Restriction map for pF1KE0773
 (Linear) (Six-base) MAPSORT of: KIEE0773.seq  Check: 7459  from: 1  to: 1032



KIEE0773.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     401    1032 
   Size:        401     631
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1032 
   Size:        661     371
 
AflIII A'CryG_T

Cuts at:      0     269    1032 
   Size:        269     763
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     796    1032 
   Size:        796     236
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     856     888    1032 
   Size:        856      32     144

  Fragments arranged by size: 

                 856     144      32
 
AvaI C'yCGr_G

Cuts at:      0     281    1032 
   Size:        281     751
 
BanI G'GyrC_C

Cuts at:      0     703    1032 
   Size:        703     329
 
BanII G_rGCy'C

Cuts at:      0     485     629     900    1032 
   Size:        485     144     271     132

  Fragments arranged by size: 

                 485     271     144     132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     697    1032 
   Size:        697     335
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357     391    1032 
   Size:        357      34     641

  Fragments arranged by size: 

                 641     357      34
 
Bme1580I G_kGCm'C

Cuts at:      0     890    1032 
   Size:        890     142
 
Bpu10I CC'TnA_GC

Cuts at:      0      61     922    1032 
   Size:         61     861     110

  Fragments arranged by size: 

                 861     110      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     613    1032 
   Size:        298     315     419

  Fragments arranged by size: 

                 419     315     298
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     832     966    1032 
   Size:        832     134      66

  Fragments arranged by size: 

                 832     134      66
 
BsaBI GATnn'nnATC

Cuts at:      0     379    1032 
   Size:        379     653
 
BsaHI Gr'CG_yC

Cuts at:      0     240    1032 
   Size:        240     792
 
BsaWI w'CCGG_w

Cuts at:      0      86     437    1032 
   Size:         86     351     595

  Fragments arranged by size: 

                 595     351      86
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     895     925    1032 
   Size:        895      30     107

  Fragments arranged by size: 

                 895     107      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     456     559     699    1032 
   Size:        456     103     140     333

  Fragments arranged by size: 

                 456     333     140     103
 
BseYI C'CCAG_C

Cuts at:      0      74    1032 
   Size:         74     958
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     379     492    1032 
   Size:         95     284     113     540

  Fragments arranged by size: 

                 540     284     113      95
 
BsiEI CG_ry'CG

Cuts at:      0      39    1032 
   Size:         39     993
 
BsiHKAI G_wGCw'C

Cuts at:      0      67     485    1032 
   Size:         67     418     547

  Fragments arranged by size: 

                 547     418      67
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     421    1032 
   Size:        421     611
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     485     629     890     900    1032 
   Size:         67     418     144     261      10     132

  Fragments arranged by size: 

                 418     261     144     132      67      10
 
BspEI T'CCGG_A

Cuts at:      0      86    1032 
   Size:         86     946
 
BspHI T'CATG_A

Cuts at:      0     122    1032 
   Size:        122     910
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     725    1032 
   Size:        401     324     307

  Fragments arranged by size: 

                 401     324     307
 
BsrBI CCG'CTC

Cuts at:      0     131    1032 
   Size:        131     901
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     363    1032 
   Size:        363     669
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     540    1032 
   Size:        540     492
 
BstYI r'GATC_y

Cuts at:      0     446     762    1032 
   Size:        446     316     270

  Fragments arranged by size: 

                 446     316     270
 
Bsu36I CC'TnA_GG

Cuts at:      0     682    1032 
   Size:        682     350
 
BtgI C'CryG_G

Cuts at:      0     474     573     890    1032 
   Size:        474      99     317     142

  Fragments arranged by size: 

                 474     317     142      99
 
BtsI GCAGTG_nn'

Cuts at:      0     189    1032 
   Size:        189     843
 
EaeI y'GGCC_r

Cuts at:      0      36     159    1032 
   Size:         36     123     873

  Fragments arranged by size: 

                 873     123      36
 
EagI C'GGCC_G

Cuts at:      0      36    1032 
   Size:         36     996
 
EarI CTCTTCn'nnn_

Cuts at:      0     328     496    1032 
   Size:        328     168     536

  Fragments arranged by size: 

                 536     328     168
 
EcoICRI GAG'CTC

Cuts at:      0     483    1032 
   Size:        483     549
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1032 
   Size:        661     371
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     681     955    1032 
   Size:        681     274      77

  Fragments arranged by size: 

                 681     274      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     318     903    1032 
   Size:        318     585     129

  Fragments arranged by size: 

                 585     318     129
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     339    1032 
   Size:        339     693
 
FspI TGC'GCA

Cuts at:      0     367    1032 
   Size:        367     665
 
HaeII r_GCGC'y

Cuts at:      0     313    1032 
   Size:        313     719
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     225     257     811     843     895     927    1032 
   Size:        225      32     554      32      52      32     105

  Fragments arranged by size: 

                 554     225     105      52      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     218    1032 
   Size:        218     814
 
MscI TGG'CCA

Cuts at:      0     161    1032 
   Size:        161     871
 
MslI CAynn'nnrTG

Cuts at:      0     538     778    1032 
   Size:        538     240     254

  Fragments arranged by size: 

                 538     254     240
 
MspA1I CmG'CkG

Cuts at:      0     151     363     476    1032 
   Size:        151     212     113     556

  Fragments arranged by size: 

                 556     212     151     113
 
NcoI C'CATG_G

Cuts at:      0     573    1032 
   Size:        573     459
 
NspI r_CATG'y

Cuts at:      0     673     747    1032 
   Size:        673      74     285

  Fragments arranged by size: 

                 673     285      74
 
PpuMI rG'GwC_Cy

Cuts at:      0     318     903    1032 
   Size:        318     585     129

  Fragments arranged by size: 

                 585     318     129
 
PstI C_TGCA'G

Cuts at:      0     500    1032 
   Size:        500     532
 
PvuII CAG'CTG

Cuts at:      0     151     363    1032 
   Size:        151     212     669

  Fragments arranged by size: 

                 669     212     151
 
SacI G_AGCT'C

Cuts at:      0     485    1032 
   Size:        485     547
 
SacII CC_GC'GG

Cuts at:      0     477    1032 
   Size:        477     555
 
SapI GCTCTTCn'nnn_

Cuts at:      0     496    1032 
   Size:        496     536
 
SfcI C'TryA_G

Cuts at:      0     496    1032 
   Size:        496     536
 
SmlI C'TyrA_G

Cuts at:      0     313     628    1032 
   Size:        313     315     404

  Fragments arranged by size: 

                 404     315     313
 
SphI G_CATG'C

Cuts at:      0     673     747    1032 
   Size:        673      74     285

  Fragments arranged by size: 

                 673     285      74
 
StyI C'CwwG_G

Cuts at:      0     573     973    1032 
   Size:        573     400      59

  Fragments arranged by size: 

                 573     400      59
 
TatI w'GTAC_w

Cuts at:      0     272     566    1032 
   Size:        272     294     466

  Fragments arranged by size: 

                 466     294     272
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     754     786    1032 
   Size:        754      32     246

  Fragments arranged by size: 

                 754     246      32
 
XbaI T'CTAG_A

Cuts at:      0     459    1032 
   Size:        459     573
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI     AvaI     BanI    BanII
     BbsI     BcgI Bme1580I   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I     MfeI     MscI
     MslI   MspA1I     NcoI     NspI    PpuMI     PstI    PvuII     SacI
    SacII     SapI     SfcI     SmlI     SphI     StyI     TatI     TstI
     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
    BsaAI    BsiWI     BsmI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI
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