Restriction map for pF1KB7602
 (Linear) (Six-base) MAPSORT of: KIBB7602.seq  Check: 671  from: 1  to: 1062



KIBB7602.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AfeI AGC'GCT

Cuts at:      0     622    1062 
   Size:        622     440
 
AflIII A'CryG_T

Cuts at:      0     136    1062 
   Size:        136     926
 
AlwNI CAG_nnn'CTG

Cuts at:      0     451     460    1062 
   Size:        451       9     602

  Fragments arranged by size: 

                 602     451       9
 
ApaI G_GGCC'C

Cuts at:      0      59    1062 
   Size:         59    1003
 
AvaI C'yCGr_G

Cuts at:      0     157     354    1062 
   Size:        157     197     708

  Fragments arranged by size: 

                 708     197     157
 
BanI G'GyrC_C

Cuts at:      0     576     637     801     967    1062 
   Size:        576      61     164     166      95

  Fragments arranged by size: 

                 576     166     164      95      61
 
BanII G_rGCy'C

Cuts at:      0      59     355     361     535    1062 
   Size:         59     296       6     174     527

  Fragments arranged by size: 

                 527     296     174      59       6
 
BbeI G_GCGC'C

Cuts at:      0     641     971    1062 
   Size:        641     330      91

  Fragments arranged by size: 

                 641     330      91
 
BglI GCCn_nnn'nGGC

Cuts at:      0     683    1062 
   Size:        683     379
 
BlpI GC'TnA_GC

Cuts at:      0     555     790    1062 
   Size:        555     235     272

  Fragments arranged by size: 

                 555     272     235
 
Bme1580I G_kGCm'C

Cuts at:      0      59    1062 
   Size:         59    1003
 
BmgBI CAC'GTC

Cuts at:      0     153    1062 
   Size:        153     909
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     549    1062 
   Size:        323     226     513

  Fragments arranged by size: 

                 513     323     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1062 
   Size:        202     860
 
BsaHI Gr'CG_yC

Cuts at:      0     424     638     968    1062 
   Size:        424     214     330      94

  Fragments arranged by size: 

                 424     330     214      94
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     991    1021    1062 
   Size:        991      30      41

  Fragments arranged by size: 

                 991      41      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220     705     798    1062 
   Size:        220     485      93     264

  Fragments arranged by size: 

                 485     264     220      93
 
BseYI C'CCAG_C

Cuts at:      0      42     318     444    1062 
   Size:         42     276     126     618

  Fragments arranged by size: 

                 618     276     126      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1062 
   Size:         58    1004
 
BsiEI CG_ry'CG

Cuts at:      0     491    1062 
   Size:        491     571
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     355    1062 
   Size:        158     197     707

  Fragments arranged by size: 

                 707     197     158
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     158     355     361     535    1062 
   Size:         59      99     197       6     174     527

  Fragments arranged by size: 

                 527     197     174      99      59       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     236    1062 
   Size:        236     826
 
BsrBI CCG'CTC

Cuts at:      0     296     302    1062 
   Size:        296       6     760

  Fragments arranged by size: 

                 760     296       6
 
BssSI C'ACGA_G

Cuts at:      0     609    1062 
   Size:        609     453
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     460    1062 
   Size:        460     602
 
Bsu36I CC'TnA_GG

Cuts at:      0     867    1062 
   Size:        867     195
 
BtgI C'CryG_G

Cuts at:      0      62      83     103     797     812    1062 
   Size:         62      21      20     694      15     250

  Fragments arranged by size: 

                 694     250      62      21      20      15
 
BtsI GCAGTG_nn'

Cuts at:      0     375    1062 
   Size:        375     687
 
DraIII CAC_nnn'GTG

Cuts at:      0     611    1062 
   Size:        611     451
 
EaeI y'GGCC_r

Cuts at:      0      50      95     372     488     590     675     693    1062 
   Size:         50      45     277     116     102      85      18     369

  Fragments arranged by size: 

                 369     277     116     102      85      50      45      18
            
 
EagI C'GGCC_G

Cuts at:      0     488    1062 
   Size:        488     574
 
EarI CTCTTCn'nnn_

Cuts at:      0     172     438     723    1062 
   Size:        172     266     285     339

  Fragments arranged by size: 

                 339     285     266     172
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     426     975    1062 
   Size:        426     549      87

  Fragments arranged by size: 

                 549     426      87
 
EcoICRI GAG'CTC

Cuts at:      0     353    1062 
   Size:        353     709
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     549    1062 
   Size:        323     226     513

  Fragments arranged by size: 

                 513     323     226
 
FspI TGC'GCA

Cuts at:      0     845    1062 
   Size:        845     217
 
FspAI rTGC'GCAy

Cuts at:      0     845    1062 
   Size:        845     217
 
HaeII r_GCGC'y

Cuts at:      0     166     217     562     624     641     760     971    1062 
   Size:        166      51     345      62      17     119     211      91

  Fragments arranged by size: 

                 345     211     166     119      91      62      51      17
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     233     265     730     762    1024    1056    1062 
   Size:        233      32     465      32     262      32       6

  Fragments arranged by size: 

                 465     262     233      32      32      32       6
 
KasI G'GCGC_C

Cuts at:      0     637     967    1062 
   Size:        637     330      95

  Fragments arranged by size: 

                 637     330      95
 
MluI A'CGCG_T

Cuts at:      0     136    1062 
   Size:        136     926
 
MscI TGG'CCA

Cuts at:      0     374    1062 
   Size:        374     688
 
MslI CAynn'nnrTG

Cuts at:      0     196     839    1062 
   Size:        196     643     223

  Fragments arranged by size: 

                 643     223     196
 
MspA1I CmG'CkG

Cuts at:      0     105     114     147     391     448     457     514     973 
   Size:        105       9      33     244      57       9      57     459

Cuts at:    973    1062 
   Size:         89

  Fragments arranged by size: 

                 459     244     105      89      57      57      33       9
                   9
 
NarI GG'CG_CC

Cuts at:      0     638     968    1062 
   Size:        638     330      94

  Fragments arranged by size: 

                 638     330      94
 
NcoI C'CATG_G

Cuts at:      0      62     797     812    1062 
   Size:         62     735      15     250

  Fragments arranged by size: 

                 735     250      62      15
 
NotI GC'GGCC_GC

Cuts at:      0     488    1062 
   Size:        488     574
 
NspI r_CATG'y

Cuts at:      0     844    1062 
   Size:        844     218
 
PspOMI G'GGCC_C

Cuts at:      0      55    1062 
   Size:         55    1007
 
PspXI vC'TCGA_Gb

Cuts at:      0     157    1062 
   Size:        157     905
 
PstI C_TGCA'G

Cuts at:      0     456     636     745    1062 
   Size:        456     180     109     317

  Fragments arranged by size: 

                 456     317     180     109
 
PvuII CAG'CTG

Cuts at:      0     147     391     514    1062 
   Size:        147     244     123     548

  Fragments arranged by size: 

                 548     244     147     123
 
SacI G_AGCT'C

Cuts at:      0     355    1062 
   Size:        355     707
 
SacII CC_GC'GG

Cuts at:      0     106    1062 
   Size:        106     956
 
SapI GCTCTTCn'nnn_

Cuts at:      0     172     438    1062 
   Size:        172     266     624

  Fragments arranged by size: 

                 624     266     172
 
SfcI C'TryA_G

Cuts at:      0     452     632     741    1062 
   Size:        452     180     109     321

  Fragments arranged by size: 

                 452     321     180     109
 
SfoI GGC'GCC

Cuts at:      0     639     969    1062 
   Size:        639     330      93

  Fragments arranged by size: 

                 639     330      93
 
SmlI C'TyrA_G

Cuts at:      0     157     217    1062 
   Size:        157      60     845

  Fragments arranged by size: 

                 845     157      60
 
StyI C'CwwG_G

Cuts at:      0      62     696     797     812     903    1062 
   Size:         62     634     101      15      91     159

  Fragments arranged by size: 

                 634     159     101      91      62      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      92     851    1062 
   Size:         92     759     211

  Fragments arranged by size: 

                 759     211      92
 
TatI w'GTAC_w

Cuts at:      0     505    1062 
   Size:        505     557
 
XbaI T'CTAG_A

Cuts at:      0     238    1062 
   Size:        238     824
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     910    1062 
   Size:        910     152
 
XhoI C'TCGA_G

Cuts at:      0     157    1062 
   Size:        157     905
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BglI     BlpI Bme1580I    BmgBI     BpmI    BpuEI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BssSI   BstAPI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI     FspI    FspAI    HaeII    Hin4I
     KasI     MluI     MscI     MslI   MspA1I     NarI     NcoI     NotI
     NspI   PspOMI    PspXI     PstI    PvuII     SacI    SacII     SapI
     SfcI     SfoI     SmlI     StyI    TaqII     TatI     XbaI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KpnI
     MfeI     MmeI     NaeI     NdeI   NgoMIV     NheI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PsrI     PvuI    RsrII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XmaI     XmnI     ZraI
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