    1443 
   Size:        821     622
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     313     345    1073    1105    1443 
   Size:        313      32     728      32     338

  Fragments arranged by size: 

                 728     338     313      32      32
 
ApoI r'AATT_y

Cuts at:      0     455     829    1039    1443 
   Size:        455     374     210     404

  Fragments arranged by size: 

                 455     404     374     210
 
AvaI C'yCGr_G

Cuts at:      0     138    1443 
   Size:        138    1305
 
BamHI G'GATC_C

Cuts at:      0     359    1443 
   Size:        359    1084
 
BanI G'GyrC_C

Cuts at:      0     613    1375    1443 
   Size:        613     762      68

  Fragments arranged by size: 

                 762     613      68
 
BanII G_rGCy'C

Cuts at:      0     420     636     877    1050    1443 
   Size:        420     216     241     173     393

  Fragments arranged by size: 

                 420     393     241     216     173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     824    1443 
   Size:        824     619
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1020    1443 
   Size:       1020     423
 
BclI T'GATC_A

Cuts at:      0    1205    1443 
   Size:       1205     238
 
BfrBI ATG'CAT

Cuts at:      0    1013    1443 
   Size:       1013     430
 
BglII A'GATC_T

Cuts at:      0     837    1319    1443 
   Size:        837     482     124

  Fragments arranged by size: 

                 837     482     124
 
Bme1580I G_kGCm'C

Cuts at:      0    1378    1443 
   Size:       1378      65
 
BmgBI CAC'GTC

Cuts at:      0     822     902    1443 
   Size:        822      80     541

  Fragments arranged by size: 

                 822     541      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     170    1381    1443 
   Size:        170    1211      62

  Fragments arranged by size: 

                1211     170      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     434    1106    1382    1443 
   Size:        100     334     672     276      61

  Fragments arranged by size: 

                 672     334     276     100      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     989    1443 
   Size:        989     454
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      43      58      73    1443 
   Size:         28      15      15      15    1370

  Fragments arranged by size: 

                1370      28      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      78     103     943    1443 
   Size:         78      25     840     500

  Fragments arranged by size: 

                 840     500      78      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     459     704    1443 
   Size:        234     225     245     739

  Fragments arranged by size: 

                 739     245     234     225
 
BsiHKAI G_wGCw'C

Cuts at:      0     636    1050    1077    1443 
   Size:        636     414      27     366

  Fragments arranged by size: 

                 636     414     366      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     420     636     877    1050    1077    1378    1443 
   Size:        420     216     241     173      27     301      65

  Fragments arranged by size: 

                 420     301     241     216     173      65      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1138    1443 
   Size:       1138     305
 
BsrDI GCAATG_nn'

Cuts at:      0     672     939    1443 
   Size:        672     267     504

  Fragments arranged by size: 

                 672     504     267
 
BsrFI r'CCGG_y

Cuts at:      0    1348    1443 
   Size:       1348      95
 
BstYI r'GATC_y

Cuts at:      0     108     359     837    1319    1443 
   Size:        108     251     478     482     124

  Fragments arranged by size: 

                 482     478     251     124     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     758    1443 
   Size:        758     685
 
BtsI GCAGTG_nn'

Cuts at:      0     692    1359    1443 
   Size:        692     667      84

  Fragments arranged by size: 

                 692     667      84
 
DraIII CAC_nnn'GTG

Cuts at:      0     437     450    1443 
   Size:        437      13     993

  Fragments arranged by size: 

                 993     437      13
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     187    1443 
   Size:        187    1256
 
EaeI y'GGCC_r

Cuts at:      0     474    1343    1443 
   Size:        474     869     100

  Fragments arranged by size: 

                 869     474     100
 
EarI CTCTTCn'nnn_

Cuts at:      0      96     340     872    1443 
   Size:         96     244     532     571

  Fragments arranged by size: 

                 571     532     244      96
 
EcoICRI GAG'CTC

Cuts at:      0     634    1048    1443 
   Size:        634     414     395

  Fragments arranged by size: 

                 634     414     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     434     836     853     902     937    1106    1138 
   Size:        100     334     402      17      49      35     169      32

Cuts at:   1138    1336    1382    1443 
   Size:        198      46      61

  Fragments arranged by size: 

                 402     334     198     169     100      61      49      46
                  35      32      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135    1081    1443 
   Size:        135     946     362

  Fragments arranged by size: 

                 946     362     135
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     859    1443 
   Size:        859     584
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     370     402    1443 
   Size:         28      32     310      32    1041

  Fragments arranged by size: 

                1041     310      32      32      28
 
HindIII A'AGCT_T

Cuts at:      0     884    1443 
   Size:        884     559
 
KpnI G_GTAC'C

Cuts at:      0     617    1443 
   Size:        617     826
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1443 
   Size:        296    1147
 
MscI TGG'CCA

Cuts at:      0     476    1443 
   Size:        476     967
 
MslI CAynn'nnrTG

Cuts at:      0    1226    1443 
   Size:       1226     217
 
MspA1I CmG'CkG

Cuts at:      0      99     370    1392    1443 
   Size:         99     271    1022      51

  Fragments arranged by size: 

                1022     271      99      51
 
NaeI GCC'GGC

Cuts at:      0    1350    1443 
   Size:       1350      93
 
NdeI CA'TA_TG

Cuts at:      0    1015    1200    1443 
   Size:       1015     185     243

  Fragments arranged by size: 

                1015     243     185
 
NgoMIV G'CCGG_C

Cuts at:      0    1348    1443 
   Size:       1348      95
 
NsiI A_TGCA'T

Cuts at:      0    1015    1443 
   Size:       1015     428
 
NspI r_CATG'y

Cuts at:      0     303     513    1443 
   Size:        303     210     930

  Fragments arranged by size: 

                 930     303     210
 
PasI CC'CwG_GG

Cuts at:      0      64    1443 
   Size:         64    1379
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486    1443 
   Size:        454      32     957

  Fragments arranged by size: 

                 957     454      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     135    1081    1443 
   Size:        135     946     362

  Fragments arranged by size: 

                 946     362     135
 
PstI C_TGCA'G

Cuts at:      0     702    1443 
   Size:        702     741
 
PvuII CAG'CTG

Cuts at:      0      99     370    1443 
   Size:         99     271    1073

  Fragments arranged by size: 

                1073     271      99
 
SacI G_AGCT'C

Cuts at:      0     636    1050    1443 
   Size:        636     414     393

  Fragments arranged by size: 

                 636     414     393
 
SapI GCTCTTCn'nnn_

Cuts at:      0     872    1443 
   Size:        872     571
 
SfcI C'TryA_G

Cuts at:      0     283     698    1443 
   Size:        283     415     745

  Fragments arranged by size: 

                 745     415     283
 
StuI AGG'CCT

Cuts at:      0      10    1443 
   Size:         10    1433
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      43      75    1443 
   Size:         43      32    1368

  Fragments arranged by size: 

                1368      43      32
 
XbaI T'CTAG_A

Cuts at:      0     555    1443 
   Size:        555     888
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     159    1443 
   Size:        159    1284
 
XmnI GAAnn'nnTTC

Cuts at:      0     839     866    1443 
   Size:        839      27     577

  Fragments arranged by size: 

                 839     577      27
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AloI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BstYI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I  HindIII
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV
     NsiI     NspI     PasI     PpiI    PpuMI     PstI    PvuII     SacI
     SapI     SfcI     StuI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI     BglI     BlpI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     MfeI     MluI     NarI     NcoI     NheI     NotI     NruI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI  Tth111I     XhoI     XmaI     ZraI
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