Restriction map for pF1KB7774
 (Linear) (Six-base) MAPSORT of: KIBB7774.seq  Check: 527  from: 1  to: 1518



KIBB7774.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579    1251    1518 
   Size:        579     672     267

  Fragments arranged by size: 

                 672     579     267
 
AleI CACnn'nnGTG

Cuts at:      0     309     974    1058    1518 
   Size:        309     665      84     460

  Fragments arranged by size: 

                 665     460     309      84
 
ApaLI G'TGCA_C

Cuts at:      0    1105    1518 
   Size:       1105     413
 
ApoI r'AATT_y

Cuts at:      0     965    1009    1133    1385    1518 
   Size:        965      44     124     252     133

  Fragments arranged by size: 

                 965     252     133     124      44
 
BamHI G'GATC_C

Cuts at:      0    1121    1518 
   Size:       1121     397
 
BfrBI ATG'CAT

Cuts at:      0    1221    1518 
   Size:       1221     297
 
BglII A'GATC_T

Cuts at:      0      61    1518 
   Size:         61    1457
 
Bme1580I G_kGCm'C

Cuts at:      0    1109    1518 
   Size:       1109     409
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     291    1518 
   Size:        291    1227
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     743    1518 
   Size:        190     553     775

  Fragments arranged by size: 

                 775     553     190
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     166     555     723    1395    1518 
   Size:        166     389     168     672     123

  Fragments arranged by size: 

                 672     389     168     166     123
 
BsaWI w'CCGG_w

Cuts at:      0    1295    1518 
   Size:       1295     223
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     257     280     485    1518 
   Size:        257      23     205    1033

  Fragments arranged by size: 

                1033     257     205      23
 
BsiHKAI G_wGCw'C

Cuts at:      0    1109    1518 
   Size:       1109     409
 
BsmI GAATG_Cn'

Cuts at:      0     577    1221    1518 
   Size:        577     644     297

  Fragments arranged by size: 

                 644     577     297
 
Bsp1286I G_dGCh'C

Cuts at:      0    1109    1518 
   Size:       1109     409
 
BspHI T'CATG_A

Cuts at:      0     492    1518 
   Size:        492    1026
 
BsrDI GCAATG_nn'

Cuts at:      0     324     614    1518 
   Size:        324     290     904

  Fragments arranged by size: 

                 904     324     290
 
BsrGI T'GTAC_A

Cuts at:      0    1495    1518 
   Size:       1495      23
 
BstYI r'GATC_y

Cuts at:      0      61     295    1121    1518 
   Size:         61     234     826     397

  Fragments arranged by size: 

                 826     397     234      61
 
Bsu36I CC'TnA_GG

Cuts at:      0     276     343    1518 
   Size:        276      67    1175

  Fragments arranged by size: 

                1175     276      67
 
BtgI C'CryG_G

Cuts at:      0     282    1518 
   Size:        282    1236
 
BtsI GCAGTG_nn'

Cuts at:      0     304    1518 
   Size:        304    1214
 
EaeI y'GGCC_r

Cuts at:      0     209    1518 
   Size:        209    1309
 
EarI CTCTTCn'nnn_

Cuts at:      0     242    1518 
   Size:        242    1276
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     579     743    1251    1518 
   Size:        190     389     164     508     267

  Fragments arranged by size: 

                 508     389     267     190     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0     647    1518 
   Size:        647     871
 
EcoRI G'AATT_C

Cuts at:      0     965    1133    1385    1518 
   Size:        965     168     252     133

  Fragments arranged by size: 

                 965     252     168     133
 
EcoRV GAT'ATC

Cuts at:      0     860    1518 
   Size:        860     658
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     596     628    1518 
   Size:        256      32     308      32     890

  Fragments arranged by size: 

                 890     308     256      32      32
 
HincII GTy'rAC

Cuts at:      0    1291    1518 
   Size:       1291     227
 
HindIII A'AGCT_T

Cuts at:      0     506    1430    1518 
   Size:        506     924      88

  Fragments arranged by size: 

                 924     506      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1518 
   Size:        215    1303
 
MscI TGG'CCA

Cuts at:      0     211    1518 
   Size:        211    1307
 
MslI CAynn'nnrTG

Cuts at:      0     158     309     353     497     974    1058    1518 
   Size:        158     151      44     144     477      84     460

  Fragments arranged by size: 

                 477     460     158     151     144      84      44
 
NcoI C'CATG_G

Cuts at:      0     282    1518 
   Size:        282    1236
 
NsiI A_TGCA'T

Cuts at:      0    1223    1518 
   Size:       1223     295
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      10    1518 
   Size:         10    1508
 
PfoI T'CCnGG_A

Cuts at:      0      45    1518 
   Size:         45    1473
 
SapI GCTCTTCn'nnn_

Cuts at:      0     242    1518 
   Size:        242    1276
 
SfcI C'TryA_G

Cuts at:      0     177     513    1518 
   Size:        177     336    1005

  Fragments arranged by size: 

                1005     336     177
 
StyI C'CwwG_G

Cuts at:      0     282    1518 
   Size:        282    1236
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     480    1518 
   Size:        480    1038
 
TatI w'GTAC_w

Cuts at:      0     742    1495    1518 
   Size:        742     753      23

  Fragments arranged by size: 

                 753     742      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     683     715    1518 
   Size:        683      32     803

  Fragments arranged by size: 

                 803     683      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     424     928    1012    1096    1264    1432    1518 
   Size:        424     504      84      84     168     168      86

  Fragments arranged by size: 

                 504     424     168     168      86      84      84
 

 Enzymes that do cut:

     AcuI     AleI    ApaLI     ApoI    BamHI    BfrBI    BglII Bme1580I
     BplI     BpmI     BsaI    BsaWI    BseRI  BsiHKAI     BsmI Bsp1286I
    BspHI    BsrDI    BsrGI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I   HincII  HindIII
     MmeI     MscI     MslI     NcoI     NsiI    PflMI     PfoI     SapI
     SfcI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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