Restriction map for pF1KE0783
 (Linear) (Six-base) MAPSORT of: KIEE0783.seq  Check: 6026  from: 1  to: 1056



KIEE0783.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     373    1056 
   Size:        373     683
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     230     733     994    1056 
   Size:        156      74     503     261      62

  Fragments arranged by size: 

                 503     261     156      74      62
 
ApaLI G'TGCA_C

Cuts at:      0      55    1056 
   Size:         55    1001
 
AvaI C'yCGr_G

Cuts at:      0      94     277     631     777    1056 
   Size:         94     183     354     146     279

  Fragments arranged by size: 

                 354     279     183     146      94
 
BamHI G'GATC_C

Cuts at:      0     160     384     768     798    1056 
   Size:        160     224     384      30     258

  Fragments arranged by size: 

                 384     258     224     160      30
 
BanI G'GyrC_C

Cuts at:      0     843     926    1056 
   Size:        843      83     130

  Fragments arranged by size: 

                 843     130      83
 
BanII G_rGCy'C

Cuts at:      0     948    1056 
   Size:        948     108
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     427     461    1056 
   Size:        427      34     595

  Fragments arranged by size: 

                 595     427      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     194     375    1056 
   Size:        194     181     681

  Fragments arranged by size: 

                 681     194     181
 
Bme1580I G_kGCm'C

Cuts at:      0      59    1056 
   Size:         59     997
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     166    1056 
   Size:        166     890
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     492    1056 
   Size:        492     564
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     630    1056 
   Size:        163     467     426

  Fragments arranged by size: 

                 467     426     163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102    1056 
   Size:        102     954
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     586    1056 
   Size:        586     470
 
BsaHI Gr'CG_yC

Cuts at:      0     308    1056 
   Size:        308     748
 
BsaWI w'CCGG_w

Cuts at:      0     707     739    1056 
   Size:        707      32     317

  Fragments arranged by size: 

                 707     317      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     190     220    1056 
   Size:        190      30     836

  Fragments arranged by size: 

                 836     190      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     484     627    1030    1056 
   Size:        484     143     403      26

  Fragments arranged by size: 

                 484     403     143      26
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     977    1056 
   Size:         59     918      79

  Fragments arranged by size: 

                 918      79      59
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     948     977    1056 
   Size:         59     889      29      79

  Fragments arranged by size: 

                 889      79      59      29
 
BspEI T'CCGG_A

Cuts at:      0     707    1056 
   Size:        707     349
 
BspHI T'CATG_A

Cuts at:      0     269    1056 
   Size:        269     787
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     230    1056 
   Size:        230     826
 
BstEII G'GTnAC_C

Cuts at:      0     315     903    1056 
   Size:        315     588     153

  Fragments arranged by size: 

                 588     315     153
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     432    1056 
   Size:        432     624
 
BstYI r'GATC_y

Cuts at:      0     160     384     408     712     768     798    1056 
   Size:        160     224      24     304      56      30     258

  Fragments arranged by size: 

                 304     258     224     160      56      30      24
 
Bsu36I CC'TnA_GG

Cuts at:      0     467    1056 
   Size:        467     589
 
BtgI C'CryG_G

Cuts at:      0      37     105     716    1056 
   Size:         37      68     611     340

  Fragments arranged by size: 

                 611     340      68      37
 
BtsI GCAGTG_nn'

Cuts at:      0     437    1056 
   Size:        437     619
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     313     859    1056 
   Size:        313     546     197

  Fragments arranged by size: 

                 546     313     197
 
EaeI y'GGCC_r

Cuts at:      0     434     809    1056 
   Size:        434     375     247

  Fragments arranged by size: 

                 434     375     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     630    1056 
   Size:        163     467     426

  Fragments arranged by size: 

                 467     426     163
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     730     861    1056 
   Size:         90     640     131     195

  Fragments arranged by size: 

                 640     195     131      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184     760     792    1056 
   Size:        152      32     576      32     264

  Fragments arranged by size: 

                 576     264     152      32      32
 
MfeI C'AATT_G

Cuts at:      0     338    1056 
   Size:        338     718
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     449    1056 
   Size:        449     607
 
MscI TGG'CCA

Cuts at:      0     811    1056 
   Size:        811     245
 
MslI CAynn'nnrTG

Cuts at:      0      64     188     901    1056 
   Size:         64     124     713     155

  Fragments arranged by size: 

                 713     155     124      64
 
MspA1I CmG'CkG

Cuts at:      0      75     227     893    1056 
   Size:         75     152     666     163

  Fragments arranged by size: 

                 666     163     152      75
 
NcoI C'CATG_G

Cuts at:      0     105    1056 
   Size:        105     951
 
PasI CC'CwG_GG

Cuts at:      0     733     910    1045    1056 
   Size:        733     177     135      11

  Fragments arranged by size: 

                 733     177     135      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240     984    1056 
   Size:        240     744      72

  Fragments arranged by size: 

                 744     240      72
 
PfoI T'CCnGG_A

Cuts at:      0    1010    1056 
   Size:       1010      46
 
PpuMI rG'GwC_Cy

Cuts at:      0      90     861    1056 
   Size:         90     771     195

  Fragments arranged by size: 

                 771     195      90
 
PstI C_TGCA'G

Cuts at:      0     376    1056 
   Size:        376     680
 
PvuII CAG'CTG

Cuts at:      0      75     227     893    1056 
   Size:         75     152     666     163

  Fragments arranged by size: 

                 666     163     152      75
 
SanDI GG'GwC_CC

Cuts at:      0      90    1056 
   Size:         90     966
 
SbfI CC_TGCA'GG

Cuts at:      0     376    1056 
   Size:        376     680
 
SfcI C'TryA_G

Cuts at:      0     372    1056 
   Size:        372     684
 
SmaI CCC'GGG

Cuts at:      0     279    1056 
   Size:        279     777
 
SmlI C'TyrA_G

Cuts at:      0     117    1056 
   Size:        117     939
 
StuI AGG'CCT

Cuts at:      0     475    1056 
   Size:        475     581
 
StyI C'CwwG_G

Cuts at:      0     105     548    1056 
   Size:        105     443     508

  Fragments arranged by size: 

                 508     443     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     876    1056 
   Size:        876     180
 
Tth111I GACn'n_nGTC

Cuts at:      0      88    1056 
   Size:         88     968
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      44    1056 
   Size:         44    1012
 
XmaI C'CCGG_G

Cuts at:      0     277    1056 
   Size:        277     779
 

 Enzymes that do cut:

     AlfI    AlwNI    ApaLI     AvaI    BamHI     BanI    BanII     BcgI
     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspEI    BspHI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
  Eco57MI EcoO109I    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     PasI    PflMI     PfoI    PpuMI     PstI    PvuII    SanDI
     SbfI     SfcI     SmaI     SmlI     StuI     StyI    TaqII  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaAI
    BsaBI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI     XbaI     XhoI
     XmnI     ZraI
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