Restriction map for pF1KB5856
 (Linear) (Six-base) MAPSORT of: KIBB5856.seq  Check: 6186  from: 1  to: 1467



KIBB5856.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428    1094    1467 
   Size:        428     666     373

  Fragments arranged by size: 

                 666     428     373
 
AflIII A'CryG_T

Cuts at:      0     663    1380    1467 
   Size:        663     717      87

  Fragments arranged by size: 

                 717     663      87
 
ApoI r'AATT_y

Cuts at:      0     207     285     352     627    1285    1459    1467 
   Size:        207      78      67     275     658     174       8

  Fragments arranged by size: 

                 658     275     207     174      78      67       8
 
AseI AT'TA_AT

Cuts at:      0     818    1467 
   Size:        818     649
 
AvaI C'yCGr_G

Cuts at:      0    1098    1467 
   Size:       1098     369
 
BanI G'GyrC_C

Cuts at:      0      58    1467 
   Size:         58    1409
 
BanII G_rGCy'C

Cuts at:      0    1334    1467 
   Size:       1334     133
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1016    1467 
   Size:       1016     451
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     627    1467 
   Size:        627     840
 
BclI T'GATC_A

Cuts at:      0     264    1215    1467 
   Size:        264     951     252

  Fragments arranged by size: 

                 951     264     252
 
BfrBI ATG'CAT

Cuts at:      0    1002    1467 
   Size:       1002     465
 
BlpI GC'TnA_GC

Cuts at:      0     781    1467 
   Size:        781     686
 
Bme1580I G_kGCm'C

Cuts at:      0      61    1467 
   Size:         61    1406
 
BmgBI CAC'GTC

Cuts at:      0     926    1092    1467 
   Size:        926     166     375

  Fragments arranged by size: 

                 926     375     166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     965    1179    1206    1467 
   Size:        965     214      27     261

  Fragments arranged by size: 

                 965     261     214      27
 
BsaBI GATnn'nnATC

Cuts at:      0     482    1467 
   Size:        482     985
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     424    1467 
   Size:        424    1043
 
BsiHKAI G_wGCw'C

Cuts at:      0    1334    1467 
   Size:       1334     133
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     455    1467 
   Size:        455    1012
 
Bsp1286I G_dGCh'C

Cuts at:      0      61    1334    1467 
   Size:         61    1273     133

  Fragments arranged by size: 

                1273     133      61
 
BspHI T'CATG_A

Cuts at:      0     422    1467 
   Size:        422    1045
 
BsrGI T'GTAC_A

Cuts at:      0     512    1467 
   Size:        512     955
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1254    1467 
   Size:       1254     213
 
BstYI r'GATC_y

Cuts at:      0      66     545    1392    1467 
   Size:         66     479     847      75

  Fragments arranged by size: 

                 847     479      75      66
 
BtgI C'CryG_G

Cuts at:      0      62    1467 
   Size:         62    1405
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1158    1467 
   Size:       1158     309
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1344    1379    1467 
   Size:       1344      35      88

  Fragments arranged by size: 

                1344      88      35
 
DraI TTT'AAA

Cuts at:      0    1181    1467 
   Size:       1181     286
 
EarI CTCTTCn'nnn_

Cuts at:      0     962    1467 
   Size:        962     505
 
EcoICRI GAG'CTC

Cuts at:      0    1332    1467 
   Size:       1332     135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     965    1094    1179    1206    1467 
   Size:        428     537     129      85      27     261

  Fragments arranged by size: 

                 537     428     261     129      85      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165    1467 
   Size:        165    1302
 
EcoRI G'AATT_C

Cuts at:      0     207     627    1467 
   Size:        207     420     840

  Fragments arranged by size: 

                 840     420     207
 
EcoRV GAT'ATC

Cuts at:      0     399    1467 
   Size:        399    1068
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     754     899    1467 
   Size:        754     145     568

  Fragments arranged by size: 

                 754     568     145
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     961     993    1316    1348    1467 
   Size:        961      32     323      32     119

  Fragments arranged by size: 

                 961     323     119      32      32
 
MfeI C'AATT_G

Cuts at:      0     107    1467 
   Size:        107    1360
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     297    1467 
   Size:        297    1170
 
MslI CAynn'nnrTG

Cuts at:      0     272     508    1467 
   Size:        272     236     959

  Fragments arranged by size: 

                 959     272     236
 
MspA1I CmG'CkG

Cuts at:      0      64    1250    1467 
   Size:         64    1186     217

  Fragments arranged by size: 

                1186     217      64
 
NsiI A_TGCA'T

Cuts at:      0    1004    1467 
   Size:       1004     463
 
NspI r_CATG'y

Cuts at:      0     667    1002    1384    1467 
   Size:        667     335     382      83

  Fragments arranged by size: 

                 667     382     335      83
 
PciI A'CATG_T

Cuts at:      0     663    1380    1467 
   Size:        663     717      87

  Fragments arranged by size: 

                 717     663      87
 
PfoI T'CCnGG_A

Cuts at:      0    1017    1467 
   Size:       1017     450
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1116    1148    1467 
   Size:       1116      32     319

  Fragments arranged by size: 

                1116     319      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     165    1467 
   Size:        165    1302
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     717     749    1467 
   Size:        717      32     718

  Fragments arranged by size: 

                 718     717      32
 
PvuII CAG'CTG

Cuts at:      0    1250    1467 
   Size:       1250     217
 
SacI G_AGCT'C

Cuts at:      0    1334    1467 
   Size:       1334     133
 
SacII CC_GC'GG

Cuts at:      0      65    1467 
   Size:         65    1402
 
SapI GCTCTTCn'nnn_

Cuts at:      0     962    1467 
   Size:        962     505
 
ScaI AGT'ACT

Cuts at:      0     637    1467 
   Size:        637     830
 
SmaI CCC'GGG

Cuts at:      0    1100    1467 
   Size:       1100     367
 
SphI G_CATG'C

Cuts at:      0    1002    1467 
   Size:       1002     465
 
SspI AAT'ATT

Cuts at:      0     237     258     845    1083    1467 
   Size:        237      21     587     238     384

  Fragments arranged by size: 

                 587     384     238     237      21
 
TatI w'GTAC_w

Cuts at:      0     512     635    1467 
   Size:        512     123     832

  Fragments arranged by size: 

                 832     512     123
 
Tth111I GACn'n_nGTC

Cuts at:      0    1093    1467 
   Size:       1093     374
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1162    1467 
   Size:       1162     305
 
XmaI C'CCGG_G

Cuts at:      0    1098    1467 
   Size:       1098     369
 
XmnI GAAnn'nnTTC

Cuts at:      0    1082    1203    1467 
   Size:       1082     121     264

  Fragments arranged by size: 

                1082     264     121
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI     AvaI     BanI    BanII     BbsI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BpmI    BsaBI
    BseRI  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrGI    BstXI    BstYI
     BtgI    BtgZI    CspCI     DraI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I     MfeI     MmeI     MslI   MspA1I
     NsiI     NspI     PciI     PfoI     PpiI    PpuMI     PsrI    PvuII
     SacI    SacII     SapI     ScaI     SmaI     SphI     SspI     TatI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BglI
    BglII     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII     SalI    SanDI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI
     StuI     StyI     SwaI    TaqII     TstI     XbaI     XhoI     ZraI
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