Restriction map for pF1KE0755
 (Linear) (Six-base) MAPSORT of: KIEE0755.seq  Check: 6106  from: 1  to: 987



KIEE0755.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     859     987 
   Size:        547     312     128

  Fragments arranged by size: 

                 547     312     128
 
AgeI A'CCGG_T

Cuts at:      0     274     987 
   Size:        274     713
 
AhdI GACnn_n'nnGTC

Cuts at:      0     231     987 
   Size:        231     756
 
AleI CACnn'nnGTG

Cuts at:      0     773     987 
   Size:        773     214
 
AlwNI CAG_nnn'CTG

Cuts at:      0     187     760     987 
   Size:        187     573     227

  Fragments arranged by size: 

                 573     227     187
 
ApaI G_GGCC'C

Cuts at:      0     349     556     987 
   Size:        349     207     431

  Fragments arranged by size: 

                 431     349     207
 
AvaI C'yCGr_G

Cuts at:      0     266     369     987 
   Size:        266     103     618

  Fragments arranged by size: 

                 618     266     103
 
AvrII C'CTAG_G

Cuts at:      0     886     987 
   Size:        886     101
 
BamHI G'GATC_C

Cuts at:      0     394     987 
   Size:        394     593
 
BanI G'GyrC_C

Cuts at:      0     277     586     878     987 
   Size:        277     309     292     109

  Fragments arranged by size: 

                 309     292     277     109
 
BanII G_rGCy'C

Cuts at:      0      90     349     556     928     987 
   Size:         90     259     207     372      59

  Fragments arranged by size: 

                 372     259     207      90      59
 
BbeI G_GCGC'C

Cuts at:      0     882     987 
   Size:        882     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     847     987 
   Size:        847     140
 
BbvCI CC'TCA_GC

Cuts at:      0     571     960     987 
   Size:        571     389      27

  Fragments arranged by size: 

                 571     389      27
 
BglI GCCn_nnn'nGGC

Cuts at:      0     113     192     923     987 
   Size:        113      79     731      64

  Fragments arranged by size: 

                 731     113      79      64
 
BlpI GC'TnA_GC

Cuts at:      0     323     987 
   Size:        323     664
 
Bme1580I G_kGCm'C

Cuts at:      0     349     556     589     987 
   Size:        349     207      33     398

  Fragments arranged by size: 

                 398     349     207      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     402     987 
   Size:        402     585
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     405     462     987 
   Size:        405      57     525

  Fragments arranged by size: 

                 525     405      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     148     904     987 
   Size:         31     117     756      83

  Fragments arranged by size: 

                 756     117      83      31
 
Bpu10I CC'TnA_GC

Cuts at:      0     571     960     987 
   Size:        571     389      27

  Fragments arranged by size: 

                 571     389      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       7     987 
   Size:          7     980
 
BsaHI Gr'CG_yC

Cuts at:      0     560     665     879     987 
   Size:        560     105     214     108

  Fragments arranged by size: 

                 560     214     108     105
 
BsaWI w'CCGG_w

Cuts at:      0     274     987 
   Size:        274     713
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     937     967     987 
   Size:        937      30      20

  Fragments arranged by size: 

                 937      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     378     447     664     987 
   Size:        378      69     217     323

  Fragments arranged by size: 

                 378     323     217      69
 
BseYI C'CCAG_C

Cuts at:      0     134     549     760     936     987 
   Size:        134     415     211     176      51

  Fragments arranged by size: 

                 415     211     176     134      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     950     987 
   Size:        514     436      37

  Fragments arranged by size: 

                 514     436      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      90     349     556     589     928     987 
   Size:         90     259     207      33     339      59

  Fragments arranged by size: 

                 339     259     207      90      59      33
 
BsrFI r'CCGG_y

Cuts at:      0     191     243     274     534     987 
   Size:        191      52      31     260     453

  Fragments arranged by size: 

                 453     260     191      52      31
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68     987 
   Size:         68     919
 
BstYI r'GATC_y

Cuts at:      0     394     987 
   Size:        394     593
 
Bsu36I CC'TnA_GG

Cuts at:      0     390     987 
   Size:        390     597
 
BtgI C'CryG_G

Cuts at:      0     434     485     987 
   Size:        434      51     502

  Fragments arranged by size: 

                 502     434      51
 
EaeI y'GGCC_r

Cuts at:      0     189     987 
   Size:        189     798
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      90     205     987 
   Size:         90     115     782

  Fragments arranged by size: 

                 782     115      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     148     547     859     904     987 
   Size:         31     117     399     312      45      83

  Fragments arranged by size: 

                 399     312     117      83      45      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     184     346     423     594     987 
   Size:        184     162      77     171     393

  Fragments arranged by size: 

                 393     184     171     162      77
 
FseI GG_CCGG'CC

Cuts at:      0     538     987 
   Size:        538     449
 
FspI TGC'GCA

Cuts at:      0     625     987 
   Size:        625     362
 
HaeII r_GCGC'y

Cuts at:      0     761     882     987 
   Size:        761     121     105

  Fragments arranged by size: 

                 761     121     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     197     217     229     249     394     426     691     723 
   Size:        197      20      12      20     145      32     265      32

Cuts at:    723     987 
   Size:        264

  Fragments arranged by size: 

                 265     264     197     145      32      32      20      20
                  12
 
KasI G'GCGC_C

Cuts at:      0     878     987 
   Size:        878     109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     662     987 
   Size:        662     325
 
MslI CAynn'nnrTG

Cuts at:      0     773     987 
   Size:        773     214
 
MspA1I CmG'CkG

Cuts at:      0     166     360     518     525     570     605     964     987 
   Size:        166     194     158       7      45      35     359      23

  Fragments arranged by size: 

                 359     194     166     158      45      35      23       7
            
 
NaeI GCC'GGC

Cuts at:      0     193     245     536     987 
   Size:        193      52     291     451

  Fragments arranged by size: 

                 451     291     193      52
 
NarI GG'CG_CC

Cuts at:      0     879     987 
   Size:        879     108
 
NcoI C'CATG_G

Cuts at:      0     434     987 
   Size:        434     553
 
NgoMIV G'CCGG_C

Cuts at:      0     191     243     534     987 
   Size:        191      52     291     453

  Fragments arranged by size: 

                 453     291     191      52
 
NspI r_CATG'y

Cuts at:      0      52     987 
   Size:         52     935
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315     760     987 
   Size:        315     445     227

  Fragments arranged by size: 

                 445     315     227
 
PfoI T'CCnGG_A

Cuts at:      0     143     803     987 
   Size:        143     660     184

  Fragments arranged by size: 

                 660     184     143
 
PspOMI G'GGCC_C

Cuts at:      0     345     552     987 
   Size:        345     207     435

  Fragments arranged by size: 

                 435     345     207
 
PvuII CAG'CTG

Cuts at:      0     360     525     570     605     964     987 
   Size:        360     165      45      35     359      23

  Fragments arranged by size: 

                 360     359     165      45      35      23
 
SfcI C'TryA_G

Cuts at:      0     600     987 
   Size:        600     387
 
SfoI GGC'GCC

Cuts at:      0     880     987 
   Size:        880     107
 
SgrAI Cr'CCGG_yG

Cuts at:      0     274     987 
   Size:        274     713
 
SmaI CCC'GGG

Cuts at:      0     268     371     987 
   Size:        268     103     616

  Fragments arranged by size: 

                 616     268     103
 
StuI AGG'CCT

Cuts at:      0     337     385     987 
   Size:        337      48     602

  Fragments arranged by size: 

                 602     337      48
 
StyI C'CwwG_G

Cuts at:      0     434     581     789     886     975     987 
   Size:        434     147     208      97      89      12

  Fragments arranged by size: 

                 434     208     147      97      89      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     604     987 
   Size:        604     383
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     937     969     987 
   Size:        937      32      18

  Fragments arranged by size: 

                 937      32      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     357     423     987 
   Size:        357      66     564

  Fragments arranged by size: 

                 564     357      66
 
XmaI C'CCGG_G

Cuts at:      0     266     369     987 
   Size:        266     103     618

  Fragments arranged by size: 

                 618     266     103
 

 Enzymes that do cut:

     AcuI     AgeI     AhdI     AleI    AlwNI     ApaI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BsrFI   BstAPI    BstYI
   Bsu36I     BtgI     EaeI     EciI  Eco57MI EcoO109I     FseI     FspI
    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI   PspOMI    PvuII     SfcI
     SfoI    SgrAI     SmaI     StuI     StyI    TaqII     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI    BpuEI
    BsaAI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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