Restriction map for pF1KB6242
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     AvaI        
AviII       AvrII       BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspEI       BspHI       
BspLU11I    BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        DraII       DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        RcaI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1754  GGTACC               G^GTACC
AcyI         1711  GACGCC               G[GA]^CG[CT]C
AhdI         1948  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1480  GTGCAC               G^TGCAC
AlwNI         454  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1480  GTGCAC               G^TGCAC
Asp718I      1754  GGTACC               G^GTACC
AspEI        1948  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI         773  GTATCC               GTATCC(6/5)
BclI         1175  TGATCA               T^GATCA
BglII        1491  AGATCT               A^GATCT
BsaBI        1141  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1711  GACGCC               G[GA]^CG[CT]C
BsaWI        1541  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     1205  TGTACA               T^GTACA
BspDI         212  ATCGAT               AT^CGAT
BspMI        1074  ACCTGC               ACCTGC(4/8)
BsrDI         267  CATTGC               GCAATG(2/0)
BsrGI        1205  TGTACA               T^GTACA
BstAPI       1117  GCAATTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        880  GGTAACC              G^GT[ACGT]ACC
BstPI         880  GGTAACC              G^GT[ACGT]ACC
ClaI          212  ATCGAT               AT^CGAT
CpoI         1538  CGGACCG              CG^G[AT]CCG
Eam1105I     1948  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         813  CCTTGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       880  GGTAACC              G^GT[ACGT]ACC
FauI          115  GCGGG                CCCGC(4/6)
FbaI         1175  TGATCA               T^GATCA
HgaI         1711  GACGC                GACGC(5/10)
Hin1I        1711  GACGCC               G[GA]^CG[CT]C
HincII        188  GTCAAC               GT[CT]^[GA]AC
HindII        188  GTCAAC               GT[CT]^[GA]AC
KpnI         1754  GGTACC               GGTAC^C
MaeII        1587  ACGT                 A^CGT
MamI         1141  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       1519  CAGCTG               C[AC]G^C[GT]G
NaeI         1811  GCCGGC               GCC^GGC
NgoMIV       1811  GCCGGC               G^CCGGC
PvuII        1519  CAGCTG               CAG^CTG
RsrII        1538  CGGACCG              CG^G[AT]CCG
SacI          577  GAGCTC               GAGCT^C
SmlI         1943  CTCAAG               C^T[CT][GA]AG
SspBI        1205  TGTACA               T^GTACA
StuI          446  AGGCCT               AGG^CCT
XcmI          464  CCAGCGAGACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1050  GTCTAC               GT^[AC][GT]AC
             1956  GTCTAC              
AspI          119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1696  GACCTCGTC           
BalI          323  TGGCCA               TGG^CCA
              461  TGGCCA              
BanI           33  GGTGCC               G^G[CT][GA]CC
             1754  GGTACC              
BanII         577  GAGCTC               G[GA]GC[CT]^C
             1776  GAGCCC              
BcnI          155  CCGGG                CC^[GC]GG
              254  CCCGG               
BsgI         1787  CTGCAC               GTGCAG(16/14)
             1487  GTGCAG              
BsrFI         884  ACCGGC               [GA]^CCGG[CT]
             1811  GCCGGC              
Cfr10I        884  ACCGGC               [GA]^CCGG[CT]
             1811  GCCGGC              
DsaI           29  CCACGG               C^C[GA][CT]GG
             1631  CCGTGG              
EaeI          323  TGGCCA               [CT]^GGCC[GA]
              461  TGGCCA              
EarI         1987  GAAGAG               CTCTTC(1/4)
             2008  GAAGAG              
Ksp632I      1987  GAAGAG               CTCTTC(1/4)
             2008  GAAGAG              
MluNI         323  TGGCCA               TGG^CCA
              461  TGGCCA              
MscI          323  TGGCCA               TGG^CCA
              461  TGGCCA              
NciI          155  CCGGG                CC^[GC]GG
              254  CCCGG               
PflFI         119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1696  GACCTCGTC           
PstI         1034  CTGCAG               CTGCA^G
             1917  CTGCAG              
SfaNI         564  GATGC                GCATC(5/9)
              744  GATGC               
Tth111I       119  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1696  GACCTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BamHI       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PshAI       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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