Restriction map for pF1KB6572
 (Circular) (Six-base) MAPSORT of: pF1KB6572.seq  Check: 2060  from: 1  to: 4313



pF1KB6572  4313 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:    368    1351     368 
   Size:        983    3330
 
Acc65I G'GTAC_C

Cuts at:   1329    1329 
   Size:       4313
 
AclI AA'CG_TT

Cuts at:   4153    4153 
   Size:       4313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    292     668     883    2138    2570    3317    3585     292 
   Size:        376     215    1255     432     747     268    1020

  Fragments arranged by size: 

                1255    1020     747     432     376     268     215
 
AflIII A'CryG_T

Cuts at:   1184    1665    2769    3746    1184 
   Size:        481    1104     977    1751

  Fragments arranged by size: 

                1751    1104     977     481
 
AgeI A'CCGG_T

Cuts at:   2701    2701 
   Size:       4313
 
AleI CACnn'nnGTG

Cuts at:    349     499     349 
   Size:        150    4163
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    283     283 
   Size:       4313
 
AlwNI CAG_nnn'CTG

Cuts at:    881    1618    3185     881 
   Size:        737    1567    2009

  Fragments arranged by size: 

                2009    1567     737
 
ApaI G_GGCC'C

Cuts at:    285    1049     285 
   Size:        764    3549
 
ApaLI G'TGCA_C

Cuts at:   3083    3083 
   Size:       4313
 
ApoI r'AATT_y

Cuts at:    396     957    1317    3666    3922     396 
   Size:        561     360    2349     256     787

  Fragments arranged by size: 

                2349     787     561     360     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4313
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4313
 
AvaI C'yCGr_G

Cuts at:   1058    1333    1058 
   Size:        275    4038
 
BamHI G'GATC_C

Cuts at:    773    1338     773 
   Size:        565    3748
 
BanI G'GyrC_C

Cuts at:    172    1329    1743    1991    2026     172 
   Size:       1157     414     248      35    2459

  Fragments arranged by size: 

                2459    1157     414     248      35
 
BanII G_rGCy'C

Cuts at:    285     544    1049    1327    2357     285 
   Size:        259     505     278    1030    2241

  Fragments arranged by size: 

                2241    1030     505     278     259
 
BbeI G_GCGC'C

Cuts at:   1747    1995    1747 
   Size:        248    4065
 
BbsI GAAGACnn'nnnn_

Cuts at:    231     955     231 
   Size:        724    3589
 
BciVI GTATCCnnnnn_n'

Cuts at:   2211    2972    4313    2211 
   Size:        761    1341    2211

  Fragments arranged by size: 

                2211    1341     761
 
BclI T'GATC_A

Cuts at:    566    1833     566 
   Size:       1267    3046
 
BglI GCCn_nnn'nGGC

Cuts at:    181     181 
   Size:       4313
 
BglII A'GATC_T

Cuts at:   1828    3865    1828 
   Size:       2037    2276
 
BlpI GC'TnA_GC

Cuts at:    988    1425     988 
   Size:        437    3876
 
Bme1580I G_kGCm'C

Cuts at:    285    1049    1938    2031    3087     285 
   Size:        764     889      93    1056    1511

  Fragments arranged by size: 

                1511    1056     889     764      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1525    1606    1941    4085    1525 
   Size:         81     335    2144    1753

  Fragments arranged by size: 

                2144    1753     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2680    2680 
   Size:       4313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    394     877     394 
   Size:        483    3830
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1276    1489    2368    2860    3158    1276 
   Size:        213     879     492     298    2431

  Fragments arranged by size: 

                2431     879     492     298     213
 
BsaI GGTCTCn'nnnn_

Cuts at:   4017    4017 
   Size:       4313
 
BsaAI yAC'GTr

Cuts at:   1185    1227    1666    1185 
   Size:         42     439    3832

  Fragments arranged by size: 

                3832     439      42
 
BsaBI GATnn'nnATC

Cuts at:      6    1827    3864       6 
   Size:       1821    2037     455

  Fragments arranged by size: 

                2037    1821     455
 
BsaHI Gr'CG_yC

Cuts at:   1744    1992    2694    4190    1744 
   Size:        248     702    1496    1867

  Fragments arranged by size: 

                1867    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1277    1502    2023    2701    2975    3122    3533    1277 
   Size:        225     521     678     274     147     411    2057

  Fragments arranged by size: 

                2057     678     521     411     274     225     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    704     734     822     852     704 
   Size:         30      88      30    4165

  Fragments arranged by size: 

                4165      88      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    938     941     958     938 
   Size:          3      17    4293

  Fragments arranged by size: 

                4293      17       3
 
BseYI C'CCAG_C

Cuts at:   1041    1218    1738    2496    3073    3569    1041 
   Size:        177     520     758     577     496    1785

  Fragments arranged by size: 

                1785     758     577     520     496     177
 
BsiEI CG_ry'CG

Cuts at:    104     575    1855    1901    3109     104 
   Size:        471    1280      46    1208    1308

  Fragments arranged by size: 

                1308    1280    1208     471      46
 
BsiHKAI G_wGCw'C

Cuts at:    544    1327    2105    2295    3087     544 
   Size:        783     778     190     792    1770

  Fragments arranged by size: 

                1770     792     783     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    756     756 
   Size:       4313
 
Bsp1286I G_dGCh'C

Cuts at:    285     544    1049    1327    1938    2031    2105    2295    2357 
   Size:        259     505     278     611      93      74     190      62

Cuts at:   2357    3087     285 
   Size:        730    1511

  Fragments arranged by size: 

                1511     730     611     505     278     259     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   1277    3533    1277 
   Size:       2256    2057
 
BspHI T'CATG_A

Cuts at:    440     563    3489     440 
   Size:        123    2926    1264

  Fragments arranged by size: 

                2926    1264     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    710    1363    1577    1879    2260     710 
   Size:        653     214     302     381    2763

  Fragments arranged by size: 

                2763     653     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     279    2605    2659    4143    4308      50 
   Size:        229    2326      54    1484     165      55

  Fragments arranged by size: 

                2326    1484     229     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    252    1020    2225     252 
   Size:        768    1205    2340

  Fragments arranged by size: 

                2340    1205     768
 
BsrFI r'CCGG_y

Cuts at:   2311    2492    2701    2311 
   Size:        181     209    3923

  Fragments arranged by size: 

                3923     209     181
 
BsrGI T'GTAC_A

Cuts at:    448     448 
   Size:       4313
 
BssHII G'CGCG_C

Cuts at:    218     218 
   Size:       4313
 
BssSI C'ACGA_G

Cuts at:   2584    2942    2584 
   Size:        358    3955
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1690    1690 
   Size:       4313
 
BstBI TT'CG_AA

Cuts at:   1002    2674    1002 
   Size:       1672    2641
 
BstXI CCAn_nnnn'nTGG

Cuts at:    130     130 
   Size:       4313
 
BstYI r'GATC_y

Cuts at:      1     773    1295    1338    1805    1828    2163    2409    3421 
   Size:        772     522      43     467      23     335     246    1012

Cuts at:   3421    3507    3519    3865       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     772     522     467     449     346     335     246
                  86      43      23      12
 
BstZ17I GTA'TAC

Cuts at:    369     369 
   Size:       4313
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4313
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2263    2444    2607    2263 
   Size:        181     163    3969

  Fragments arranged by size: 

                3969     181     163
 
BtsI GCAGTG_nn'

Cuts at:    494     506     890    1167    1556     494 
   Size:         12     384     277     389    3251

  Fragments arranged by size: 

                3251     389     384     277      12
 
DraI TTT'AAA

Cuts at:   1312    1312 
   Size:       4313
 
DrdI GACnn_nn'nnGTC

Cuts at:    643     791    2019    2877     643 
   Size:        148    1228     858    2079

  Fragments arranged by size: 

                2079    1228     858     148
 
EaeI y'GGCC_r

Cuts at:    132     230     341     572    1100    1898    2072    2463    2490 
   Size:         98     111     231     528     798     174     391      27

Cuts at:   2490    3835     132 
   Size:       1345     610

  Fragments arranged by size: 

                1345     798     610     528     391     231     174     111
                  98      27
 
EagI C'GGCC_G

Cuts at:    572    1898     572 
   Size:       1326    2987
 
EarI CTCTTCn'nnn_

Cuts at:    614     687    2336    2546     614 
   Size:         73    1649     210    2381

  Fragments arranged by size: 

                2381    1649     210      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2521    2831    2977    4123    2521 
   Size:        310     146    1146    2711

  Fragments arranged by size: 

                2711    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    542    1325     542 
   Size:        783    3530
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    292     394     668     877     883    2138    2570    3317    3585 
   Size:        102     274     209       6    1255     432     747     268

Cuts at:   3585     292 
   Size:       1020

  Fragments arranged by size: 

                1255    1020     747     432     274     268     209     102
                   6
 
EcoO109I rG'GnC_Cy

Cuts at:    282     636    1045    1046    1115    1452     282 
   Size:        354     409       1      69     337    3143

  Fragments arranged by size: 

                3143     409     354     337      69       1
 
EcoRI G'AATT_C

Cuts at:    957    1317     957 
   Size:        360    3953
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1008    2332    1008 
   Size:       1324    2989
 
FspI TGC'GCA

Cuts at:   2094    2094 
   Size:       4313
 
HaeII r_GCGC'y

Cuts at:    982    1238    1747    1995    3017    3996     982 
   Size:        256     509     248    1022     979    1299

  Fragments arranged by size: 

                1299    1022     979     509     256     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    531     563     704     736     765     797    2155    2185    2187 
   Size:         32     141      32      29      32    1358      30       2

Cuts at:   2187    2217     531 
   Size:         30    2627

  Fragments arranged by size: 

                2627    1358     141      32      32      32      30      30
                  29       2
 
HincII GTy'rAC

Cuts at:   1352    1352 
   Size:       4313
 
KasI G'GCGC_C

Cuts at:   1743    1991    1743 
   Size:        248    4065
 
KpnI G_GTAC'C

Cuts at:   1333    1333 
   Size:       4313
 
MluI A'CGCG_T

Cuts at:   3746    3746 
   Size:       4313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    683    2984    3168     683 
   Size:       2301     184    1828

  Fragments arranged by size: 

                2301    1828     184
 
MscI TGG'CCA

Cuts at:    134     232     343    1102    2074     134 
   Size:         98     111     759     972    2373

  Fragments arranged by size: 

                2373     972     759     111      98
 
MslI CAynn'nnrTG

Cuts at:    265     349     499    2302    2429     265 
   Size:         84     150    1803     127    2149

  Fragments arranged by size: 

                2149    1803     150     127      84
 
MspA1I CmG'CkG

Cuts at:    126    1424    1738    2098    3111    3356     126 
   Size:       1298     314     360    1013     245    1083

  Fragments arranged by size: 

                1298    1083    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2494    2494 
   Size:       4313
 
NarI GG'CG_CC

Cuts at:   1744    1992    1744 
   Size:        248    4065
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4313
 
NgoMIV G'CCGG_C

Cuts at:   2492    2492 
   Size:       4313
 
NspI r_CATG'y

Cuts at:    196    1181    1366    1866    2773     196 
   Size:        985     185     500     907    1736

  Fragments arranged by size: 

                1736     985     907     500     185
 
PasI CC'CwG_GG

Cuts at:    359     359 
   Size:       4313
 
PciI A'CATG_T

Cuts at:   2769    2769 
   Size:       4313
 
PflMI CCAn_nnn'nTGG

Cuts at:   1098    1214    1098 
   Size:        116    4197
 
PmeI GTTT'AAAC

Cuts at:   1312    1312 
   Size:       4313
 
PmlI CAC'GTG

Cuts at:   1185    1185 
   Size:       4313
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3452    3484    3452 
   Size:         32    4281
 
PpuMI rG'GwC_Cy

Cuts at:    636     636 
   Size:       4313
 
PspOMI G'GGCC_C

Cuts at:    281    1045     281 
   Size:        764    3549
 
PstI C_TGCA'G

Cuts at:    290     501    1360    2045     290 
   Size:        211     859     685    2558

  Fragments arranged by size: 

                2558     859     685     211
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4313
 
PvuII CAG'CTG

Cuts at:    126    1738    2098     126 
   Size:       1612     360    2341

  Fragments arranged by size: 

                2341    1612     360
 
RsrII CG'GwC_CG

Cuts at:   2508    2508 
   Size:       4313
 
SacI G_AGCT'C

Cuts at:    544    1327     544 
   Size:        783    3530
 
SalI G'TCGA_C

Cuts at:   1350    1350 
   Size:       4313
 
SanDI GG'GwC_CC

Cuts at:    636     636 
   Size:       4313
 
SapI GCTCTTCn'nnn_

Cuts at:   2336    2546    2336 
   Size:        210    4103
 
SbfI CC_TGCA'GG

Cuts at:   1360    1360 
   Size:       4313
 
ScaI AGT'ACT

Cuts at:   3830    3830 
   Size:       4313
 
SfcI C'TryA_G

Cuts at:     33     286     497     873    1356    2041    3034    3225      33 
   Size:        253     211     376     483     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     483     376     253     211     191
            
 
SfoI GGC'GCC

Cuts at:   1745    1993    1745 
   Size:        248    4065
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4313
 
SmaI CCC'GGG

Cuts at:   1335    1335 
   Size:       4313
 
SmlI C'TyrA_G

Cuts at:   1291    1468    2383    2875    3137    1291 
   Size:        177     915     492     262    2467

  Fragments arranged by size: 

                2467     915     492     262     177
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4313
 
SphI G_CATG'C

Cuts at:   1366    1866    1366 
   Size:        500    3813
 
StuI AGG'CCT

Cuts at:    813     813 
   Size:       4313
 
StyI C'CwwG_G

Cuts at:    107     347     896    1447    1801     107 
   Size:        240     549     551     354    2619

  Fragments arranged by size: 

                2619     551     549     354     240
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    351     889    1896    2029    2488    2697     351 
   Size:        538    1007     133     459     209    1967

  Fragments arranged by size: 

                1967    1007     538     459     209     133
 
TatI w'GTAC_w

Cuts at:    448    3828     448 
   Size:       3380     933
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3830    3862    3830 
   Size:         32    4281
 
Tth111I GACn'n_nGTC

Cuts at:   2110    2110 
   Size:       4313
 
XbaI T'CTAG_A

Cuts at:   1344    1344 
   Size:       4313
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    741    1215    1808     741 
   Size:        474     593    3246

  Fragments arranged by size: 

                3246     593     474
 
XmaI C'CCGG_G

Cuts at:   1333    1333 
   Size:       4313
 
XmnI GAAnn'nnTTC

Cuts at:   1285    1285 
   Size:       4313
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I     BsgI
    BsiWI     BsmI   BstEII   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PfoI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
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