Restriction map for pF1KB5975
 (Linear) (Six-base) MAPSORT of: KIBB5975.seq  Check: 641  from: 1  to: 1557



KIBB5975.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     306    1557 
   Size:        306    1251
 
AccI GT'mk_AC

Cuts at:      0     569    1557 
   Size:        569     988
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1403    1557 
   Size:        463     940     154

  Fragments arranged by size: 

                 940     463     154
 
AflIII A'CryG_T

Cuts at:      0     274    1557 
   Size:        274    1283
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     751     974    1557 
   Size:        751     223     583

  Fragments arranged by size: 

                 751     583     223
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      18      50    1557 
   Size:         18      32    1507

  Fragments arranged by size: 

                1507      32      18
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1383    1426    1453    1507    1557 
   Size:       1383      43      27      54      50

  Fragments arranged by size: 

                1383      54      50      43      27
 
ApoI r'AATT_y

Cuts at:      0    1234    1557 
   Size:       1234     323
 
AvrII C'CTAG_G

Cuts at:      0     420    1557 
   Size:        420    1137
 
BanI G'GyrC_C

Cuts at:      0     897    1557 
   Size:        897     660
 
BanII G_rGCy'C

Cuts at:      0    1019    1390    1557 
   Size:       1019     371     167

  Fragments arranged by size: 

                1019     371     167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1409    1557 
   Size:       1409     148
 
BbvCI CC'TCA_GC

Cuts at:      0       5    1557 
   Size:          5    1552
 
BfrBI ATG'CAT

Cuts at:      0     961    1557 
   Size:        961     596
 
BglI GCCn_nnn'nGGC

Cuts at:      0     154     718    1557 
   Size:        154     564     839

  Fragments arranged by size: 

                 839     564     154
 
BglII A'GATC_T

Cuts at:      0     325    1557 
   Size:        325    1232
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1461    1557 
   Size:       1461      96
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1297    1557 
   Size:       1297     260
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     559    1267    1475    1516    1557 
   Size:         62     497     708     208      41      41

  Fragments arranged by size: 

                 708     497     208      62      41      41
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    1557 
   Size:          5    1552
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1198    1557 
   Size:        295     903     359

  Fragments arranged by size: 

                 903     359     295
 
BsaWI w'CCGG_w

Cuts at:      0     131    1557 
   Size:        131    1426
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22    1557 
   Size:         22    1535
 
BseYI C'CCAG_C

Cuts at:      0     519    1557 
   Size:        519    1038
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299    1557 
   Size:        299    1258
 
BsiHKAI G_wGCw'C

Cuts at:      0    1019    1557 
   Size:       1019     538
 
Bsp1286I G_dGCh'C

Cuts at:      0    1019    1390    1557 
   Size:       1019     371     167

  Fragments arranged by size: 

                1019     371     167
 
BspEI T'CCGG_A

Cuts at:      0     131    1557 
   Size:        131    1426
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     306    1371    1557 
   Size:        306    1065     186

  Fragments arranged by size: 

                1065     306     186
 
BsrDI GCAATG_nn'

Cuts at:      0     684    1418    1557 
   Size:        684     734     139

  Fragments arranged by size: 

                 734     684     139
 
BsrGI T'GTAC_A

Cuts at:      0    1145    1557 
   Size:       1145     412
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     894    1557 
   Size:        894     663
 
BstYI r'GATC_y

Cuts at:      0     325     527    1323    1557 
   Size:        325     202     796     234

  Fragments arranged by size: 

                 796     325     234     202
 
Bsu36I CC'TnA_GG

Cuts at:      0    1446    1557 
   Size:       1446     111
 
BtgI C'CryG_G

Cuts at:      0     744    1557 
   Size:        744     813
 
BtsI GCAGTG_nn'

Cuts at:      0     587    1040    1557 
   Size:        587     453     517

  Fragments arranged by size: 

                 587     517     453
 
EaeI y'GGCC_r

Cuts at:      0     320     863     986    1557 
   Size:        320     543     123     571

  Fragments arranged by size: 

                 571     543     320     123
 
EarI CTCTTCn'nnn_

Cuts at:      0     271    1493    1557 
   Size:        271    1222      64

  Fragments arranged by size: 

                1222     271      64
 
EcoICRI GAG'CTC

Cuts at:      0    1017    1557 
   Size:       1017     540
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     463     559    1267    1403    1475    1516    1557 
   Size:         62     401      96     708     136      72      41      41

  Fragments arranged by size: 

                 708     401     136      96      72      62      41      41
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313    1213    1557 
   Size:        313     900     344

  Fragments arranged by size: 

                 900     344     313
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     190     549     732    1557 
   Size:        190     359     183     825

  Fragments arranged by size: 

                 825     359     190     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      12      44    1210    1242    1315    1347    1557 
   Size:         12      32    1166      32      73      32     210

  Fragments arranged by size: 

                1166     210      73      32      32      32      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1549    1557 
   Size:       1549       8
 
MscI TGG'CCA

Cuts at:      0     322    1557 
   Size:        322    1235
 
MslI CAynn'nnrTG

Cuts at:      0     829    1557 
   Size:        829     728
 
MspA1I CmG'CkG

Cuts at:      0     872    1426    1557 
   Size:        872     554     131

  Fragments arranged by size: 

                 872     554     131
 
NruI TCG'CGA

Cuts at:      0      19    1557 
   Size:         19    1538
 
NsiI A_TGCA'T

Cuts at:      0     963    1557 
   Size:        963     594
 
NspI r_CATG'y

Cuts at:      0     278    1026    1557 
   Size:        278     748     531

  Fragments arranged by size: 

                 748     531     278
 
PciI A'CATG_T

Cuts at:      0     274    1557 
   Size:        274    1283
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     537    1557 
   Size:        537    1020
 
PvuII CAG'CTG

Cuts at:      0     872    1426    1557 
   Size:        872     554     131

  Fragments arranged by size: 

                 872     554     131
 
SacI G_AGCT'C

Cuts at:      0    1019    1557 
   Size:       1019     538
 
SmlI C'TyrA_G

Cuts at:      0     310    1213    1557 
   Size:        310     903     344

  Fragments arranged by size: 

                 903     344     310
 
StyI C'CwwG_G

Cuts at:      0     420    1557 
   Size:        420    1137
 
TatI w'GTAC_w

Cuts at:      0    1145    1557 
   Size:       1145     412
 
XmnI GAAnn'nnTTC

Cuts at:      0     173     448    1557 
   Size:        173     275    1109

  Fragments arranged by size: 

                1109     275     173
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AlfI     AloI    AlwNI     ApoI
    AvrII     BanI    BanII     BbsI    BbvCI    BfrBI     BglI    BglII
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI    BsrGI    BstXI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI     FalI    Hin4I     MmeI     MscI     MslI   MspA1I     NruI
     NsiI     NspI     PciI    PflMI    PvuII     SacI     SmlI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BbeI     BcgI    BciVI     BclI     BlpI Bme1580I    BmgBI     BmtI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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