Restriction map for
pF1KB5796
(Linear) (Six-base) MAPSORT of: KIBB5796.seq Check: 7614 from: 1 to: 2340
KIBB5796.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 2179 2316 2340
Size: 1430 749 137 24
Fragments arranged by size:
1430 749 137 24
AfeI AGC'GCT
Cuts at: 0 1834 2340
Size: 1834 506
AflIII A'CryG_T
Cuts at: 0 1116 2340
Size: 1116 1224
AleI CACnn'nnGTG
Cuts at: 0 158 506 524 806 821 2340
Size: 158 348 18 282 15 1519
Fragments arranged by size:
1519 348 282 158 18 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2200 2232 2340
Size: 2200 32 108
Fragments arranged by size:
2200 108 32
AlwNI CAG_nnn'CTG
Cuts at: 0 19 1272 2340
Size: 19 1253 1068
Fragments arranged by size:
1253 1068 19
ApaI G_GGCC'C
Cuts at: 0 1659 2340
Size: 1659 681
ApoI r'AATT_y
Cuts at: 0 471 2340
Size: 471 1869
BamHI G'GATC_C
Cuts at: 0 1628 2075 2340
Size: 1628 447 265
Fragments arranged by size:
1628 447 265
BanI G'GyrC_C
Cuts at: 0 307 884 1927 2149 2340
Size: 307 577 1043 222 191
Fragments arranged by size:
1043 577 307 222 191
BanII G_rGCy'C
Cuts at: 0 117 270 356 605 1075 1659 2114 2129
Size: 117 153 86 249 470 584 455 15
Cuts at: 2129 2213 2340
Size: 84 127
Fragments arranged by size:
584 470 455 249 153 127 117 86
84 15
BbeI G_GCGC'C
Cuts at: 0 311 1931 2340
Size: 311 1620 409
Fragments arranged by size:
1620 409 311
BbsI GAAGACnn'nnnn_
Cuts at: 0 1035 1181 1632 2102 2340
Size: 1035 146 451 470 238
Fragments arranged by size:
1035 470 451 238 146
BclI T'GATC_A
Cuts at: 0 810 2340
Size: 810 1530
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1663 2340
Size: 281 1382 677
Fragments arranged by size:
1382 677 281
BlpI GC'TnA_GC
Cuts at: 0 1687 2340
Size: 1687 653
Bme1580I G_kGCm'C
Cuts at: 0 152 500 1659 1809 1923 2340
Size: 152 348 1159 150 114 417
Fragments arranged by size:
1159 417 348 152 150 114
BmrI ACTGGGnnnn_n'
Cuts at: 0 815 1188 2340
Size: 815 373 1152
Fragments arranged by size:
1152 815 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1083 1210 2339 2340
Size: 1083 127 1129 1
Fragments arranged by size:
1129 1083 127 1
Bpu10I CC'TnA_GC
Cuts at: 0 595 2340
Size: 595 1745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1474 1618 2065 2340
Size: 287 1187 144 447 275
Fragments arranged by size:
1187 447 287 275 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 443 727 2340
Size: 443 284 1613
Fragments arranged by size:
1613 443 284
BsaAI yAC'GTr
Cuts at: 0 1056 1117 1473 2340
Size: 1056 61 356 867
Fragments arranged by size:
1056 867 356 61
BsaHI Gr'CG_yC
Cuts at: 0 308 1928 2340
Size: 308 1620 412
Fragments arranged by size:
1620 412 308
BsaWI w'CCGG_w
Cuts at: 0 180 1319 1625 2340
Size: 180 1139 306 715
Fragments arranged by size:
1139 715 306 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2200 2230 2340
Size: 2200 30 110
Fragments arranged by size:
2200 110 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 710 2340
Size: 710 1630
BseYI C'CCAG_C
Cuts at: 0 620 2340
Size: 620 1720
BsiEI CG_ry'CG
Cuts at: 0 1221 2340
Size: 1221 1119
BsiHKAI G_wGCw'C
Cuts at: 0 117 356 964 2114 2213 2340
Size: 117 239 608 1150 99 127
Fragments arranged by size:
1150 608 239 127 117 99
Bsp1286I G_dGCh'C
Cuts at: 0 117 152 270 356 500 605 964 1075
Size: 117 35 118 86 144 105 359 111
Cuts at: 1075 1659 1809 1923 2114 2129 2213 2340
Size: 584 150 114 191 15 84 127
Fragments arranged by size:
584 359 191 150 144 127 118 117
114 111 105 86 84 35 15
BspEI T'CCGG_A
Cuts at: 0 180 1319 1625 2340
Size: 180 1139 306 715
Fragments arranged by size:
1139 715 306 180
BspHI T'CATG_A
Cuts at: 0 378 693 2340
Size: 378 315 1647
Fragments arranged by size:
1647 378 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 671 1783 2234 2340
Size: 671 1112 451 106
Fragments arranged by size:
1112 671 451 106
BsrBI CCG'CTC
Cuts at: 0 1112 2270 2340
Size: 1112 1158 70
Fragments arranged by size:
1158 1112 70
BsrFI r'CCGG_y
Cuts at: 0 881 1216 1220 2340
Size: 881 335 4 1120
Fragments arranged by size:
1120 881 335 4
BstXI CCAn_nnnn'nTGG
Cuts at: 0 160 364 397 526 676 709 2136 2340
Size: 160 204 33 129 150 33 1427 204
Fragments arranged by size:
1427 204 204 160 150 129 33 33
BstYI r'GATC_y
Cuts at: 0 1418 1628 2075 2340
Size: 1418 210 447 265
Fragments arranged by size:
1418 447 265 210
Bsu36I CC'TnA_GG
Cuts at: 0 1660 2340
Size: 1660 680
BtgI C'CryG_G
Cuts at: 0 270 444 504 522 543 642 675 834
Size: 270 174 60 18 21 99 33 159
Cuts at: 834 915 942 1066 1559 1793 2340
Size: 81 27 124 493 234 547
Fragments arranged by size:
547 493 270 234 174 159 124 99
81 60 33 27 21 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1482 1731 2340
Size: 1482 249 609
Fragments arranged by size:
1482 609 249
ClaI AT'CG_AT
Cuts at: 0 2288 2340
Size: 2288 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 267 302 2340
Size: 267 35 2038
Fragments arranged by size:
2038 267 35
DraIII CAC_nnn'GTG
Cuts at: 0 147 2340
Size: 147 2193
EaeI y'GGCC_r
Cuts at: 0 1218 1556 1562 2340
Size: 1218 338 6 778
Fragments arranged by size:
1218 778 338 6
EagI C'GGCC_G
Cuts at: 0 1218 2340
Size: 1218 1122
EarI CTCTTCn'nnn_
Cuts at: 0 1267 1627 1786 1792 1873 2318 2340
Size: 1267 360 159 6 81 445 22
Fragments arranged by size:
1267 445 360 159 81 22 6
EcoICRI GAG'CTC
Cuts at: 0 115 354 2112 2211 2340
Size: 115 239 1758 99 129
Fragments arranged by size:
1758 239 129 115 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1083 1210 1430 2179 2316 2339 2340
Size: 1083 127 220 749 137 23 1
Fragments arranged by size:
1083 749 220 137 127 23 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1297 2340
Size: 1297 1043
EcoO109I rG'GnC_Cy
Cuts at: 0 386 530 683 761 1656 1674 1721 2340
Size: 386 144 153 78 895 18 47 619
Fragments arranged by size:
895 619 386 153 144 78 47 18
EcoRV GAT'ATC
Cuts at: 0 779 1155 2340
Size: 779 376 1185
Fragments arranged by size:
1185 779 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1305 2340
Size: 1305 1035
HaeII r_GCGC'y
Cuts at: 0 311 1836 1931 2032 2340
Size: 311 1525 95 101 308
Fragments arranged by size:
1525 311 308 101 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 460 492 1879 1911 2340
Size: 27 433 32 1387 32 429
Fragments arranged by size:
1387 433 429 32 32 27
KasI G'GCGC_C
Cuts at: 0 307 1927 2340
Size: 307 1620 413
Fragments arranged by size:
1620 413 307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 630 2340
Size: 381 249 1710
Fragments arranged by size:
1710 381 249
MscI TGG'CCA
Cuts at: 0 1558 2340
Size: 1558 782
MslI CAynn'nnrTG
Cuts at: 0 158 380 506 524 674 707 806 821
Size: 158 222 126 18 150 33 99 15
Cuts at: 821 1126 1384 1390 1471 1564 1765 1906 2340
Size: 305 258 6 81 93 201 141 434
Fragments arranged by size:
434 305 258 222 201 158 150 141
126 99 93 81 33 18 15 6
MspA1I CmG'CkG
Cuts at: 0 1230 1795 2232 2340
Size: 1230 565 437 108
Fragments arranged by size:
1230 565 437 108
NaeI GCC'GGC
Cuts at: 0 1222 2340
Size: 1222 1118
NarI GG'CG_CC
Cuts at: 0 308 1928 2340
Size: 308 1620 412
Fragments arranged by size:
1620 412 308
NcoI C'CATG_G
Cuts at: 0 270 444 522 543 675 834 942 1559
Size: 270 174 78 21 132 159 108 617
Cuts at: 1559 2340
Size: 781
Fragments arranged by size:
781 617 270 174 159 132 108 78
21
NgoMIV G'CCGG_C
Cuts at: 0 1220 2340
Size: 1220 1120
PasI CC'CwG_GG
Cuts at: 0 1503 1725 1923 2340
Size: 1503 222 198 417
Fragments arranged by size:
1503 417 222 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 1338 2340
Size: 1338 1002
PfoI T'CCnGG_A
Cuts at: 0 991 1307 1706 2340
Size: 991 316 399 634
Fragments arranged by size:
991 634 399 316
PmlI CAC'GTG
Cuts at: 0 1117 2340
Size: 1117 1223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2200 2232 2340
Size: 2200 32 108
Fragments arranged by size:
2200 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 386 530 683 761 1674 1721 2340
Size: 386 144 153 78 913 47 619
Fragments arranged by size:
913 619 386 153 144 78 47
PshAI GACnn'nnGTC
Cuts at: 0 1702 2340
Size: 1702 638
PspOMI G'GGCC_C
Cuts at: 0 1655 2340
Size: 1655 685
PstI C_TGCA'G
Cuts at: 0 2124 2231 2340
Size: 2124 107 109
Fragments arranged by size:
2124 109 107
PvuII CAG'CTG
Cuts at: 0 2232 2340
Size: 2232 108
SacI G_AGCT'C
Cuts at: 0 117 356 2114 2213 2340
Size: 117 239 1758 99 127
Fragments arranged by size:
1758 239 127 117 99
SacII CC_GC'GG
Cuts at: 0 1796 2340
Size: 1796 544
SanDI GG'GwC_CC
Cuts at: 0 386 1721 2340
Size: 386 1335 619
Fragments arranged by size:
1335 619 386
ScaI AGT'ACT
Cuts at: 0 1975 2340
Size: 1975 365
SexAI A'CCwGG_T
Cuts at: 0 1991 2340
Size: 1991 349
SfcI C'TryA_G
Cuts at: 0 558 2120 2227 2340
Size: 558 1562 107 113
Fragments arranged by size:
1562 558 113 107
SfoI GGC'GCC
Cuts at: 0 309 1929 2340
Size: 309 1620 411
Fragments arranged by size:
1620 411 309
SmlI C'TyrA_G
Cuts at: 0 302 1489 1633 2044 2340
Size: 302 1187 144 411 296
Fragments arranged by size:
1187 411 302 296 144
StuI AGG'CCT
Cuts at: 0 2042 2340
Size: 2042 298
StyI C'CwwG_G
Cuts at: 0 270 444 522 543 675 804 834 942
Size: 270 174 78 21 132 129 30 108
Cuts at: 942 1337 1445 1559 2340
Size: 395 108 114 781
Fragments arranged by size:
781 395 270 174 132 129 114 108
108 78 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1079 1082 1138 2340
Size: 1079 3 56 1202
Fragments arranged by size:
1202 1079 56 3
TatI w'GTAC_w
Cuts at: 0 1973 2340
Size: 1973 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 2340
Size: 601 32 1707
Fragments arranged by size:
1707 601 32
Tth111I GACn'n_nGTC
Cuts at: 0 952 1092 2340
Size: 952 140 1248
Fragments arranged by size:
1248 952 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 487 1566 2340
Size: 487 1079 774
Fragments arranged by size:
1079 774 487
XmnI GAAnn'nnTTC
Cuts at: 0 1846 2340
Size: 1846 494
Enzymes that do cut:
AcuI AfeI AflIII AleI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbeI BbsI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI
BtgZI ClaI CspCI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI ScaI SexAI SfcI
SfoI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI
BsaBI BsgI BsiWI BsmI BsmBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtsI DraI DrdI
EciI EcoRI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmaI ZraI