Restriction map for pF1KE0741
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MluI        MluNI       MroI        MscI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
StuI        SwaI        TspRI       Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AcyI          518  GGCGTC               G[GA]^CG[CT]C
AfaI          768  GTAC                 GT^AC
AlwI          477  GGATC                GGATC(4/5)
AspHI         193  GAGCTC               G[AT]GC[AT]^C
AvaI          104  CCCGGG               C^[CT]CG[GA]G
AvaII         691  GGTCC                G^G[AT]CC
BbsI          127  GTCTTC               GAAGAC(2/6)
BbvCI         615  GCTGAGG              CCTCAGC(-5/-2)
BglII         239  AGATCT               A^GATCT
BpmI          180  CTCCAG               CTGGAG(16/14)
BpuAI         127  GTCTTC               GAAGAC(2/6)
BsaHI         518  GGCGTC               G[GA]^CG[CT]C
BsiHKAI       193  GAGCTC               G[AT]GC[AT]^C
BsmFI         102  GTCCC                GGGAC(10/14)
BsoBI         104  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      767  TGTACA               T^GTACA
BsrGI         767  TGTACA               T^GTACA
BstF5I        186  CATCC                GGATG(2/0)
BstNI         189  CCAGG                CC^[AT]GG
BstYI         239  AGATCT               [GA]^GATC[CT]
DraI          255  TTTAAA               TTT^AAA
DraII         690  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          889  GACGAATTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          935  GAAGAG               CTCTTC(1/4)
EcoO109I      690  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         776  GAATTC               G^AATTC
EcoRII        189  CCAGG                ^CC[AT]GG
EcoT14I        38  CCAAGG               C^C[AT][AT]GG
FokI          186  CATCC                GGATG(9/13)
Hin1I         518  GGCGTC               G[GA]^CG[CT]C
Ksp632I       935  GAAGAG               CTCTTC(1/4)
MaeIII        521  GTCAC                ^GT[ACGT]AC
MfeI          737  CAATTG               C^AATTG
MflI          239  AGATCT               [GA]^GATC[CT]
MspA1I        139  CAGCTG               C[AC]G^C[GT]G
MunI          737  CAATTG               C^AATTG
MvaI          189  CCAGG                CC^[AT]GG
PpuMI         690  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         139  CAGCTG               CAG^CTG
RsaI          768  GTAC                 GT^AC
SacI          193  GAGCTC               GAGCT^C
SmaI          104  CCCGGG               CCC^GGG
SmlI          760  CTTGAG               C^T[CT][GA]AG
SspBI         767  TGTACA               T^GTACA
SspI          505  AATATT               AAT^ATT
StyI           38  CCAAGG               C^C[AT][AT]GG
Tsp45I        521  GTCAC                ^GT[GC]AC
XcmI          158  CCACGGGCCGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         239  AGATCT               [GA]^GATC[CT]
XmaCI         104  CCCGGG               C^CCGGG
XmaI          104  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BanII         107  GGGCTC               G[GA]GC[CT]^C
              193  GAGCTC              
BbvI          138  GCAGC                GCAGC(8/12)
               12  GCTGC               
BfaI          301  CTAG                 C^TAG
              307  CTAG                
BmyI          107  GGGCTC               G[AGT]GC[ACT]^C
              193  GAGCTC              
BsaI           43  GAGACC               GGTCTC(1/5)
              382  GGTCTC              
Bsp1286I      107  GGGCTC               G[AGT]GC[ACT]^C
              193  GAGCTC              
Cfr13I        162  GGGCC                G^G[ACGT]CC
              691  GGTCC               
DsaI          158  CCACGG               C^C[GA][CT]GG
              701  CCGTGG              
HaeIII         30  GGCC                 GG^CC
              163  GGCC                
HapII          50  CCGG                 C^CGG
              105  CCGG                
HgaI          100  GCGTC                GACGC(5/10)
              519  GCGTC               
HpaII          50  CCGG                 C^CGG
              105  CCGG                
HphI          922  GGTGA                GGTGA(8/7)
              386  TCACC               
MaeI          301  CTAG                 C^TAG
              307  CTAG                
MspI           50  CCGG                 C^CGG
              105  CCGG                
NlaIV          89  GGAGCC               GG[ACGT]^[ACGT]CC
              108  GGCTCC              
Sau96I        162  GGGCC                G^G[ACGT]CC
              691  GGTCC               
SfaNI         925  GATGC                GCATC(5/9)
              185  GCATC               
TaqI          341  TCGA                 T^CGA
              780  TCGA                
TseI           12  GCTGC                G^C[AT]GC
              138  GCAGC               
TthHB8I       341  TCGA                 T^CGA
              780  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        ApoI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstUI       Cac8I       CfoI        DdeI        
DpnII       FauI        Fnu4HI      HhaI        HinP1I      
HinfI       ItaI        MboI        MboII       MnlI        
MseI        MslI        MvnI        MwoI        NciI        
NdeII       NlaIII      PleI        Sau3AI      ScrFI       
TfiI        Tru9I       Tsp509I     
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