Restriction map for pF1KB0417
 (Linear) (Six-base) MAPSORT of: KIBB0417.seq  Check: 2128  from: 1  to: 1413



KIBB0417.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1298    1413 
   Size:        331     967     115

  Fragments arranged by size: 

                 967     331     115
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1143    1413 
   Size:       1143     270
 
ApaI G_GGCC'C

Cuts at:      0    1350    1413 
   Size:       1350      63
 
ApoI r'AATT_y

Cuts at:      0       6     766    1413 
   Size:          6     760     647

  Fragments arranged by size: 

                 760     647       6
 
AvaI C'yCGr_G

Cuts at:      0     862     941    1413 
   Size:        862      79     472

  Fragments arranged by size: 

                 862     472      79
 
BamHI G'GATC_C

Cuts at:      0     607    1413 
   Size:        607     806
 
BanI G'GyrC_C

Cuts at:      0      86    1413 
   Size:         86    1327
 
BanII G_rGCy'C

Cuts at:      0     335     675    1350    1413 
   Size:        335     340     675      63

  Fragments arranged by size: 

                 675     340     335      63
 
BbeI G_GCGC'C

Cuts at:      0      90    1413 
   Size:         90    1323
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     434    1310    1413 
   Size:        434     876     103

  Fragments arranged by size: 

                 876     434     103
 
BclI T'GATC_A

Cuts at:      0     113    1413 
   Size:        113    1300
 
BfrBI ATG'CAT

Cuts at:      0     551    1413 
   Size:        551     862
 
Bme1580I G_kGCm'C

Cuts at:      0     161     402    1350    1413 
   Size:        161     241     948      63

  Fragments arranged by size: 

                 948     241     161      63
 
BmgBI CAC'GTC

Cuts at:      0     300    1413 
   Size:        300    1113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1306    1413 
   Size:       1306     107
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     145     226     659     688    1241    1413 
   Size:        118      27      81     433      29     553     172

  Fragments arranged by size: 

                 553     433     172     118      81      29      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     625    1413 
   Size:        625     788
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1004    1413 
   Size:       1004     409
 
BsaHI Gr'CG_yC

Cuts at:      0      87    1413 
   Size:         87    1326
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     807     837     965     995    1413 
   Size:        807      30     128      30     418

  Fragments arranged by size: 

                 807     418     128      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     165     223    1413 
   Size:        165      58    1190

  Fragments arranged by size: 

                1190     165      58
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     675    1413 
   Size:        335     340     738

  Fragments arranged by size: 

                 738     340     335
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     480    1413 
   Size:        480     933
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     335     402     675    1350    1413 
   Size:        161     174      67     273     675      63

  Fragments arranged by size: 

                 675     273     174     161      67      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     248    1413 
   Size:        248    1165
 
BsrDI GCAATG_nn'

Cuts at:      0     716    1413 
   Size:        716     697
 
BsrFI r'CCGG_y

Cuts at:      0      83     953    1413 
   Size:         83     870     460

  Fragments arranged by size: 

                 870     460      83
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     363    1413 
   Size:        363    1050
 
BstBI TT'CG_AA

Cuts at:      0     770    1413 
   Size:        770     643
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     846    1080    1413 
   Size:        846     234     333

  Fragments arranged by size: 

                 846     333     234
 
BstYI r'GATC_y

Cuts at:      0     244     560     607    1256    1413 
   Size:        244     316      47     649     157

  Fragments arranged by size: 

                 649     316     244     157      47
 
Bsu36I CC'TnA_GG

Cuts at:      0     871    1136    1413 
   Size:        871     265     277

  Fragments arranged by size: 

                 871     277     265
 
BtgI C'CryG_G

Cuts at:      0     613     984    1413 
   Size:        613     371     429

  Fragments arranged by size: 

                 613     429     371
 
DraIII CAC_nnn'GTG

Cuts at:      0    1300    1413 
   Size:       1300     113
 
EaeI y'GGCC_r

Cuts at:      0      30     680    1413 
   Size:         30     650     733

  Fragments arranged by size: 

                 733     650      30
 
EarI CTCTTCn'nnn_

Cuts at:      0      50     295     450    1413 
   Size:         50     245     155     963

  Fragments arranged by size: 

                 963     245     155      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     815    1413 
   Size:        815     598
 
EcoICRI GAG'CTC

Cuts at:      0     333     673    1413 
   Size:        333     340     740

  Fragments arranged by size: 

                 740     340     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     145     226     331     659     688    1241    1298 
   Size:        118      27      81     105     328      29     553      57

Cuts at:   1298    1413 
   Size:        115

  Fragments arranged by size: 

                 553     328     118     115     105      81      57      29
                  27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      31     208    1413 
   Size:         31     177    1205

  Fragments arranged by size: 

                1205     177      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     866    1413 
   Size:        866     547
 
EcoRV GAT'ATC

Cuts at:      0    1227    1413 
   Size:       1227     186
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1054    1413 
   Size:       1054     359
 
HaeII r_GCGC'y

Cuts at:      0      90    1413 
   Size:         90    1323
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     105     137     236     268     482     514     813     845 
   Size:        105      32      99      32     214      32     299      32

Cuts at:    845    1159    1191    1413 
   Size:        314      32     222

  Fragments arranged by size: 

                 314     299     222     214     105      99      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     173    1413 
   Size:        173    1240
 
HindIII A'AGCT_T

Cuts at:      0     476    1036    1413 
   Size:        476     560     377

  Fragments arranged by size: 

                 560     476     377
 
HpaI GTT'AAC

Cuts at:      0     173    1413 
   Size:        173    1240
 
KasI G'GCGC_C

Cuts at:      0      86    1413 
   Size:         86    1327
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1413 
   Size:        863     550
 
MscI TGG'CCA

Cuts at:      0      32     682    1413 
   Size:         32     650     731

  Fragments arranged by size: 

                 731     650      32
 
MslI CAynn'nnrTG

Cuts at:      0     181     546     844    1413 
   Size:        181     365     298     569

  Fragments arranged by size: 

                 569     365     298     181
 
NaeI GCC'GGC

Cuts at:      0      85    1413 
   Size:         85    1328
 
NarI GG'CG_CC

Cuts at:      0      87    1413 
   Size:         87    1326
 
NgoMIV G'CCGG_C

Cuts at:      0      83    1413 
   Size:         83    1330
 
NsiI A_TGCA'T

Cuts at:      0     553    1413 
   Size:        553     860
 
NspI r_CATG'y

Cuts at:      0     366     555    1413 
   Size:        366     189     858

  Fragments arranged by size: 

                 858     366     189
 
PfoI T'CCnGG_A

Cuts at:      0     134    1413 
   Size:        134    1279
 
PpuMI rG'GwC_Cy

Cuts at:      0     866    1413 
   Size:        866     547
 
PsiI TTA'TAA

Cuts at:      0     653    1413 
   Size:        653     760
 
PspOMI G'GGCC_C

Cuts at:      0    1346    1413 
   Size:       1346      67
 
PstI C_TGCA'G

Cuts at:      0     259    1413 
   Size:        259    1154
 
SacI G_AGCT'C

Cuts at:      0     335     675    1413 
   Size:        335     340     738

  Fragments arranged by size: 

                 738     340     335
 
SanDI GG'GwC_CC

Cuts at:      0     866    1413 
   Size:        866     547
 
ScaI AGT'ACT

Cuts at:      0     853    1413 
   Size:        853     560
 
SfcI C'TryA_G

Cuts at:      0     255    1118    1413 
   Size:        255     863     295

  Fragments arranged by size: 

                 863     295     255
 
SfoI GGC'GCC

Cuts at:      0      88    1413 
   Size:         88    1325
 
SmlI C'TyrA_G

Cuts at:      0    1019    1413 
   Size:       1019     394
 
SphI G_CATG'C

Cuts at:      0     366     555    1413 
   Size:        366     189     858

  Fragments arranged by size: 

                 858     366     189
 
StuI AGG'CCT

Cuts at:      0     947    1413 
   Size:        947     466
 
StyI C'CwwG_G

Cuts at:      0      27     141     384    1413 
   Size:         27     114     243    1029

  Fragments arranged by size: 

                1029     243     114      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     390    1288    1413 
   Size:        390     898     125

  Fragments arranged by size: 

                 898     390     125
 
TatI w'GTAC_w

Cuts at:      0     851    1413 
   Size:        851     562
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     300     332     965     997    1413 
   Size:        300      32     633      32     416

  Fragments arranged by size: 

                 633     416     300      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1108    1413 
   Size:       1108     305
 
XmnI GAAnn'nnTTC

Cuts at:      0     190     766    1413 
   Size:        190     576     647

  Fragments arranged by size: 

                 647     576     190
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BclI    BfrBI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MmeI     MscI     MslI     NaeI     NarI   NgoMIV     NsiI
     NspI     PfoI    PpuMI     PsiI   PspOMI     PstI     SacI    SanDI
     ScaI     SfcI     SfoI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI     BglI    BglII
     BlpI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI    EcoRI     FseI     FspI    FspAI     KpnI
     MfeI     MluI   MspA1I     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     XbaI     XcmI     XhoI     XmaI     ZraI
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