Restriction map for pF1KB7809
 (Linear) (Six-base) MAPSORT of: KIBB7809.seq  Check: 3232  from: 1  to: 1581



KIBB7809.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     255    1581 
   Size:        255    1326
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1263    1431    1581 
   Size:        139    1124     168     150

  Fragments arranged by size: 

                1124     168     150     139
 
AflIII A'CryG_T

Cuts at:      0     479     667    1337    1366    1423    1581 
   Size:        479     188     670      29      57     158

  Fragments arranged by size: 

                 670     479     188     158      57      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363    1581 
   Size:        363    1218
 
ApaI G_GGCC'C

Cuts at:      0      46     141    1581 
   Size:         46      95    1440

  Fragments arranged by size: 

                1440      95      46
 
ApaLI G'TGCA_C

Cuts at:      0     112    1219    1471    1581 
   Size:        112    1107     252     110

  Fragments arranged by size: 

                1107     252     112     110
 
ApoI r'AATT_y

Cuts at:      0    1229    1313    1397    1581 
   Size:       1229      84      84     184

  Fragments arranged by size: 

                1229     184      84      84
 
BamHI G'GATC_C

Cuts at:      0    1061    1581 
   Size:       1061     520
 
BanI G'GyrC_C

Cuts at:      0     243     968    1581 
   Size:        243     725     613

  Fragments arranged by size: 

                 725     613     243
 
BanII G_rGCy'C

Cuts at:      0      46     141    1039    1581 
   Size:         46      95     898     542

  Fragments arranged by size: 

                 898     542      95      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145    1581 
   Size:        145    1436
 
BglII A'GATC_T

Cuts at:      0    1326    1581 
   Size:       1326     255
 
Bme1580I G_kGCm'C

Cuts at:      0      46     116     141    1223    1475    1581 
   Size:         46      70      25    1082     252     106

  Fragments arranged by size: 

                1082     252     106      70      46      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      40     136    1079    1499    1581 
   Size:         40      96     943     420      82

  Fragments arranged by size: 

                 943     420      96      82      40
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     617     755    1581 
   Size:        328     289     138     826

  Fragments arranged by size: 

                 826     328     289     138
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425    1581 
   Size:        425    1156
 
BsaAI yAC'GTr

Cuts at:      0     668    1338    1581 
   Size:        668     670     243

  Fragments arranged by size: 

                 670     668     243
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79    1581 
   Size:         79    1502
 
BseYI C'CCAG_C

Cuts at:      0     644    1581 
   Size:        644     937
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166    1544    1581 
   Size:        166    1378      37

  Fragments arranged by size: 

                1378     166      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     116    1039    1223    1475    1581 
   Size:        116     923     184     252     106

  Fragments arranged by size: 

                 923     252     184     116     106
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     116     141    1039    1223    1475    1581 
   Size:         46      70      25     898     184     252     106

  Fragments arranged by size: 

                 898     252     184     106      70      46      25
 
BspHI T'CATG_A

Cuts at:      0     756    1581 
   Size:        756     825
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     255    1581 
   Size:        255    1326
 
BsrDI GCAATG_nn'

Cuts at:      0     930    1581 
   Size:        930     651
 
BsrGI T'GTAC_A

Cuts at:      0     882    1134    1581 
   Size:        882     252     447

  Fragments arranged by size: 

                 882     447     252
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     250    1581 
   Size:        250    1331
 
BstYI r'GATC_y

Cuts at:      0    1061    1326    1581 
   Size:       1061     265     255

  Fragments arranged by size: 

                1061     265     255
 
BtgI C'CryG_G

Cuts at:      0      23      37    1581 
   Size:         23      14    1544

  Fragments arranged by size: 

                1544      23      14
 
BtsI GCAGTG_nn'

Cuts at:      0    1235    1581 
   Size:       1235     346
 
EaeI y'GGCC_r

Cuts at:      0     290    1581 
   Size:        290    1291
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     563    1581 
   Size:        173     390    1018

  Fragments arranged by size: 

                1018     390     173
 
EcoICRI GAG'CTC

Cuts at:      0    1037    1581 
   Size:       1037     544
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     328     617     755    1263    1431    1581 
   Size:        139     189     289     138     508     168     150

  Fragments arranged by size: 

                 508     289     189     168     150     139     138
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     452    1581 
   Size:        452    1129
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     138     445    1581 
   Size:         42      96     307    1136

  Fragments arranged by size: 

                1136     307      96      42
 
EcoRI G'AATT_C

Cuts at:      0    1229    1313    1397    1581 
   Size:       1229      84      84     184

  Fragments arranged by size: 

                1229     184      84      84
 
HaeII r_GCGC'y

Cuts at:      0     626    1581 
   Size:        626     955
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     858     870     890     902    1110    1142    1581 
   Size:        858      12      20      12     208      32     439

  Fragments arranged by size: 

                 858     439     208      32      20      12      12
 
HindIII A'AGCT_T

Cuts at:      0     461     558    1581 
   Size:        461      97    1023

  Fragments arranged by size: 

                1023     461      97
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1562    1581 
   Size:       1562      19
 
MscI TGG'CCA

Cuts at:      0     292    1581 
   Size:        292    1289
 
MslI CAynn'nnrTG

Cuts at:      0     761    1154    1170    1581 
   Size:        761     393      16     411

  Fragments arranged by size: 

                 761     411     393      16
 
NcoI C'CATG_G

Cuts at:      0      23      37    1581 
   Size:         23      14    1544

  Fragments arranged by size: 

                1544      23      14
 
NdeI CA'TA_TG

Cuts at:      0    1167    1581 
   Size:       1167     414
 
NspI r_CATG'y

Cuts at:      0     483    1370    1427    1581 
   Size:        483     887      57     154

  Fragments arranged by size: 

                 887     483     154      57
 
PciI A'CATG_T

Cuts at:      0     479    1366    1423    1581 
   Size:        479     887      57     158

  Fragments arranged by size: 

                 887     479     158      57
 
PpuMI rG'GwC_Cy

Cuts at:      0     445    1581 
   Size:        445    1136
 
PshAI GACnn'nnGTC

Cuts at:      0    1455    1581 
   Size:       1455     126
 
PspOMI G'GGCC_C

Cuts at:      0      42     137    1581 
   Size:         42      95    1444

  Fragments arranged by size: 

                1444      95      42
 
PstI C_TGCA'G

Cuts at:      0       9    1242    1261    1581 
   Size:          9    1233      19     320

  Fragments arranged by size: 

                1233     320      19       9
 
SacI G_AGCT'C

Cuts at:      0    1039    1581 
   Size:       1039     542
 
SfcI C'TryA_G

Cuts at:      0       5     355    1238    1257    1581 
   Size:          5     350     883      19     324

  Fragments arranged by size: 

                 883     350     324      19       5
 
SmlI C'TyrA_G

Cuts at:      0     440    1581 
   Size:        440    1141
 
StuI AGG'CCT

Cuts at:      0     164     772    1360    1444    1581 
   Size:        164     608     588      84     137

  Fragments arranged by size: 

                 608     588     164     137      84
 
StyI C'CwwG_G

Cuts at:      0      23      37     261    1581 
   Size:         23      14     224    1320

  Fragments arranged by size: 

                1320     224      23      14
 
TatI w'GTAC_w

Cuts at:      0     882    1134    1581 
   Size:        882     252     447

  Fragments arranged by size: 

                 882     447     252
 
XmnI GAAnn'nnTTC

Cuts at:      0     517     772     856    1276    1360    1444    1581 
   Size:        517     255      84     420      84      84     137

  Fragments arranged by size: 

                 517     420     255     137      84      84      84
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI    BamHI
     BanI    BanII     BbsI    BglII Bme1580I     BmrI     BpmI    BpuEI
    BsaAI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstYI     BtgI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I  HindIII
     MmeI     MscI     MslI     NcoI     NdeI     NspI     PciI    PpuMI
    PshAI   PspOMI     PstI     SacI     SfcI     SmlI     StuI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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