Restriction map for pF1KB0417
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       FseI        FspI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspA1I      MunI        NcoI        NdeI        
NheI        NotI        NruI        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         100  CGCG                 CG^CG
AcyI           86  GGCGCC               G[GA]^CG[CT]C
AfaI          852  GTAC                 GT^AC
AlwNI        1138  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1346  GGGCCC               GGGCC^C
AspI         1105  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         607  GGATCC               G^GATCC
BanI           86  GGCGCC               G^G[CT][GA]CC
BbeI           86  GGCGCC               GGCGC^C
BclI          113  TGATCA               T^GATCA
BcnI         1344  CCGGG                CC^[GC]GG
BfaI          925  CTAG                 C^TAG
BsaHI          86  GGCGCC               G[GA]^CG[CT]C
BsmAI         486  GAGAC                GTCTC(1/5)
BsmBI         486  GAGACG               CGTCTC(1/5)
BsmFI         865  GGGAC                GGGAC(10/14)
BspMI         257  GCAGGT               ACCTGC(4/8)
BsrDI         717  CATTGC               GCAATG(2/0)
BstAPI        357  GCAACGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         769  TTCGAA               TT^CGAA
BstUI         100  CGCG                 CG^CG
DraII         865  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1295  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      865  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV        1225  GATATC               GAT^ATC
EcoT22I       549  ATGCAT               ATGCA^T
FbaI          113  TGATCA               T^GATCA
HaeII          86  GGCGCC               [GA]GCGC^[CT]
Hin1I          86  GGCGCC               G[GA]^CG[CT]C
HincII        171  GTTAAC               GT[CT]^[GA]AC
HindII        171  GTTAAC               GT[CT]^[GA]AC
HpaI          171  GTTAAC               GTT^AAC
KasI           86  GGCGCC               G^GCGCC
MaeI          925  CTAG                 C^TAG
MaeII         299  ACGT                 A^CGT
MvnI          100  CGCG                 CG^CG
NaeI           83  GCCGGC               GCC^GGC
NarI           86  GGCGCC               GG^CGCC
NciI         1344  CCGGG                CC^[GC]GG
NgoMIV         83  GCCGGC               G^CCGGC
NsiI          549  ATGCAT               ATGCA^T
NspV          769  TTCGAA               TT^CGAA
PflFI        1105  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         865  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          255  CTGCAG               CTGCA^G
RsaI          852  GTAC                 GT^AC
ScaI          851  AGTACT               AGT^ACT
SfoI           86  GGCGCC               GGC^GCC
SfuI          769  TTCGAA               TT^CGAA
SmlI         1019  CTCAAG               C^T[CT][GA]AG
StuI          945  AGGCCT               AGG^CCT
TaqI          770  TCGA                 T^CGA
Tsp45I        820  GTGAC                ^GT[GC]AC
Tth111I      1105  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       770  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcsI            6  AAATTT               [GA]^AATT[CT]
              766  AAATTC              
AocI          870  CCTGAGG              CC^T[ACGT]AGG
             1135  CCTCAGG             
ApoI            6  AAATTT               [GA]^AATT[CT]
              766  AAATTC              
Asp700I       186  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              762  GAAGAAATTC          
AspHI         331  GAGCTC               G[AT]GC[AT]^C
              671  GAGCTC              
AvaI          862  CTCGGG               C^[CT]CG[GA]G
              941  CCCGAG              
BalI           30  TGGCCA               TGG^CCA
              680  TGGCCA              
BbsI          427  GAAGAC               GAAGAC(2/6)
             1303  GAAGAC              
BpuAI         427  GAAGAC               GAAGAC(2/6)
             1303  GAAGAC              
BseRI         150  GAGGAG               GAGGAG(10/8)
              208  GAGGAG              
BsiHKAI       331  GAGCTC               G[AT]GC[AT]^C
              671  GAGCTC              
BsoBI         862  CTCGGG               C^[CT]CG[GA]G
              941  CCCGAG              
BsrFI          83  GCCGGC               [GA]^CCGG[CT]
              953  ACCGGC              
BstXI         839  CCATTGTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1073  CCATGAGTTTGG        
Bsu36I        870  CCTGAGG              CC^T[ACGT]AGG
             1135  CCTCAGG             
CfoI           87  GCGC                 GCG^C
              915  GCGC                
Cfr10I         83  GCCGGC               [GA]^CCGG[CT]
              953  ACCGGC              
DsaI          613  CCGTGG               C^C[GA][CT]GG
              984  CCACGG              
EaeI           30  TGGCCA               [CT]^GGCC[GA]
              680  TGGCCA              
Eco81I        870  CCTGAGG              CC^T[ACGT]AGG
             1135  CCTCAGG             
EcoNI          27  CCTTGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              204  CCTGGAGGAGG         
FauI          564  CCCGC                CCCGC(4/6)
             1323  GCGGG               
HgaI          101  GCGTC                GACGC(5/10)
              391  GCGTC               
HhaI           87  GCGC                 GCG^C
              915  GCGC                
HinP1I         87  GCGC                 G^CGC
              915  GCGC                
HindIII       476  AAGCTT               A^AGCTT
             1036  AAGCTT              
MluNI          30  TGGCCA               TGG^CCA
              680  TGGCCA              
MscI           30  TGGCCA               TGG^CCA
              680  TGGCCA              
MseI          172  TTAA                 T^TAA
             1387  TTAA                
NspI          362  GCATGC               [GA]CATG^[CT]
              551  GCATGC              
PleI          822  GACTC                GAGTC(4/5)
             1109  GAGTC               
SacI          331  GAGCTC               GAGCT^C
              671  GAGCTC              
SfcI          255  CTGCAG               C^T[GA][CT]AG
             1118  CTACAG              
SphI          362  GCATGC               GCATG^C
              551  GCATGC              
Tru9I         172  TTAA                 T^TAA
             1387  TTAA                
XmnI          186  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              762  GAAGAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        TseI        Tsp509I     TspRI       XhoII       
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