Restriction map for pF1KE0755
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        BalI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BsaAI       BsaBI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
HincII      HindII      HindIII     HinfI       HpaI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvnI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AgeI          274  ACCGGT               A^CCGGT
AhdI          226  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          389  CCTCAGG              CC^T[ACGT]AGG
AspEI         226  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         623  TGCGCA               TGC^GCA
AvrII         886  CCTAGG               C^CTAGG
BamHI         394  GGATCC               G^GATCC
BbeI          878  GGCGCC               GGCGC^C
BbsI          840  GAAGAC               GAAGAC(2/6)
BfaI          887  CTAG                 C^TAG
BlnI          886  CCTAGG               C^CTAGG
BlpI          322  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      322  GCTCAGC              GC^T[ACGT]AGC
BpuAI         840  GAAGAC               GAAGAC(2/6)
BsaI           13  GAGACC               GGTCTC(1/5)
BsaWI         274  ACCGGT               [AT]^CCGG[AT]
BstAPI         62  GCAAGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         394  GGATCC               [GA]^GATC[CT]
Bsu36I        389  CCTCAGG              CC^T[ACGT]AGG
CelII         322  GCTCAGC              GC^T[ACGT]AGC
DpnII         395  GATC                 ^GATC
EaeI          189  TGGCCG               [CT]^GGCC[GA]
Eam1105I      226  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        389  CCTCAGG              CC^T[ACGT]AGG
FseI          533  GGCCGGCC             GGCCGG^CC
FspI          623  TGCGCA               TGC^GCA
KasI          878  GGCGCC               G^GCGCC
MaeI          887  CTAG                 C^TAG
MaeII         704  ACGT                 A^CGT
MaeIII        170  GTGAC                ^GT[ACGT]AC
MboI          395  GATC                 ^GATC
MboII         840  GAAGA                GAAGA(8/7)
MflI          394  GGATCC               [GA]^GATC[CT]
MslI          769  CACAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          878  GGCGCC               GG^CGCC
NcoI          434  CCATGG               C^CATGG
NdeII         395  GATC                 ^GATC
NspI           48  GCATGT               [GA]CATG^[CT]
PinAI         274  ACCGGT               A^CCGGT
Sau3AI        395  GATC                 ^GATC
SfcI          600  CTACAG               C^T[GA][CT]AG
SfoI          878  GGCGCC               GGC^GCC
SgrAI         273  CACCGGTG             C[GA]^CCGG[CT]G
Tsp45I        170  GTGAC                ^GT[GC]AC
TspRI         749  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         394  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          395  GATCC                GGATC(4/5)
              394  GGATC               
AlwNI         182  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              755  CAGGCGCTG           
ApaI          345  GGGCCC               GGGCC^C
              552  GGGCCC              
AvaI          266  CCCGGG               C^[CT]CG[GA]G
              369  CCCGGG              
BbvCI         959  CCTCAGC              CCTCAGC(-5/-2)
              570  GCTGAGG             
BsgI          965  CTGCAC               GTGCAG(16/14)
              493  GTGCAG              
BsoBI         266  CCCGGG               C^[CT]CG[GA]G
              369  CCCGGG              
BsrI          315  ACTGG                ACTGG(1/-1)
              408  CCAGT               
DsaI          434  CCATGG               C^C[GA][CT]GG
              485  CCGTGG              
FauI          515  CCCGC                CCCGC(4/6)
              166  GCGGG               
HaeII         757  GGCGCT               [GA]GCGC^[CT]
              878  GGCGCC              
PflMI         309  CCATCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              754  CCAGGCGCTGG         
SfaNI         403  GATGC                GCATC(5/9)
               32  GCATC               
SmaI          266  CCCGGG               CCC^GGG
              369  CCCGGG              
StuI          335  AGGCCT               AGG^CCT
              383  AGGCCT              
Van91I        309  CCATCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              754  CCAGGCGCTGG         
XcmI          350  CCATCTGCCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              416  CCAAGAGGGGCCTGG     
XmaCI         266  CCCGGG               C^CCGGG
              369  CCCGGG              
XmaI          266  CCCGGG               C^CCGGG
              369  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrFI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MnlI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NgoMIV      NlaIII      NlaIV       PvuII       Sau96I      
ScrFI       StyI        TseI        
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