Restriction map for pF1KB7602
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        Eam1105I    EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        MaeIII      MamI        MfeI        
MflI        MroI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tth111I     Van91I      XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          506  GTAC                 GT^AC
AflIII        136  ACGCGT               A^C[GA][CT]GT
AocI          866  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       620  AGCGCT               AGC^GCT
ApaI           55  GGGCCC               GGGCC^C
AviII         843  TGCGCA               TGC^GCA
BalI          372  TGGCCA               TGG^CCA
BglI          677  GCCAGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI           73  CTGCAC               GTGCAG(16/14)
BsiEI         488  CGGCCG               CG[GA][CT]^CG
BsmFI         465  GGGAC                GGGAC(10/14)
BspMI         227  ACCTGC               ACCTGC(4/8)
BssSI         609  CTCGTG               CACGAG(-5/-1)
BstAPI        454  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         80  CATCC                GGATG(2/0)
Bsu36I        866  CCTCAGG              CC^T[ACGT]AGG
DpnII         222  GATC                 ^GATC
DraIII        606  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          488  CGGCCG               C^GGCCG
EclXI         488  CGGCCG               C^GGCCG
Eco47III      620  AGCGCT               AGC^GCT
Eco52I        488  CGGCCG               C^GGCCG
Eco81I        866  CCTCAGG              CC^T[ACGT]AGG
FokI           80  CATCC                GGATG(9/13)
FspI          843  TGCGCA               TGC^GCA
KspI          103  CCGCGG               CCGC^GG
MaeII         152  ACGT                 A^CGT
MboI          222  GATC                 ^GATC
MluI          136  ACGCGT               A^CGCGT
MluNI         372  TGGCCA               TGG^CCA
MscI          372  TGGCCA               TGG^CCA
NdeII         222  GATC                 ^GATC
NotI          487  GCGGCCGC             GC^GGCCGC
NspI          840  ACATGC               [GA]CATG^[CT]
PaeR7I        157  CTCGAG               C^TCGAG
RsaI          506  GTAC                 GT^AC
SacI          351  GAGCTC               GAGCT^C
SacII         103  CCGCGG               CCGC^GG
Sau3AI        222  GATC                 ^GATC
TaqI          158  TCGA                 T^CGA
Tsp509I       377  AATT                 ^AATT
TthHB8I       158  TCGA                 T^CGA
XbaI          238  TCTAGA               T^CTAGA
XcmI          903  CCAAGGCTACGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          157  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         446  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              455  CAGCGGCTG           
AspHI         154  GTGCTC               G[AT]GC[AT]^C
              351  GAGCTC              
AvaI          157  CTCGAG               C^[CT]CG[GA]G
              354  CTCGGG              
AvaII          66  GGACC                G^G[AT]CC
             1032  GGACC               
BbeI          637  GGCGCC               GGCGC^C
              967  GGCGCC              
BlpI          554  GCTCAGC              GC^T[ACGT]AGC
              789  GCTCAGC             
BpmI          338  CTCCAG               CTGGAG(16/14)
              528  CTGGAG              
Bpu1102I      554  GCTCAGC              GC^T[ACGT]AGC
              789  GCTCAGC             
BsiHKAI       154  GTGCTC               G[AT]GC[AT]^C
              351  GAGCTC              
BsoBI         157  CTCGAG               C^[CT]CG[GA]G
              354  CTCGGG              
BsrBI         294  GAGCGG               CCGCTC(-3/-3)
              300  GAGCGG              
BsrI          340  CCAGT                ACTGG(1/-1)
              861  CCAGT               
CelII         554  GCTCAGC              GC^T[ACGT]AGC
              789  GCTCAGC             
KasI          637  GGCGCC               G^GCGCC
              967  GGCGCC              
MslI          192  CATCTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              835  CACCCACATG          
NarI          637  GGCGCC               GG^CGCC
              967  GGCGCC              
SapI          165  GCTCTTC              GCTCTTC(1/4)
              431  GCTCTTC             
SfoI          637  GGCGCC               GGC^GCC
              967  GGCGCC              
SmlI          157  CTCGAG               C^T[CT][GA]AG
              217  CTCAAG              
TspRI         341  CAGTG                CA[GC]TG[ACGT][ACGT]^
              369  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DsaI        EaeI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NlaIII      NlaIV       PleI        
PstI        PvuII       Sau96I      ScrFI       SfcI        
StyI        TseI        
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