Restriction map for pF1KB5979
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BbeI        BbvCI       
BcgI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII          20  CGCG                 CG^CG
Alw44I        670  GTGCAC               G^TGCAC
Aor51HI       148  AGCGCT               AGC^GCT
ApaI          989  GGGCCC               GGGCC^C
ApaLI         670  GTGCAC               G^TGCAC
Asp700I       306  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          419  TGGCCA               TGG^CCA
BamHI         396  GGATCC               G^GATCC
BanI          432  GGTGCC               G^G[CT][GA]CC
BbrPI         673  CACGTG               CAC^GTG
BbsI         1279  GAAGAC               GAAGAC(2/6)
BciVI        1536  GTATCC               GTATCC(6/5)
BcnI          537  CCCGG                CC^[GC]GG
BpuAI        1279  GAAGAC               GAAGAC(2/6)
BsaAI         673  CACGTG               [CT]AC^GT[GA]
BsaBI        1291  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1067  GAATGC               GAATGC(1/-1)
BspDI         424  ATCGAT               AT^CGAT
BspHI        1217  TCATGA               T^CATGA
BstAPI        642  GCAATCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1251  GGTCACC              G^GT[ACGT]ACC
BstPI        1251  GGTCACC              G^GT[ACGT]ACC
BstUI          20  CGCG                 CG^CG
BstXI         422  CCATCGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          424  ATCGAT               AT^CGAT
DraIII        944  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          419  TGGCCA               [CT]^GGCC[GA]
Eco47III      148  AGCGCT               AGC^GCT
EcoO65I      1251  GGTCACC              G^GT[ACGT]ACC
EcoT22I       649  ATGCAT               ATGCA^T
HaeII         148  AGCGCT               [GA]GCGC^[CT]
MamI         1291  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         419  TGGCCA               TGG^CCA
MscI          419  TGGCCA               TGG^CCA
MslI          423  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        231  CAGCGG               C[AC]G^C[GT]G
MvnI           20  CGCG                 CG^CG
NciI          537  CCCGG                CC^[GC]GG
NcoI          895  CCATGG               C^CATGG
NsiI          649  ATGCAT               ATGCA^T
PmaCI         673  CACGTG               CAC^GTG
PmlI          673  CACGTG               CAC^GTG
PshAI        1486  GACGACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          152  CTGCAG               CTGCA^G
RcaI         1217  TCATGA               T^CATGA
SacI            9  GAGCTC               GAGCT^C
SapI          159  GAAGAGC              GCTCTTC(1/4)
SspI         1354  AATATT               AAT^ATT
StuI          901  AGGCCT               AGG^CCT
XbaI         1447  TCTAGA               T^CTAGA
XmnI          306  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AspHI           9  GAGCTC               G[AT]GC[AT]^C
              670  GTGCAC              
BclI          335  TGATCA               T^GATCA
             1290  TGATCA              
BpmI          968  CTCCAG               CTGGAG(16/14)
             1284  CTCCAG              
BseRI         130  GAGGAG               GAGGAG(10/8)
              133  GAGGAG              
BsiHKAI         9  GAGCTC               G[AT]GC[AT]^C
              670  GTGCAC              
BsmFI         325  GGGAC                GGGAC(10/14)
              705  GTCCC               
BspMI        1088  ACCTGC               ACCTGC(4/8)
             1103  ACCTGC              
BsrFI         435  GCCGGT               [GA]^CCGG[CT]
             1164  ACCGGC              
BstYI         292  GGATCT               [GA]^GATC[CT]
              396  GGATCC              
Cfr10I        435  GCCGGT               [GA]^CCGG[CT]
             1164  ACCGGC              
Cfr13I        989  GGGCC                G^G[ACGT]CC
              990  GGCCC               
DraI          751  TTTAAA               TTT^AAA
              922  TTTAAA              
DraII         988  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              989  GGGCCCC             
DsaI           32  CCGTGG               C^C[GA][CT]GG
              895  CCATGG              
EcoO109I      988  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              989  GGGCCCC             
FauI          105  GCGGG                CCCGC(4/6)
              349  GCGGG               
FbaI          335  TGATCA               T^GATCA
             1290  TGATCA              
HincII        697  GTTGAC               GT[CT]^[GA]AC
             1099  GTCAAC              
HindII        697  GTTGAC               GT[CT]^[GA]AC
             1099  GTCAAC              
MaeII         461  ACGT                 A^CGT
              674  ACGT                
MflI          292  GGATCT               [GA]^GATC[CT]
              396  GGATCC              
PleI          541  GACTC                GAGTC(4/5)
             1282  GACTC               
Sau96I        989  GGGCC                G^G[ACGT]CC
              990  GGCCC               
SfaNI         567  GATGC                GCATC(5/9)
              512  GCATC               
SfcI          152  CTGCAG               C^T[GA][CT]AG
              453  CTATAG              
TaqI          425  TCGA                 T^CGA
              917  TCGA                
TthHB8I       425  TCGA                 T^CGA
              917  TCGA                
XcmI          605  CCAACAATGAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1129  CCAACTAATTTTTGG     
XhoII         292  GGATCT               [GA]^GATC[CT]
              396  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
BanII       BbvI        BfaI        BmyI        BsaI        
BsaJI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      ScrFI       StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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