Restriction map for pF1KB5975
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tsp45I      
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          568  GTCTAC               GT^[AC][GT]AC
AccII          18  CGCG                 CG^CG
AccIII        131  TCCGGA               T^CCGGA
AcsI         1234  GAATTT               [GA]^AATT[CT]
AfaI         1146  GTAC                 GT^AC
AflIII        274  ACATGT               A^C[GA][CT]GT
AocI         1445  CCTCAGG              CC^T[ACGT]AGG
ApoI         1234  GAATTT               [GA]^AATT[CT]
AspHI        1015  GAGCTC               G[AT]GC[AT]^C
AvaII        1307  GGACC                G^G[AT]CC
AvrII         420  CCTAGG               C^CTAGG
BalI          320  TGGCCA               TGG^CCA
BanI          897  GGCACC               G^G[CT][GA]CC
BbsI         1416  GTCTTC               GAAGAC(2/6)
BbvCI           4  GCTGAGG              CCTCAGC(-5/-2)
BcnI           62  CCCGG                CC^[GC]GG
BglII         325  AGATCT               A^GATCT
BlnI          420  CCTAGG               C^CTAGG
BpuAI        1416  GTCTTC               GAAGAC(2/6)
BsaWI         131  TCCGGA               [AT]^CCGG[AT]
BseAI         131  TCCGGA               T^CCGGA
BseRI           7  GAGGAG               GAGGAG(10/8)
BsgI          278  GTGCAG               GTGCAG(16/14)
BsiHKAI      1015  GAGCTC               G[AT]GC[AT]^C
BsmFI         749  GGGAC                GGGAC(10/14)
Bsp1407I     1145  TGTACA               T^GTACA
BspEI         131  TCCGGA               T^CCGGA
BspLU11I      274  ACATGT               A^CATGT
BsrGI        1145  TGTACA               T^GTACA
BstF5I       1463  CATCC                GGATG(2/0)
BstUI          18  CGCG                 CG^CG
BstXI         887  CCAACTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1445  CCTCAGG              CC^T[ACGT]AGG
DsaI          744  CCACGG               C^C[GA][CT]GG
Eco81I       1445  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       420  CCTAGG               C^C[AT][AT]GG
EcoT22I       959  ATGCAT               ATGCA^T
FokI         1463  CATCC                GGATG(9/13)
HgaI         1437  GACGC                GACGC(5/10)
MaeIII       1109  GTAAC                ^GT[ACGT]AC
MluNI         320  TGGCCA               TGG^CCA
MroI          131  TCCGGA               T^CCGGA
MscI          320  TGGCCA               TGG^CCA
MslI          825  CATTGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           18  CGCG                 CG^CG
NciI           62  CCCGG                CC^[GC]GG
NruI           17  TCGCGA               TCG^CGA
NsiI          959  ATGCAT               ATGCA^T
PflMI         531  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI         1146  GTAC                 GT^AC
SacI         1015  GAGCTC               GAGCT^C
SspBI        1145  TGTACA               T^GTACA
StyI          420  CCTAGG               C^C[AT][AT]GG
TaqI          905  TCGA                 T^CGA
TthHB8I       905  TCGA                 T^CGA
Van91I        531  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          528  GATCC                GGATC(4/5)
             1324  GATCC               
Asp700I       169  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              444  GAAGCAGTTC          
BanII        1015  GAGCTC               G[GA]GC[CT]^C
             1386  GGGCTC              
BglI          148  GCCTGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              712  GCCATCTTGGC         
BmyI         1015  GAGCTC               G[AGT]GC[ACT]^C
             1386  GGGCTC              
Bsp1286I     1015  GAGCTC               G[AGT]GC[ACT]^C
             1386  GGGCTC              
BspMI        1362  ACCTGC               ACCTGC(4/8)
              315  GCAGGT              
BsrDI         677  GCAATG               GCAATG(2/0)
             1411  GCAATG              
EarI          265  CTCTTC               CTCTTC(1/4)
             1487  CTCTTC              
EcoNI         309  CCTCAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1209  CCTACTCAAGG         
HapII          63  CCGG                 C^CGG
              132  CCGG                
HpaII          63  CCGG                 C^CGG
              132  CCGG                
HphI          592  TCACC                GGTGA(8/7)
              767  TCACC               
Ksp632I       265  CTCTTC               CTCTTC(1/4)
             1487  CTCTTC              
MseI          491  TTAA                 T^TAA
             1319  TTAA                
MspA1I        870  CAGCTG               C[AC]G^C[GT]G
             1424  CAGCTG              
MspI           63  CCGG                 C^CGG
              132  CCGG                
NlaIV         897  GGCACC               GG[ACGT]^[ACGT]CC
             1497  GGAGCC              
NspI          274  ACATGT               [GA]CATG^[CT]
             1022  ACATGC              
PvuII         870  CAGCTG               CAG^CTG
             1424  CAGCTG              
SfaNI         735  GATGC                GCATC(5/9)
              961  GCATC               
SmlI          310  CTCAAG               C^T[CT][GA]AG
             1213  CTCAAG              
Tru9I         491  TTAA                 T^TAA
             1319  TTAA                
XmnI          169  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              444  GAAGCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        AlwNI       BbvI        BfaI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        MaeI        
MboI        MboII       MflI        MnlI        MvaI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
Sau96I      ScrFI       TfiI        TseI        Tsp509I     
TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com