Restriction map for pF1KB6242
 (Linear) (Six-base) MAPSORT of: KIBB6242.seq  Check: 3257  from: 1  to: 2061



KIBB6242.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1083    2061 
   Size:       1083     978
 
AccI GT'mk_AC

Cuts at:      0    1051    1957    2061 
   Size:       1051     906     104

  Fragments arranged by size: 

                1051     906     104
 
Acc65I G'GTAC_C

Cuts at:      0    1754    2061 
   Size:       1754     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1246    1453    2045    2061 
   Size:       1246     207     592      16

  Fragments arranged by size: 

                1246     592     207      16
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1953    2061 
   Size:       1953     108
 
AleI CACnn'nnGTG

Cuts at:      0     402    2061 
   Size:        402    1659
 
AlwNI CAG_nnn'CTG

Cuts at:      0     459    2061 
   Size:        459    1602
 
ApaLI G'TGCA_C

Cuts at:      0    1480    2061 
   Size:       1480     581
 
ApoI r'AATT_y

Cuts at:      0     174     668     947    1290    1831    1964    2061 
   Size:        174     494     279     343     541     133      97

  Fragments arranged by size: 

                 541     494     343     279     174     133      97
 
BamHI G'GATC_C

Cuts at:      0      15     132     151    2061 
   Size:         15     117      19    1910

  Fragments arranged by size: 

                1910     117      19      15
 
BanI G'GyrC_C

Cuts at:      0      33    1754    2061 
   Size:         33    1721     307

  Fragments arranged by size: 

                1721     307      33
 
BanII G_rGCy'C

Cuts at:      0     581    1780    2061 
   Size:        581    1199     281

  Fragments arranged by size: 

                1199     581     281
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     841     995    1396    1913    2061 
   Size:        841     154     401     517     148

  Fragments arranged by size: 

                 841     517     401     154     148
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     784    2061 
   Size:        784    1277
 
BclI T'GATC_A

Cuts at:      0    1175    2061 
   Size:       1175     886
 
BglII A'GATC_T

Cuts at:      0    1491    2061 
   Size:       1491     570
 
Bme1580I G_kGCm'C

Cuts at:      0    1484    2061 
   Size:       1484     577
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     561    1799    2061 
   Size:        561    1238     262

  Fragments arranged by size: 

                1238     561     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1445    1501    2061 
   Size:        496     949      56     560

  Fragments arranged by size: 

                 949     560     496      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     314    2061 
   Size:        314    1747
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1928    2061 
   Size:       1928     133
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     463     502    1565    2061 
   Size:        463      39    1063     496

  Fragments arranged by size: 

                1063     496     463      39
 
BsaBI GATnn'nnATC

Cuts at:      0    1145    2061 
   Size:       1145     916
 
BsaHI Gr'CG_yC

Cuts at:      0    1712    2061 
   Size:       1712     349
 
BsaWI w'CCGG_w

Cuts at:      0    1541    2061 
   Size:       1541     520
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      17      47    1075    1105    2061 
   Size:         17      30    1028      30     956

  Fragments arranged by size: 

                1028     956      30      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     252     457     493    1567    1819    2005    2061 
   Size:        252     205      36    1074     252     186      56

  Fragments arranged by size: 

                1074     252     252     205     186      56      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1508    1772    2061 
   Size:       1508     264     289

  Fragments arranged by size: 

                1508     289     264
 
BsiHKAI G_wGCw'C

Cuts at:      0     384     581    1484    1765    2061 
   Size:        384     197     903     281     296

  Fragments arranged by size: 

                 903     384     296     281     197
 
Bsp1286I G_dGCh'C

Cuts at:      0     384     581    1484    1765    1780    2061 
   Size:        384     197     903     281      15     281

  Fragments arranged by size: 

                 903     384     281     281     197      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1083    2061 
   Size:       1083     978
 
BsrDI GCAATG_nn'

Cuts at:      0     266    2061 
   Size:        266    1795
 
BsrFI r'CCGG_y

Cuts at:      0     884    1811    2061 
   Size:        884     927     250

  Fragments arranged by size: 

                 927     884     250
 
BsrGI T'GTAC_A

Cuts at:      0    1205    2061 
   Size:       1205     856
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1123    2061 
   Size:       1123     938
 
BstEII G'GTnAC_C

Cuts at:      0     880    2061 
   Size:        880    1181
 
BstYI r'GATC_y

Cuts at:      0      15     132     151    1491    2061 
   Size:         15     117      19    1340     570

  Fragments arranged by size: 

                1340     570     117      19      15
 
BtgI C'CryG_G

Cuts at:      0      29    1631    2061 
   Size:         29    1602     430

  Fragments arranged by size: 

                1602     430      29
 
BtsI GCAGTG_nn'

Cuts at:      0    1926    2061 
   Size:       1926     135
 
ClaI AT'CG_AT

Cuts at:      0     213    2061 
   Size:        213    1848
 
DraI TTT'AAA

Cuts at:      0     558     945    1254    2061 
   Size:        558     387     309     807

  Fragments arranged by size: 

                 807     558     387     309
 
EaeI y'GGCC_r

Cuts at:      0     323     461    2061 
   Size:        323     138    1600

  Fragments arranged by size: 

                1600     323     138
 
EarI CTCTTCn'nnn_

Cuts at:      0    1982    2003    2061 
   Size:       1982      21      58

  Fragments arranged by size: 

                1982      58      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1552    2061 
   Size:       1552     509
 
EcoICRI GAG'CTC

Cuts at:      0     579    2061 
   Size:        579    1482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1246    1445    1453    1501    2045    2061 
   Size:        496     750     199       8      48     544      16

  Fragments arranged by size: 

                 750     544     496     199      48      16       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     817    2061 
   Size:        817    1244
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     829    1331    2061 
   Size:        829     502     730

  Fragments arranged by size: 

                 829     730     502
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     555     587     616     648    1852    1884 
   Size:        288      32     235      32      29      32    1204      32

Cuts at:   1884    1939    1971    2061 
   Size:         55      32      90

  Fragments arranged by size: 

                1204     288     235      90      55      32      32      32
                  32      32      29
 
HincII GTy'rAC

Cuts at:      0     190    2061 
   Size:        190    1871
 
KpnI G_GTAC'C

Cuts at:      0    1758    2061 
   Size:       1758     303
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     572     703    2061 
   Size:        242     330     131    1358

  Fragments arranged by size: 

                1358     330     242     131
 
MscI TGG'CCA

Cuts at:      0     325     463    2061 
   Size:        325     138    1598

  Fragments arranged by size: 

                1598     325     138
 
MslI CAynn'nnrTG

Cuts at:      0     287     402     998    1004    1154    2061 
   Size:        287     115     596       6     150     907

  Fragments arranged by size: 

                 907     596     287     150     115       6
 
MspA1I CmG'CkG

Cuts at:      0    1521    2061 
   Size:       1521     540
 
NaeI GCC'GGC

Cuts at:      0    1813    2061 
   Size:       1813     248
 
NgoMIV G'CCGG_C

Cuts at:      0    1811    2061 
   Size:       1811     250
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     459     784    1239    2061 
   Size:        459     325     455     822

  Fragments arranged by size: 

                 822     459     455     325
 
PfoI T'CCnGG_A

Cuts at:      0     253    2061 
   Size:        253    1808
 
PshAI GACnn'nnGTC

Cuts at:      0     198     491     714    2061 
   Size:        198     293     223    1347

  Fragments arranged by size: 

                1347     293     223     198
 
PstI C_TGCA'G

Cuts at:      0    1038    1921    2061 
   Size:       1038     883     140

  Fragments arranged by size: 

                1038     883     140
 
PvuII CAG'CTG

Cuts at:      0    1521    2061 
   Size:       1521     540
 
RsrII CG'GwC_CG

Cuts at:      0    1539    2061 
   Size:       1539     522
 
SacI G_AGCT'C

Cuts at:      0     581    2061 
   Size:        581    1480
 
SfcI C'TryA_G

Cuts at:      0      84     514    1034    1917    2061 
   Size:         84     430     520     883     144

  Fragments arranged by size: 

                 883     520     430     144      84
 
SmlI C'TyrA_G

Cuts at:      0    1943    2061 
   Size:       1943     118
 
StuI AGG'CCT

Cuts at:      0     448    2061 
   Size:        448    1613
 
StyI C'CwwG_G

Cuts at:      0     128     778     818    1566    2061 
   Size:        128     650      40     748     495

  Fragments arranged by size: 

                 748     650     495     128      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     414     593     598    1589    2061 
   Size:        414     179       5     991     472

  Fragments arranged by size: 

                 991     472     414     179       5
 
TatI w'GTAC_w

Cuts at:      0    1205    1939    2061 
   Size:       1205     734     122

  Fragments arranged by size: 

                1205     734     122
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49     602     634    1612    1644    2061 
   Size:         17      32     553      32     978      32     417

  Fragments arranged by size: 

                 978     553     417      32      32      32      17
 
Tth111I GACn'n_nGTC

Cuts at:      0     122    1699    2061 
   Size:        122    1577     362

  Fragments arranged by size: 

                1577     362     122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    2061 
   Size:        471    1590
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI    AlwNI    ApaLI
     ApoI    BamHI     BanI    BanII     BbsI    BciVI     BclI    BglII
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI   BstEII    BstYI     BtgI     BtsI     ClaI
     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI     FalI
    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV    PflMI     PfoI    PshAI     PstI    PvuII    RsrII     SacI
     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
    BsaAI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
    CspCI   DraIII     DrdI     EagI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI
     XmaI     XmnI     ZraI
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