Restriction map for pF1KE0773
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        MluI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccIII         86  TCCGGA               T^CCGGA
AcyI          239  GGCGTC               G[GA]^CG[CT]C
AflIII        269  ACGTGT               A^C[GA][CT]GT
AocI          681  CCTGAGG              CC^T[ACGT]AGG
AvaI          281  CTCGGG               C^[CT]CG[GA]G
AviII         365  TGCGCA               TGC^GCA
BalI          159  TGGCCA               TGG^CCA
BanI          703  GGCACC               G^G[CT][GA]CC
BbsI          690  GAAGAC               GAAGAC(2/6)
BcgI          368  GCAGAGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          981  CCGGG                CC^[GC]GG
BfaI          460  CTAG                 C^TAG
BpuAI         690  GAAGAC               GAAGAC(2/6)
BsaBI         375  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         239  GGCGTC               G[GA]^CG[CT]C
BseAI          86  TCCGGA               T^CCGGA
BsiEI          36  CGGCCG               CG[GA][CT]^CG
BsmBI         415  CGTCTC               CGTCTC(1/5)
BsoBI         281  CTCGGG               C^[CT]CG[GA]G
BspEI          86  TCCGGA               T^CCGGA
BspHI         122  TCATGA               T^CATGA
BsrBI         129  CCGCTC               CCGCTC(-3/-3)
BstAPI        357  GCATCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         533  CCACGATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        681  CCTGAGG              CC^T[ACGT]AGG
EagI           36  CGGCCG               C^GGCCG
EclXI          36  CGGCCG               C^GGCCG
Eco52I         36  CGGCCG               C^GGCCG
Eco81I        681  CCTGAGG              CC^T[ACGT]AGG
FspI          365  TGCGCA               TGC^GCA
HaeII         309  GGCGCT               [GA]GCGC^[CT]
Hin1I         239  GGCGTC               G[GA]^CG[CT]C
KspI          474  CCGCGG               CCGC^GG
MaeI          460  CTAG                 C^TAG
MaeII         269  ACGT                 A^CGT
MaeIII        100  GTCAC                ^GT[ACGT]AC
MamI          375  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          218  CAATTG               C^AATTG
MluNI         159  TGGCCA               TGG^CCA
MroI           86  TCCGGA               T^CCGGA
MscI          159  TGGCCA               TGG^CCA
MseI          202  TTAA                 T^TAA
MunI          218  CAATTG               C^AATTG
NciI          981  CCGGG                CC^[GC]GG
NcoI          573  CCATGG               C^CATGG
PleI           80  GAGTC                GAGTC(4/5)
PstI          496  CTGCAG               CTGCA^G
RcaI          122  TCATGA               T^CATGA
SacI          481  GAGCTC               GAGCT^C
SacII         474  CCGCGG               CCGC^GG
SapI          501  GAAGAGC              GCTCTTC(1/4)
SfcI          496  CTGCAG               C^T[GA][CT]AG
TaqI          377  TCGA                 T^CGA
Tru9I         202  TTAA                 T^TAA
Tsp45I        100  GTCAC                ^GT[GC]AC
Tsp509I       219  AATT                 ^AATT
TspRI         183  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       377  TCGA                 T^CGA
XbaI          459  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII         206  CGCG                 CG^CG
              475  CGCG                
AfaI          273  GTAC                 GT^AC
              567  GTAC                
AlwI          446  GGATC                GGATC(4/5)
              762  GGATC               
AspHI          63  GAGCAC               G[AT]GC[AT]^C
              481  GAGCTC              
BsaI          838  GAGACC               GGTCTC(1/5)
              960  GGTCTC              
BsaWI          86  TCCGGA               [AT]^CCGG[AT]
              437  ACCGGA              
BsiHKAI        63  GAGCAC               G[AT]GC[AT]^C
              481  GAGCTC              
BsmFI         187  GGGAC                GGGAC(10/14)
              330  GTCCC               
BspMI         392  ACCTGC               ACCTGC(4/8)
              716  ACCTGC              
BstF5I        400  CATCC                GGATG(2/0)
              408  CATCC               
BstUI         206  CGCG                 CG^CG
              475  CGCG                
BstYI         446  GGATCT               [GA]^GATC[CT]
              762  GGATCT              
Cac8I         669  GCATGC               GC[ACGT]^[ACGT]GC
              743  GCATGC              
DraII         317  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              902  AGGACCT             
EaeI           36  CGGCCG               [CT]^GGCC[GA]
              159  TGGCCA              
EarI          322  CTCTTC               CTCTTC(1/4)
              501  GAAGAG              
EcoNI         677  CCTCCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              951  CCTGGCCCAGG         
EcoO109I      317  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              902  AGGACCT             
EcoT14I       573  CCATGG               C^C[AT][AT]GG
              973  CCTTGG              
FokI          400  CATCC                GGATG(9/13)
              408  CATCC               
HgaI          240  GCGTC                GACGC(5/10)
              414  GCGTC               
Ksp632I       322  CTCTTC               CTCTTC(1/4)
              501  GAAGAG              
MflI          446  GGATCT               [GA]^GATC[CT]
              762  GGATCT              
MslI          534  CACGATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              774  CATTGGAGTG          
MvnI          206  CGCG                 CG^CG
              475  CGCG                
MwoI          357  GCATCAGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              618  GCTCTTAGGGC         
NspI          669  GCATGC               [GA]CATG^[CT]
              743  GCATGC              
PpuMI         317  AGGTCCT              [GA]G^G[AT]CC[CT]
              902  AGGACCT             
PvuII         149  CAGCTG               CAG^CTG
              361  CAGCTG              
RsaI          273  GTAC                 GT^AC
              567  GTAC                
SmlI          313  CTCAAG               C^T[CT][GA]AG
              628  CTCAAG              
SphI          669  GCATGC               GCATG^C
              743  GCATGC              
StyI          573  CCATGG               C^C[AT][AT]GG
              973  CCTTGG              
TfiI          456  GATTC                G^A[AT]TC
              588  GATTC               
XhoII         446  GGATCT               [GA]^GATC[CT]
              762  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BmyI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstNI       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MnlI        MspA1I      
MspI        MvaI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
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