Restriction map for pF1KB5895
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbvCI       BciVI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KpnI        MfeI        
MluI        MluNI       MscI        MslI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NspI        PacI        PaeR7I      PinAI       PmeI        
PshBI       Psp1406I    PstI        PvuII       RsrII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII        204  TCCGGA               T^CCGGA
AcsI         1154  AAATTC               [GA]^AATT[CT]
AcyI         1095  GACGCC               G[GA]^CG[CT]C
AfaI          498  GTAC                 GT^AC
AlwNI         928  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1154  AAATTC               [GA]^AATT[CT]
Asp700I       915  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1291  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          671  CCCGGG               C^[CT]CG[GA]G
BbrPI         514  CACGTG               CAC^GTG
BsaAI         514  CACGTG               [CT]AC^GT[GA]
BsaHI        1095  GACGCC               G[GA]^CG[CT]C
BseAI         204  TCCGGA               T^CCGGA
BseRI         234  CTCCTC               GAGGAG(10/8)
BsiEI         841  CGATCG               CG[GA][CT]^CG
BsmBI        1093  GAGACG               CGTCTC(1/5)
BsoBI         671  CCCGGG               C^[CT]CG[GA]G
BspEI         204  TCCGGA               T^CCGGA
BspHI        1241  TCATGA               T^CATGA
BssSI         835  CACGAG               CACGAG(-5/-1)
BstBI         191  TTCGAA               TT^CGAA
EaeI          311  TGGCCG               [CT]^GGCC[GA]
EcoRV        1225  GATATC               GAT^ATC
EcoT22I        42  ATGCAT               ATGCA^T
HgaI         1095  GACGC                GACGC(5/10)
Hin1I        1095  GACGCC               G[GA]^CG[CT]C
MroI          204  TCCGGA               T^CCGGA
MseI         1066  TTAA                 T^TAA
NcoI         1312  CCATGG               C^CATGG
NsiI           42  ATGCAT               ATGCA^T
NspV          191  TTCGAA               TT^CGAA
PflFI        1291  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1280  CCAATGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         514  CACGTG               CAC^GTG
PmlI          514  CACGTG               CAC^GTG
PpuMI         588  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI           4  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          841  CGATCG               CGAT^CG
RcaI         1241  TCATGA               T^CATGA
RsaI          498  GTAC                 GT^AC
SfcI          777  CTACAG               C^T[GA][CT]AG
SfuI          191  TTCGAA               TT^CGAA
SmaI          671  CCCGGG               CCC^GGG
SmlI          107  CTCAAG               C^T[CT][GA]AG
StuI         1338  AGGCCT               AGG^CCT
Tru9I        1066  TTAA                 T^TAA
Tth111I      1291  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1280  CCAATGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         671  CCCGGG               C^CCGGG
XmaI          671  CCCGGG               C^CCGGG
XmnI          915  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Alw44I        509  GTGCAC               G^TGCAC
              847  GTGCAC              
ApaLI         509  GTGCAC               G^TGCAC
              847  GTGCAC              
AvaII         589  GGACC                G^G[AT]CC
             1296  GGTCC               
BbsI          144  GAAGAC               GAAGAC(2/6)
             1350  GAAGAC              
BclI         1238  TGATCA               T^GATCA
             1244  TGATCA              
BcnI          671  CCCGG                CC^[GC]GG
              672  CCGGG               
BglI          413  GCCTTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1331  GCCACTCAGGC         
BpmI          629  CTCCAG               CTGGAG(16/14)
              451  CTGGAG              
BpuAI         144  GAAGAC               GAAGAC(2/6)
             1350  GAAGAC              
BsaWI         204  TCCGGA               [AT]^CCGG[AT]
              443  TCCGGT              
BsgI          871  CTGCAC               GTGCAG(16/14)
             1268  GTGCAG              
BsmAI        1093  GAGAC                GTCTC(1/5)
              324  GTCTC               
BstYI         296  GGATCT               [GA]^GATC[CT]
             1070  AGATCC              
CfoI          316  GCGC                 GCG^C
             1329  GCGC                
DraII         588  GGGACCT              [GA]G^G[ACGT]CC[CT]
              929  AGGGCCT             
EcoO109I      588  GGGACCT              [GA]G^G[ACGT]CC[CT]
              929  AGGGCCT             
EcoT14I        66  CCTTGG               C^C[AT][AT]GG
             1312  CCATGG              
FauI          410  CCCGC                CCCGC(4/6)
              586  GCGGG               
FbaI         1238  TGATCA               T^GATCA
             1244  TGATCA              
HhaI          316  GCGC                 GCG^C
             1329  GCGC                
HinP1I        316  GCGC                 G^CGC
             1329  GCGC                
KspI          584  CCGCGG               CCGC^GG
              994  CCGCGG              
MflI          296  GGATCT               [GA]^GATC[CT]
             1070  AGATCC              
NciI          671  CCCGG                CC^[GC]GG
              672  CCGGG               
PleI          862  GACTC                GAGTC(4/5)
             1361  GAGTC               
SacI          104  GAGCTC               GAGCT^C
              772  GAGCTC              
SacII         584  CCGCGG               CCGC^GG
              994  CCGCGG              
StyI           66  CCTTGG               C^C[AT][AT]GG
             1312  CCATGG              
Tsp45I        908  GTGAC                ^GT[GC]AC
             1122  GTCAC               
XhoII         296  GGATCT               [GA]^GATC[CT]
             1070  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AflIII      AluI        AlwI        
AspHI       BanII       BbvI        BcgI        BfaI        
BmyI        BsaBI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NdeII       NgoMIV      NlaIII      NlaIV       SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        TseI        Tsp509I     TspRI       TthHB8I     
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