Restriction map for pF1KB6689
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        Asp700I     
Asp718I     AspEI       AspHI       AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         980  GACGTC               GACGT^C
AccI          250  GTAGAC               GT^[AC][GT]AC
AccII         786  CGCG                 CG^CG
AciI          787  GCGG                 CCGC(-3/-1)
AcyI          980  GACGTC               G[GA]^CG[CT]C
AfaI          201  GTAC                 GT^AC
AlwNI         917  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI            4  GGGCCC               GGGCC^C
AseI          823  ATTAAT               AT^TAAT
AsnI          823  ATTAAT               AT^TAAT
AspI          685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          338  CCCGGG               C^[CT]CG[GA]G
BbvI          435  GCTGC                GCAGC(8/12)
BcgI          954  CGAAGTCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          392  CTAG                 C^TAG
BsaHI         980  GACGTC               G[GA]^CG[CT]C
BseRI         395  GAGGAG               GAGGAG(10/8)
BsgI           24  CTGCAC               GTGCAG(16/14)
BsmAI         958  GTCTC                GTCTC(1/5)
BsoBI         338  CCCGGG               C^[CT]CG[GA]G
BspMI          86  ACCTGC               ACCTGC(4/8)
BsrFI         801  ACCGGC               [GA]^CCGG[CT]
BssSI         621  CACGAG               CACGAG(-5/-1)
BstUI         786  CGCG                 CG^CG
Cfr10I        801  ACCGGC               [GA]^CCGG[CT]
DraI           72  TTTAAA               TTT^AAA
EcoT22I       311  ATGCAT               ATGCA^T
Fnu4HI        435  GCTGC                GC^[ACGT]GC
HgaI          346  GACGC                GACGC(5/10)
Hin1I         980  GACGTC               G[GA]^CG[CT]C
ItaI          435  GCTGC                GC^[ACGT]GC
MaeI          392  CTAG                 C^TAG
MaeII         981  ACGT                 A^CGT
MvnI          786  CGCG                 CG^CG
NdeI          242  CATATG               CA^TATG
NsiI          311  ATGCAT               ATGCA^T
NspI          674  GCATGC               [GA]CATG^[CT]
PflFI         685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         823  ATTAAT               AT^TAAT
RsaI          201  GTAC                 GT^AC
SmaI          338  CCCGGG               CCC^GGG
SmlI          101  CTCAAG               C^T[CT][GA]AG
SphI          674  GCATGC               GCATG^C
TaqI          953  TCGA                 T^CGA
TseI          435  GCTGC                G^C[AT]GC
Tth111I       685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       953  TCGA                 T^CGA
XmaCI         338  CCCGGG               C^CCGGG
XmaI          338  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BamHI         352  GGATCC               G^GATCC
              599  GGATCC              
BanII           4  GGGCCC               G[GA]GC[CT]^C
              889  GGGCTC              
BbsI          343  GAAGAC               GAAGAC(2/6)
              983  GTCTTC              
BmyI            4  GGGCCC               G[AGT]GC[ACT]^C
              889  GGGCTC              
BpuAI         343  GAAGAC               GAAGAC(2/6)
              983  GTCTTC              
BsiYI          41  CCTGGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              530  CCTACATTTGG         
BslI           41  CCTGGAAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              530  CCTACATTTGG         
BsmFI         659  GGGAC                GGGAC(10/14)
              569  GTCCC               
Bsp1286I        4  GGGCCC               G[AGT]GC[ACT]^C
              889  GGGCTC              
BstYI         352  GGATCC               [GA]^GATC[CT]
              599  GGATCC              
HaeIII          5  GGCC                 GG^CC
              275  GGCC                
HinfI         180  GAATC                G^A[ACGT]TC
              790  GAATC               
MflI          352  GGATCC               [GA]^GATC[CT]
              599  GGATCC              
NlaIII        675  CATG                 CATG^
              945  CATG                
PpuMI          50  GGGTCCT              [GA]G^G[AT]CC[CT]
              636  AGGACCC             
SfaNI          64  GCATC                GCATC(5/9)
              313  GCATC               
TfiI          180  GAATC                G^A[AT]TC
              790  GAATC               
XhoII         352  GGATCC               [GA]^GATC[CT]
              599  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AluI        AlwI        AvaII       BcnI        BsaJI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      FokI        HapII       HpaII       HphI        
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       Tru9I       
Tsp45I      Tsp509I     TspRI       
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