Restriction map for pF1KE0783
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MboII       MluI        
MseI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TaqI        TfiI        
Tru9I       TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         347  CGCG                 CG^CG
AccIII        707  TCCGGA               T^CCGGA
AcyI          307  GACGCC               G[GA]^CG[CT]C
Alw44I         55  GTGCAC               G^TGCAC
AocI          466  CCTGAGG              CC^T[ACGT]AGG
ApaLI          55  GTGCAC               G^TGCAC
AspI           85  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          809  TGGCCA               TGG^CCA
BanII         944  GAGCCC               G[GA]GC[CT]^C
BcgI          438  CGAGGAAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI         307  GACGCC               G[GA]^CG[CT]C
BsaI          592  GAGACC               GGTCTC(1/5)
BseAI         707  TCCGGA               T^CCGGA
BsmAI         592  GAGAC                GTCTC(1/5)
BspEI         707  TCCGGA               T^CCGGA
BspHI         269  TCATGA               T^CATGA
BstAPI        224  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         347  CGCG                 CG^CG
BstXI         425  CCAACGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        466  CCTGAGG              CC^T[ACGT]AGG
CfoI          348  GCGC                 GCG^C
Eco81I        466  CCTGAGG              CC^T[ACGT]AGG
HgaI          307  GACGC                GACGC(5/10)
HhaI          348  GCGC                 GCG^C
Hin1I         307  GACGCC               G[GA]^CG[CT]C
HinP1I        348  GCGC                 G^CGC
MaeII         395  ACGT                 A^CGT
MfeI          338  CAATTG               C^AATTG
MluNI         809  TGGCCA               TGG^CCA
MroI          707  TCCGGA               T^CCGGA
MscI          809  TGGCCA               TGG^CCA
MunI          338  CAATTG               C^AATTG
MvnI          347  CGCG                 CG^CG
NcoI          105  CCATGG               C^CATGG
PflFI          85  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          372  CTGCAG               CTGCA^G
RcaI          269  TCATGA               T^CATGA
SbfI          371  CCTGCAGG             CCTGCA^GG
SfaNI         127  GATGC                GCATC(5/9)
SfcI          372  CTGCAG               C^T[GA][CT]AG
SmaI          277  CCCGGG               CCC^GGG
SmlI          117  CTCAAG               C^T[CT][GA]AG
Sse8387I      371  CCTGCAGG             CCTGCA^GG
StuI          473  AGGCCT               AGG^CCT
Tsp509I       339  AATT                 ^AATT
Tth111I        85  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           37  CCACGGCTGGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         277  CCCGGG               C^CCGGG
XmaI          277  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI         1020  GTAC                 GT^AC
             1050  GTAC                
AspHI          55  GTGCAC               G[AT]GC[AT]^C
              973  GAGCAC              
BanI          843  GGCACC               G^G[CT][GA]CC
              926  GGCACC              
BglI          188  GCCATGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              369  GCCCTGCAGGC         
BpmI          142  CTGGAG               CTGGAG(16/14)
              609  CTGGAG              
BsaWI         707  TCCGGA               [AT]^CCGG[AT]
              739  ACCGGA              
BsiHKAI        55  GTGCAC               G[AT]GC[AT]^C
              973  GAGCAC              
BstEII        315  GGTCACC              G^GT[ACGT]ACC
              903  GGTGACC             
BstPI         315  GGTCACC              G^GT[ACGT]ACC
              903  GGTGACC             
DrdI          307  GACGCCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              853  GACAGAAAGGTC        
EaeI          434  TGGCCG               [CT]^GGCC[GA]
              809  TGGCCA              
EcoO65I       315  GGTCACC              G^GT[ACGT]ACC
              903  GGTGACC             
EcoT14I       105  CCATGG               C^C[AT][AT]GG
              548  CCAAGG              
FauI          388  CCCGC                CCCGC(4/6)
              596  CCCGC               
HinfI         145  GAGTC                G^A[ACGT]TC
              961  GAGTC               
PflMI         234  CCATTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              978  CCAGGGCTTGG         
PleI          145  GAGTC                GAGTC(4/5)
              961  GAGTC               
PpuMI          89  GGGTCCC              [GA]G^G[AT]CC[CT]
              860  AGGTCCC             
RsaI         1020  GTAC                 GT^AC
             1050  GTAC                
StyI          105  CCATGG               C^C[AT][AT]GG
              548  CCAAGG              
Van91I        234  CCATTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              978  CCAGGGCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaI        
AvaII       BamHI       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeIII      MboI        MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PvuII       Sau3AI      Sau96I      ScrFI       TseI        
Tsp45I      TspRI       XhoII       
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