Restriction map for pF1KB5895
 (Linear) (Six-base) MAPSORT of: KIBB5895.seq  Check: 4772  from: 1  to: 1497



KIBB5895.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     906    1369    1497 
   Size:        340     566     463     128

  Fragments arranged by size: 

                 566     463     340     128
 
AflIII A'CryG_T

Cuts at:      0     209     513     515    1497 
   Size:        209     304       2     982

  Fragments arranged by size: 

                 982     304     209       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     933    1497 
   Size:        933     564
 
ApaLI G'TGCA_C

Cuts at:      0     509     847    1497 
   Size:        509     338     650

  Fragments arranged by size: 

                 650     509     338
 
ApoI r'AATT_y

Cuts at:      0    1154    1497 
   Size:       1154     343
 
AvaI C'yCGr_G

Cuts at:      0     671    1497 
   Size:        671     826
 
BanII G_rGCy'C

Cuts at:      0     108     745     776    1035    1497 
   Size:        108     637      31     259     462

  Fragments arranged by size: 

                 637     462     259     108      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     151    1357    1497 
   Size:        151    1206     140

  Fragments arranged by size: 

                1206     151     140
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     411     445     454     488     522     556    1497 
   Size:        411      34       9      34      34      34     941

  Fragments arranged by size: 

                 941     411      34      34      34      34       9
 
BclI T'GATC_A

Cuts at:      0    1238    1244    1497 
   Size:       1238       6     253

  Fragments arranged by size: 

                1238     253       6
 
BfrBI ATG'CAT

Cuts at:      0      44    1497 
   Size:         44    1453
 
BglI GCCn_nnn'nGGC

Cuts at:      0     419    1337    1497 
   Size:        419     918     160

  Fragments arranged by size: 

                 918     419     160
 
Bme1580I G_kGCm'C

Cuts at:      0      66     513     612     851    1497 
   Size:         66     447      99     239     646

  Fragments arranged by size: 

                 646     447     239      99      66
 
BmgBI CAC'GTC

Cuts at:      0     981    1497 
   Size:        981     516
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1029    1497 
   Size:       1029     468
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     614    1497 
   Size:        472     142     883

  Fragments arranged by size: 

                 883     472     142
 
Bpu10I CC'TnA_GC

Cuts at:      0     688     940    1497 
   Size:        688     252     557

  Fragments arranged by size: 

                 688     557     252
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1497 
   Size:         92    1405
 
BsaAI yAC'GTr

Cuts at:      0     516    1497 
   Size:        516     981
 
BsaBI GATnn'nnATC

Cuts at:      0     193     256    1243    1497 
   Size:        193      63     987     254

  Fragments arranged by size: 

                 987     254     193      63
 
BsaHI Gr'CG_yC

Cuts at:      0    1096    1497 
   Size:       1096     401
 
BsaWI w'CCGG_w

Cuts at:      0     204     443    1497 
   Size:        204     239    1054

  Fragments arranged by size: 

                1054     239     204
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     727     757    1497 
   Size:        727      30     740

  Fragments arranged by size: 

                 740     727      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     225    1497 
   Size:        225    1272
 
BseYI C'CCAG_C

Cuts at:      0     335     613     791     969    1379    1497 
   Size:        335     278     178     178     410     118

  Fragments arranged by size: 

                 410     335     278     178     178     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1289    1497 
   Size:        856     433     208

  Fragments arranged by size: 

                 856     433     208
 
BsiEI CG_ry'CG

Cuts at:      0     844    1497 
   Size:        844     653
 
BsiHKAI G_wGCw'C

Cuts at:      0     108     513     680     776     851    1321    1497 
   Size:        108     405     167      96      75     470     176

  Fragments arranged by size: 

                 470     405     176     167     108      96      75
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1087    1497 
   Size:       1087     410
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     108     513     612     680     745     776     851 
   Size:         66      42     405      99      68      65      31      75

Cuts at:    851    1035    1321    1497 
   Size:        184     286     176

  Fragments arranged by size: 

                 405     286     184     176      99      75      68      66
                  65      42      31
 
BspEI T'CCGG_A

Cuts at:      0     204    1497 
   Size:        204    1293
 
BspHI T'CATG_A

Cuts at:      0    1241    1497 
   Size:       1241     256
 
BsrFI r'CCGG_y

Cuts at:      0     330     529     877    1497 
   Size:        330     199     348     620

  Fragments arranged by size: 

                 620     348     330     199
 
BssSI C'ACGA_G

Cuts at:      0     835    1497 
   Size:        835     662
 
BstBI TT'CG_AA

Cuts at:      0     192    1497 
   Size:        192    1305
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     648    1167    1497 
   Size:         33     615     519     330

  Fragments arranged by size: 

                 615     519     330      33
 
BstYI r'GATC_y

Cuts at:      0     296    1070    1497 
   Size:        296     774     427

  Fragments arranged by size: 

                 774     427     296
 
BtgI C'CryG_G

Cuts at:      0     261     584     994    1312    1497 
   Size:        261     323     410     318     185

  Fragments arranged by size: 

                 410     323     318     261     185
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     378    1497 
   Size:        378    1119
 
BtsI GCAGTG_nn'

Cuts at:      0    1277    1497 
   Size:       1277     220
 
EaeI y'GGCC_r

Cuts at:      0     311    1497 
   Size:        311    1186
 
EarI CTCTTCn'nnn_

Cuts at:      0      96     443    1021    1497 
   Size:         96     347     578     476

  Fragments arranged by size: 

                 578     476     347      96
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     973    1497 
   Size:        973     524
 
EcoICRI GAG'CTC

Cuts at:      0     106     774    1497 
   Size:        106     668     723

  Fragments arranged by size: 

                 723     668     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     472     614     906    1369    1497 
   Size:        340     132     142     292     463     128

  Fragments arranged by size: 

                 463     340     292     142     132     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0     589     930    1497 
   Size:        589     341     567

  Fragments arranged by size: 

                 589     567     341
 
EcoRV GAT'ATC

Cuts at:      0    1227    1497 
   Size:       1227     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1032    1497 
   Size:       1032     465
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175    1497 
   Size:        143      32    1322

  Fragments arranged by size: 

                1322     143      32
 
MspA1I CmG'CkG

Cuts at:      0     586     959     996    1497 
   Size:        586     373      37     501

  Fragments arranged by size: 

                 586     501     373      37
 
NaeI GCC'GGC

Cuts at:      0     332     531     879    1497 
   Size:        332     199     348     618

  Fragments arranged by size: 

                 618     348     332     199
 
NcoI C'CATG_G

Cuts at:      0    1312    1497 
   Size:       1312     185
 
NgoMIV G'CCGG_C

Cuts at:      0     330     529     877    1497 
   Size:        330     199     348     620

  Fragments arranged by size: 

                 620     348     330     199
 
NsiI A_TGCA'T

Cuts at:      0      46    1497 
   Size:         46    1451
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1286    1497 
   Size:       1286     211
 
PmlI CAC'GTG

Cuts at:      0     516    1497 
   Size:        516     981
 
PpuMI rG'GwC_Cy

Cuts at:      0     589    1497 
   Size:        589     908
 
PshAI GACnn'nnGTC

Cuts at:      0       8    1497 
   Size:          8    1489
 
PvuI CG_AT'CG

Cuts at:      0     844    1497 
   Size:        844     653
 
SacI G_AGCT'C

Cuts at:      0     108     776    1497 
   Size:        108     668     721

  Fragments arranged by size: 

                 721     668     108
 
SacII CC_GC'GG

Cuts at:      0     587     997    1497 
   Size:        587     410     500

  Fragments arranged by size: 

                 587     500     410
 
SapI GCTCTTCn'nnn_

Cuts at:      0      96     443    1021    1497 
   Size:         96     347     578     476

  Fragments arranged by size: 

                 578     476     347      96
 
SfcI C'TryA_G

Cuts at:      0     777    1497 
   Size:        777     720
 
SmaI CCC'GGG

Cuts at:      0     673    1497 
   Size:        673     824
 
SmlI C'TyrA_G

Cuts at:      0     107    1497 
   Size:        107    1390
 
StuI AGG'CCT

Cuts at:      0    1340    1497 
   Size:       1340     157
 
StyI C'CwwG_G

Cuts at:      0      66    1312    1497 
   Size:         66    1246     185

  Fragments arranged by size: 

                1246     185      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     496     829    1497 
   Size:        496     333     668

  Fragments arranged by size: 

                 668     496     333
 
Tth111I GACn'n_nGTC

Cuts at:      0    1294    1497 
   Size:       1294     203
 
XmaI C'CCGG_G

Cuts at:      0     671    1497 
   Size:        671     826
 
XmnI GAAnn'nnTTC

Cuts at:      0     919    1497 
   Size:        919     578
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI    ApaLI     ApoI     AvaI    BanII     BbsI
     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BsrFI    BssSI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI
    Hin4I   MspA1I     NaeI     NcoI   NgoMIV     NsiI    PflMI     PmlI
    PpuMI    PshAI     PvuI     SacI    SacII     SapI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI    BglII
     BlpI     BmtI     BplI     BsaI    BsiWI     BsmI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NdeI     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI     XbaI     XcmI     XhoI     ZraI
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